{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T06:28:15Z","timestamp":1788848895277,"version":"build-2803163510"},"reference-count":47,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:00:00Z","timestamp":1760140800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:00:00Z","timestamp":1760140800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Atopic dermatitis (AD) and asthma are observed as epidemiologically linked allergic comorbidities, both characterized by elevated systemic IgE levels and type 2 immunity. Basophils play a pivotal role in these responses by producing interleukin\u20104 (IL\u20104), which is essential for IgE synthesis and allergic inflammation. However, their specific impact on the progression from AD to asthma remains unclear.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We utilized an AD model in basophil\u2010deficient Mcpt8Cre mice and temporarily basophil\u2010depleted mice, where topical application of the vitamin D analog MC903 induced the alarmin TSLP, resulting in AD\u2010like symptoms. In addition, we topically applied ovalbumin (OVA) as a model allergen to trace the allergen\u2010specific immune response. We determined allergen\u2010specific antibody formation by analysis of the germinal center reaction and measured serum antibody concentrations and IgE loading of basophils in the spleen and lung. We further challenged mice sensitized via the skin in anaphylaxis and allergic lung inflammation models.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Our results demonstrate that basophils promote loss of skin barrier integrity, allergen\u2010specific IgE formation, and subsequent allergic responses. Basophil depletion selectively during sensitization significantly reduced IgE\u2010dependent anaphylaxis and lung inflammation. In challenged lungs, reduced inflammation and eosinophilia were accompanied by lower levels of chemokines CCL17 and CCL24, which attract Th2 cells and eosinophils, respectively. Notably,\n                      <jats:italic>Il4<\/jats:italic>\n                      and\n                      <jats:italic>Il13<\/jats:italic>\n                      were not affected by basophil depletion during sensitization but were reduced in mice that permanently lack basophils.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>We find that basophils promote IgE formation and lung sensitization to skin\u2010encountered allergens and drive secondary allergen\u2010induced lung inflammation. We separate the role of basophils in sensitization from their effector function during anaphylaxis or lung inflammation relevant to envision novel strategies to prevent the development of allergic comorbidities.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/all.70093","type":"journal-article","created":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T09:40:55Z","timestamp":1760175655000},"page":"220-231","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Basophils in Skin\u2010Mediated Sensitization Drive Subsequent Lung Inflammation in Airway\u2010Challenged Mice"],"prefix":"10.1111","volume":"81","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-9941-4578","authenticated-orcid":false,"given":"E Da","family":"Choi","sequence":"first","affiliation":[{"name":"Department of Infection Biology University Hospital Erlangen and Friedrich\u2010Alexander University Erlangen\u2010Nuremberg (FAU)  Erlangen 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