{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T14:37:35Z","timestamp":1787236655644,"version":"build-2736575974"},"reference-count":51,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,7,9]],"date-time":"2025-07-09T00:00:00Z","timestamp":1752019200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2025,7,9]],"date-time":"2025-07-09T00:00:00Z","timestamp":1752019200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012331","name":"LEO Fondet","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012331","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>\n                    <jats:styled-content style=\"fixed-case\">ABSTRACT<\/jats:styled-content>\n                  <\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Immune mechanisms underlying elicitation in allergic contact dermatitis (ACD) have yet to be fully elucidated. Previous studies have shown a double\u2010faceted nature of ACD with both common biomarkers among different allergens and allergen\u2010specific imprinting, albeit with discordance in terms of relevant pathways involved. Several factors, including co\u2010existing atopic dermatitis, may influence immune reactions. We aim to characterize molecular signatures and their immune mechanisms of different relevant allergens (nickel, 2\u2010hydroxyethylmethacrylate [2\u2010HEMA], methylisothiazolinone [MIT], formaldehyde) in strong and extreme positive (2\/3+) patch test reactions of patients without atopic dermatitis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A transcriptomic analysis of 40 skin biopsies of ACD reactions (11 nickel, 10 MIT, 10 2\u2010HEMA, 9 formaldehyde) and 19 controls (petrolatum\u2010occluded skin) was performed using RNA sequencing. Differentially expressed genes (DEG) were assessed, and enriched functional pathways were obtained with an over\u2010representation analysis for allergens.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      ACD molecular profiling revealed a strong, common imprinting of DEG among allergens versus controls (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u2009814), with further partially shared DEG among allergens (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u2009664) and allergen\u2010specific DEG (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u2009430). The most relevant shared pathways were associated with immune adaptive and innate responses. All allergens exhibited mixed effector immune responses, mainly type 1 and 3 immunity, and, to a lesser extent, type 2 immunity. Furthermore, partially shared and unique DEG were associated with further inflammatory pathways, particularly for nickel and 2\u2010HEMA.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>This study confirms shared ACD imprinting among different allergens and shared pathways' predominant role in ACD elicitation in patients without atopic dermatitis, alongside allergen\u2010specific immune processes and mixed effector responses (type 1, 3 and 2).<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/all.16642","type":"journal-article","created":{"date-parts":[[2025,7,9]],"date-time":"2025-07-09T14:29:46Z","timestamp":1752071386000},"page":"3043-3054","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Transcriptomic Analysis of Allergic Patch Test Reactions in Non\u2010Atopic Patients: A Comparative Study Across Multiple Allergens"],"prefix":"10.1111","volume":"80","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5821-9780","authenticated-orcid":false,"given":"David","family":"Pesqu\u00e9","sequence":"first","affiliation":[{"name":"Department of Medicine Universitat Aut\u00f2noma de Barcelona (UAB)  Barcelona Spain"},{"name":"Department of Dermatology Hospital del Mar Research Institute  Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2166-6760","authenticated-orcid":false,"given":"Evelyn","family":"Andrades","sequence":"additional","affiliation":[{"name":"Department of Dermatology Hospital del Mar Research Institute  Barcelona Spain"},{"name":"Department of Medicine Universitat Pompeu Fabra (UPF)  Barcelona 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