{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T19:58:25Z","timestamp":1791921505759,"version":"4.3.4"},"reference-count":48,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,7,25]],"date-time":"2025-07-25T00:00:00Z","timestamp":1753401600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,7,25]],"date-time":"2025-07-25T00:00:00Z","timestamp":1753401600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82230078"],"award-info":[{"award-number":["82230078"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["91740120"],"award-info":[{"award-number":["91740120"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22077097"],"award-info":[{"award-number":["22077097"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["21572173"],"award-info":[{"award-number":["21572173"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","award":["2042022dx0003"],"award-info":[{"award-number":["2042022dx0003"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","award":["2042023kf1011"],"award-info":[{"award-number":["2042023kf1011"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFA1303500"],"award-info":[{"award-number":["2022YFA1303500"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2018YFA0507603"],"award-info":[{"award-number":["2018YFA0507603"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2026,5]]},"abstract":"ABSTRACT<\/jats:title>\n \n Background<\/jats:title>\n \n Type 2 inflammation has emerged as a pivotal mechanism for asthma, which involves both innate and adaptive immunity. Human ficolin (\n FCN<\/jats:styled-content>\n )\u20102 (L\u2010ficolin,\n P35<\/jats:styled-content>\n ) and its mouse homolog\n FCN<\/jats:styled-content>\n \u2010A are one of the major pattern recognition molecules of plasma\/serum, acting as important initiators of the lectin complement system and playing important roles in immunity, including respiratory immunity. However, little is known about the role of\n FCN<\/jats:styled-content>\n \u20102\/A in allergic asthma.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n Serum\n FCN<\/jats:styled-content>\n \u20102 and\n IgE<\/jats:styled-content>\n levels in 90 allergic asthmatic patients and 48 healthy controls were measured by\n ELISA<\/jats:styled-content>\n . Aeroallergen house dust mite (\n HDM<\/jats:styled-content>\n )\u2010induced mouse model of asthma was generated in both wild type (\n WT<\/jats:styled-content>\n ) and\n FCN<\/jats:styled-content>\n \u2010A knockout (\n KO<\/jats:styled-content>\n ) mice. Mouse serum and bronchoalveolar lavage fluid (\n BALF<\/jats:styled-content>\n )\n IgE<\/jats:styled-content>\n levels, lung innate lymphoid cells (\n ILC<\/jats:styled-content>\n )1\/2\/3, the expression of transcription factors\n GATA3<\/jats:styled-content>\n , T\u2010bet, and\n ROR\u03b3t<\/jats:styled-content>\n , and the concentrations of type 2 cytokines in serum and\n BALF<\/jats:styled-content>\n were measured by\n FCM<\/jats:styled-content>\n ,\n RT<\/jats:styled-content>\n \u2010\n qPCR<\/jats:styled-content>\n , Western blot, and\n ELISA<\/jats:styled-content>\n .\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Serum\n FCN<\/jats:styled-content>\n \u20102 concentrations in patients with allergic asthma were significantly lower than those in healthy controls. Similarly, lower serum and\n BALF FCN<\/jats:styled-content>\n \u2010A concentrations were observed in\n HDM<\/jats:styled-content>\n \u2010induced asthma mouse models compared to those of uninduced mice. In the asthma mouse model,\n FCN<\/jats:styled-content>\n \u2010A\n KO<\/jats:styled-content>\n asthmatic mice had higher levels of total\n IgE<\/jats:styled-content>\n and\n HDM<\/jats:styled-content>\n \u2010specific\n IgE<\/jats:styled-content>\n (\n sIgE<\/jats:styled-content>\n ), \u03b2\u2010hexosaminidase (\u03b2\u2010\n HEX<\/jats:styled-content>\n ) and histamine secretion, as well as increased airway epithelial permeability with the release of\n FITC<\/jats:styled-content>\n \u2010dextran in sera, inflammatory cell infiltration and eosinophil counts, and displayed more severe disease symptoms with histological damage compared to\n WT<\/jats:styled-content>\n asthmatic mice.\n FCN<\/jats:styled-content>\n \u2010A\n KO<\/jats:styled-content>\n asthmatic mice showed decreased T\u2010bet\n +<\/jats:sup>\n ILC1<\/jats:styled-content>\n and increased\n IL<\/jats:styled-content>\n \u20105\n +<\/jats:sup>\n \/\n IL<\/jats:styled-content>\n \u201013\n +<\/jats:sup>\n ILC2<\/jats:styled-content>\n \/\n ILC2<\/jats:styled-content>\n proportions, p\u2010\n GATA3<\/jats:styled-content>\n expression, serum and\n BALF<\/jats:styled-content>\n type 2 cytokines\n IL<\/jats:styled-content>\n \u20104,\n IL<\/jats:styled-content>\n \u20105, and\n IL<\/jats:styled-content>\n \u201013, Th17 cytokine\n IL<\/jats:styled-content>\n \u201017, and chemokines\n CCL2<\/jats:styled-content>\n \/4 production. Importantly, the administration of exogenous\n FCN<\/jats:styled-content>\n \u2010A protected against mouse allergic airway inflammation with decreased\n ILC2<\/jats:styled-content>\n proportions and type 2 cytokines expression, serum total and allergen\u2010specific\n IgE<\/jats:styled-content>\n production. These results suggest that\n FCN<\/jats:styled-content>\n \u2010A suppresses both\n ILC2<\/jats:styled-content>\n innate immunity and\n IgE<\/jats:styled-content>\n \u2010mediated adaptive immunity during asthma.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n Our findings provide previously unreported evidence that\n FCN<\/jats:styled-content>\n \u2010A protects against allergic asthma by suppressing lung\n ILC2<\/jats:styled-content>\n \u2010driven type 2 inflammation.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/all.16661","type":"journal-article","created":{"date-parts":[[2025,7,25]],"date-time":"2025-07-25T07:25:01Z","timestamp":1753428301000},"page":"1587-1604","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Ficolin\u2010A Protects Against Allergic Asthma via Suppressing\n ILC2<\/scp>\n \u2010Drived Type 2 Inflammation"],"prefix":"10.1111","volume":"81","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-9569-3613","authenticated-orcid":false,"given":"Yu\u2010ke","family":"Xie","sequence":"first","affiliation":[{"name":"Hubei Province Key Laboratory of Allergy and Immunology, Department of Allergy Zhongnan Hospital of Wuhan University Wuhan China"},{"name":"Department of Immunology School of Basic Medical Sciences, State Key Laboratory of Virology, Frontier Science Center for Immunology and Metabolism, Wuhan University Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shan\u2010shan","family":"Xu","sequence":"additional","affiliation":[{"name":"Hubei Province Key Laboratory of Allergy and Immunology, Department of Allergy Zhongnan Hospital of Wuhan University Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong\u2010shuai","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Immunology School of Basic Medical Sciences, State Key Laboratory of Virology, Frontier Science Center for Immunology and Metabolism, Wuhan University Wuhan China"},{"name":"The Sixth People's Hospital of Zhengzhou Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiarong","family":"Li","sequence":"additional","affiliation":[{"name":"Hubei Province Key Laboratory of Allergy and Immunology, Department of Allergy Zhongnan Hospital of Wuhan University Wuhan China"},{"name":"Department of Immunology School of Basic Medical Sciences, State Key Laboratory of Virology, Frontier Science Center for Immunology and Metabolism, Wuhan University Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shu\u2010chen","family":"Zhang","sequence":"additional","affiliation":[{"name":"Hubei Province Key Laboratory of Allergy and Immunology, Department of Allergy Zhongnan Hospital of Wuhan University Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Xie","sequence":"additional","affiliation":[{"name":"Hubei Province Key Laboratory of Allergy and Immunology, Department of Allergy Zhongnan Hospital of Wuhan University Wuhan China"},{"name":"Department of Immunology School of Basic Medical Sciences, State Key Laboratory of Virology, Frontier Science Center for Immunology and Metabolism, Wuhan University Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1251-7608","authenticated-orcid":false,"given":"Ya\u2010dong","family":"Gao","sequence":"additional","affiliation":[{"name":"Hubei Province Key Laboratory of Allergy and Immunology, Department of Allergy Zhongnan Hospital of Wuhan University Wuhan China"},{"name":"Department of Allergy The First Affiliated Hospital, Zhejiang University School of Medicine Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8283-9381","authenticated-orcid":false,"given":"Xiao\u2010Lian","family":"Zhang","sequence":"additional","affiliation":[{"name":"Hubei Province Key Laboratory of Allergy and Immunology, Department of Allergy Zhongnan Hospital of Wuhan University Wuhan China"},{"name":"Department of Immunology School of Basic Medical Sciences, State Key Laboratory of Virology, Frontier Science Center for Immunology and Metabolism, Wuhan University Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,7,25]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1586\/ers.11.34"},{"issue":"1","key":"e_1_2_10_3_1","first-page":"S2","article-title":"Asthma: Definitions and Pathophysiology","volume":"5","author":"Mims J. W.","year":"2015","journal-title":"International Forum of Allergy and Rhinology"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.4168\/aair.2021.13.3.363"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/imr.12540"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-021-03830-7"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2021.12.783"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/imr.12706"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/ahg.12129"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1136\/archdischild-2021-322114"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2021.105462"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imlet.2020.11.006"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clim.2017.08.012"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0073859"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1302563"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.714511"},{"issue":"9","key":"e_1_2_10_17_1","first-page":"636","article-title":"Chinese Guidelines for the Diagnosis and Treatment of Allergic Asthma","volume":"58","author":"Chuntao L.","year":"2019","journal-title":"Zhonghua Nei Ke Za Zhi"},{"issue":"2","key":"e_1_2_10_18_1","first-page":"106","article-title":"Chinese Guideline for Diagnosis and Treatment of Allergic Rhinitis (2022, Revision)","volume":"57","author":"Subspecialty Group of Rhinology","year":"2022","journal-title":"Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1586\/14737159.4.3.273"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.4168\/aair.2021.13.2.177"},{"issue":"2","key":"e_1_2_10_21_1","first-page":"84","article-title":"Pulmonary Function Tests","volume":"80","author":"Ranu H.","year":"2011","journal-title":"Ulster Medical Journal"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1159\/000335670"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/imm.12741"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-021-04055-2"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/cmi.2009.32"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12250-016-3808-3"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12890-023-02772-2"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-022-05395-5"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-018-0412-5"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.01145"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2020.08.051"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2009.08.028"},{"issue":"2","key":"e_1_2_10_33_1","first-page":"399","article-title":"Pattern\u2010Recognition Molecule, Ficolin","volume":"63","author":"Endo Y.","year":"2008","journal-title":"Nihon Saikingaku Zasshi Japanese Journal of Bacteriology"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-0716-1016-9_12"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imbio.2006.11.014"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imbio.2006.11.007"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.14318"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.15601"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cytogfr.2018.12.001"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciimmunol.aaw2938"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201405-0859PP"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.13789"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41590-021-01067-0"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-021-00538-7"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.15240"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.14421"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2011.05.038"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0231865"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.4161\/tisb.24333"}],"container-title":["Allergy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/all.16661","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/all.16661","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/all.16661","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T12:10:49Z","timestamp":1778155849000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/all.16661"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,25]]},"references-count":48,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,5]]}},"alternative-id":["10.1111\/all.16661"],"URL":"https:\/\/doi.org\/10.1111\/all.16661","archive":["Portico"],"relation":{},"ISSN":["0105-4538","1398-9995"],"issn-type":[{"value":"0105-4538","type":"print"},{"value":"1398-9995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,25]]},"assertion":[{"value":"2025-01-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-20","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-25","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}