{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T04:42:51Z","timestamp":1782967371399,"version":"3.54.5"},"reference-count":29,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,7,9]],"date-time":"2025-07-09T00:00:00Z","timestamp":1752019200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>A novel device, Skin Prick Automated Test (SPAT), previously showed reduced variability and more consistent test results compared to conventional skin prick test (SPT) to identify allergic sensitisation. This study aimed to clinically validate the adjusted SPAT cut\u2010off in patients with confirmed birch or house dust mite (HDM) allergy.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Seventy\u2010five adults were included: 25 non\u2010allergic subjects (confirmed by lack of allergy history and negative SPT), 25 birch and 25 HDM allergic rhinitis patients (both confirmed by positive SPT and nasal allergen challenge [NAC]). All subjects received a conventional SPT and an automated SPT for\n                      <jats:italic>B ver<\/jats:italic>\n                      ,\n                      <jats:italic>D pter<\/jats:italic>\n                      ,\n                      <jats:italic>D far<\/jats:italic>\n                      and control solutions.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>A cut\u2010off of 4.2 and 4.1\u2009mm, respectively, resulted in the highest accuracy to detect birch or HDM allergy using SPAT. Referring to previous study results suggesting a reliable cut\u2010off value of 4.5\u2009mm, it was decided to maintain 4.5\u2009mm as SPAT cut\u2010off indicating allergic sensitisation. Accuracy did not significantly differ between SPAT (96% using  4.5\u2009mm) and conventional SPT (98% using  3.0\u2009mm) to detect HDM allergy or to detect birch pollen allergy (100% for SPAT and SPT). SPAT wheal measurements performed through a ruler on the forearm or through digital measurement on a composite image did not significantly differ for any of the patient groups analysed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>SPAT showed an equivalent accuracy to detect birch pollen or HDM allergy compared to conventional SPT, using the adjusted 4.5\u2009mm SPAT cut\u2010off in patients with confirmed allergic rhinitis. The SPAT web viewer can be used easily and effectively for digital wheal measurement on a composite image.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/all.16645","type":"journal-article","created":{"date-parts":[[2025,7,9]],"date-time":"2025-07-09T13:43:43Z","timestamp":1752068623000},"page":"3302-3309","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Validation of the Skin Prick Automated Test (\n                    <scp>SPAT<\/scp>\n                    ) Cut\u2010Off Value in Birch Pollen and House Dust Mite Allergic Rhinitis Patients"],"prefix":"10.1111","volume":"80","author":[{"given":"Sven F.","family":"Seys","sequence":"first","affiliation":[{"name":"Hippo Dx  Aarschot Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3198-1396","authenticated-orcid":false,"given":"Alina","family":"Gherasim","sequence":"additional","affiliation":[{"name":"Centre de Recherche Clinique ALYATEC  Strasbourg France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florian","family":"Odul","sequence":"additional","affiliation":[{"name":"Centre de Recherche Clinique ALYATEC  Strasbourg France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank","family":"Dietsch","sequence":"additional","affiliation":[{"name":"Centre de Recherche Clinique ALYATEC  Strasbourg France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0133-0100","authenticated-orcid":false,"given":"Zuzana","family":"Diamant","sequence":"additional","affiliation":[{"name":"Allergy and Clinical Immunology Research Group, Department of Microbiology, Immunology &amp; Transplantation KU Leuven  Leuven Belgium"},{"name":"Department of Clinical Pharmacy and Pharmacology Univ Groningen, Univ Med Ctr Groningen  Groningen the Netherlands"},{"name":"Department of Respiratory Medicine, First Faculty of Medicine Charles University and Thomayer Hospital  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1629-8468","authenticated-orcid":false,"given":"Philippe","family":"Gevaert","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology, Upper Airways Research Laboratory Ghent University  Ghent Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter W.","family":"Hellings","sequence":"additional","affiliation":[{"name":"Allergy and Clinical Immunology Research Group, Department of Microbiology, Immunology &amp; Transplantation KU Leuven  Leuven Belgium"},{"name":"Department of Otorhinolaryngology\u2010Head and Neck Surgery UZ Leuven  Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5336-5335","authenticated-orcid":false,"given":"Dirk","family":"Loeckx","sequence":"additional","affiliation":[{"name":"Hippo Dx  Aarschot Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Senne","family":"Gorris","sequence":"additional","affiliation":[{"name":"Hippo Dx  Aarschot Belgium"},{"name":"Department of Otorhinolaryngology AZ Herentals  Herentals Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fr\u00e9d\u00e9ric","family":"de Blay","sequence":"additional","affiliation":[{"name":"Centre de Recherche Clinique ALYATEC  Strasbourg France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,7,9]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.15889"},{"key":"e_1_2_8_3_1","unstructured":"\u201cDiagnosed Allergic Conditions in Children Aged 0\u201317\u2009Years: United States \u201d(2023) https:\/\/www.cdc.gov\/nchs\/products\/databriefs\/db459.htm."},{"key":"e_1_2_8_4_1","unstructured":"\u201cMore Than a Quarter of U.S. Adults and Children Have at Least One Allergy \u201d(2023) https:\/\/www.cdc.gov\/nchs\/pressroom\/nchs_press_releases\/2022\/20220126.htm."},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.13306"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/2045-7022-3-3"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13223-016-0126-0"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/clt2.12220"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.13416"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1183\/13993003.02782-2021"},{"key":"e_1_2_8_11_1","first-page":"551","article-title":"The Prick Test, a Recent Cutaneous Test for the Diagnosis of Allergic Disorders","volume":"71","author":"Ebruster H.","year":"1959","journal-title":"Wiener Klinische Wochenschrift"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.4103\/0378-6323.192956"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.15619"},{"key":"e_1_2_8_14_1","first-page":"216","article-title":"Skin Prick Automated Test Device Offers More Reliable Allergy Test Results Compared to a Manual Skin Prick Test","volume":"25","author":"Seys S. 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