{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T01:10:34Z","timestamp":1791421834176,"version":"4.3.3"},"reference-count":62,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,8,30]],"date-time":"2025-08-30T00:00:00Z","timestamp":1756512000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2025,8,30]],"date-time":"2025-08-30T00:00:00Z","timestamp":1756512000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","award":["23\u201075\u201030016"],"award-info":[{"award-number":["23\u201075\u201030016"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Asthma is a chronic respiratory disease comprising different pheno\u2010 and endotypes. Diagnostic tools for the identification of allergic versus non\u2010allergic asthma are needed for new precision medicine\u2010based treatments.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To determine IgE sensitization profiles to multiple micro\u2010arrayed allergen molecules in adult patients with asthma in the Austrian LEAD (Lung, hEart, sociAl, boDy) cohort; to compare IgE\u2010 and non\u2010IgE sensitized patients with asthma; and to define possible allergen\u2010specific immunotherapy concepts for sensitized patients.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Out of 893 patients with a history of asthma, patients with current asthma (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u2009436) were analyzed for IgE sensitizations to 110 micro\u2010arrayed molecules from airborne and food allergen sources and by skin prick testing (SPT) with 10 allergen extracts (English plantain, mugwort, ragweed, timothy grass, ash tree, mites, dog, cat,\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>Alternaria alternata<\/jats:italic>\n                      <\/jats:styled-content>\n                      , and Fagales mix). Clinical asthma\u2010related parameters were compared between patients with IgE sensitization to asthma allergen molecules and non\u2010IgE sensitized patients with asthma.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>IgE sensitization was detected in 73.2% of patients with asthma using 63 micro\u2010arrayed respiratory allergens. The most recognized respiratory outdoor allergen molecules were Bet v 1 (32.8%) and Ole e 1 (23.2%) followed by grass pollen, ragweed, and mugwort allergens. Fel d 1 was the most frequently recognized respiratory indoor allergen molecule (42.7%) followed by house dust mite and dog allergen molecules. Micro\u2010arrayed allergens allowed the identification of IgE reactivity profiles indicative of genuine sensitizations to different allergen sources. IgE\u2010sensitized patients were significantly younger than non\u2010IgE\u2010sensitized patients with asthma (median age 44 versus 58\u2009years). Patients sensitized to respiratory allergens showed significantly better lung function (FEV1, FVC and FEV\/FVC) and less dyspnea but more allergic bronchitis than non\u2010IgE\u2010sensitized patients with asthma. More IgE\u2010sensitized patients used antihistamines but fewer inhaled corticosteroids than non\u2010IgE\u2010sensitized patients with asthma. Interestingly, eosinophil counts were lower both in ICS\u2010treated as well as untreated sensitized patients than in non\u2010sensitized patients with asthma.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Molecular allergy diagnosis allowed the detection of genuine IgE sensitizations in adult patients with asthma; enabling stratification for precision medicine\u2010based forms of personalized treatments such as allergen\u2010based immunotherapy (AIT) and\/or administration of biological treatments in asthma.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/all.70017","type":"journal-article","created":{"date-parts":[[2025,8,30]],"date-time":"2025-08-30T08:50:57Z","timestamp":1756543857000},"page":"130-144","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Molecular\n                    <scp>IgE<\/scp>\n                    Sensitization Profiling With Micro\u2010Arrayed Allergen Molecules in Adult Patients With Asthma From the\n                    <scp>LEAD<\/scp>\n                    Cohort: A Precision Medicine Approach"],"prefix":"10.1111","volume":"81","author":[{"given":"Huey\u2010Jy","family":"Huang","sequence":"first","affiliation":[{"name":"Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology Medical University of Vienna  Vienna Austria"},{"name":"Center for Molecular Allergology Karl Landsteiner University of Health Sciences  Krems Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robab","family":"Breyer\u2010Kohansal","sequence":"additional","affiliation":[{"name":"Ludwig Boltzmann Institute for Lung Health  Vienna Austria"},{"name":"Department of Respiratory and Pulmonary Diseases, Clinic Hietzing Vienna Healthcare Group  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katarzyna","family":"Niespodziana","sequence":"additional","affiliation":[{"name":"Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology Medical University of Vienna  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-9398-9629","authenticated-orcid":false,"given":"Charmaine J. M.","family":"Lim","sequence":"additional","affiliation":[{"name":"Ludwig Boltzmann Institute for Lung Health  Vienna Austria"},{"name":"Sigmund Freud Private University Faculty of Medicine  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie\u2010Kathrin","family":"Breyer","sequence":"additional","affiliation":[{"name":"Ludwig Boltzmann Institute for Lung Health  Vienna Austria"},{"name":"Department of Respiratory and Pulmonary Diseases, Site Penzing of Clinic Ottakring Vienna Healthcare Group  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5944-3365","authenticated-orcid":false,"given":"Rudolf","family":"Valenta","sequence":"additional","affiliation":[{"name":"Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology Medical University of Vienna  Vienna Austria"},{"name":"Center for Molecular Allergology Karl 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