{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T13:10:10Z","timestamp":1778159410206,"version":"3.51.4"},"reference-count":59,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T00:00:00Z","timestamp":1761523200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T00:00:00Z","timestamp":1761523200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>IgG4\u2010related chronic rhinosinusitis (IgG4\u2010CRS) is a new clinical entity characterized by nasal lesions; however, its pathogenesis remains unclear. This study aimed to reveal single\u2010cell transcriptomic changes in patients with IgG4\u2010CRS via single\u2010cell RNA sequencing (scRNA\u2010seq) and illustrate its pathogenesis at the single\u2010cell level.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Nasal mucosal tissues from five patients with IgG4\u2010CRS were used for unbiased scRNA\u2010seq. Bioinformatic analysis was performed using these five samples and three published control samples. Immunohistochemical and multicolor immunofluorescence analyses were performed to validate the sequencing results.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>A total of 49,063 cells and 11 sub\u2010clusters were identified. CXCL12 secretion increased in endothelial cells and fibroblasts, and CXCR4 was upregulated in the immune cells of IgG4\u2010CRS. Enhanced chemotaxis mediated by the CXCL12\u2010CXCR4 axis recruits excess immune cells and overactivation. Compared with those in the control group, the immunocompetence of CD4+ T cells and cytotoxicity of CD8+ T cells were enhanced in the IgG4\u2010CRS group, and B cells were more differentiated to secrete IgG\u2010type plasma cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Chemotaxis of the CXCL12\u2010CXCR4 axis plays a role in the pathogenesis of IgG4\u2010CRS by influencing both the immune and nonimmune cells of IgG4\u2010CRS.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/all.70124","type":"journal-article","created":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T13:27:24Z","timestamp":1761658044000},"page":"1635-1649","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["The CXCL12\u2010CXCR4 Axis Mediates Lymphocyte Immune Overactivation in IgG4\u2010Related Chronic Rhinosinusitis"],"prefix":"10.1111","volume":"81","author":[{"given":"Jing","family":"Ding","sequence":"first","affiliation":[{"name":"Department of Pathology Beijing Tongren Hospital, Capital Medical University  Beijing China"},{"name":"Beijing Key Laboratory of Head and Neck Molecular Pathological Diagnosis Beijing Tongren Hospital, Capital Medical University  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Cui","sequence":"additional","affiliation":[{"name":"Department of Rheumatology and Immunology Beijing Tongren Hospital, Capital Medical University  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0646-5135","authenticated-orcid":false,"given":"Chengshuo","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Head and Neck Surgery Beijing Tongren Hospital, Capital Medical University  Beijing China"},{"name":"Beijing Institute of Otolaryngology, Beijing Laboratory of Allergic Diseases, Beijing Key Laboratory of Nasal Diseases, Key Laboratory of Otolaryngology Head and Neck Surgery, Ministry of Education Capital Medical University  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ming","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Pathology Beijing Tongren Hospital, Capital Medical University  Beijing China"},{"name":"Beijing Key Laboratory of Head and Neck Molecular Pathological Diagnosis Beijing Tongren Hospital, Capital Medical University  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1080-4267","authenticated-orcid":false,"given":"Yuan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Allergy Beijing TongRen Hospital, Capital Medical University  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0910-9884","authenticated-orcid":false,"given":"Luo","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Head and Neck Surgery Beijing Tongren Hospital, Capital Medical University  Beijing China"},{"name":"Beijing Institute of Otolaryngology, Beijing Laboratory of Allergic Diseases, Beijing Key Laboratory of Nasal Diseases, Key Laboratory of Otolaryngology Head and Neck Surgery, Ministry of Education Capital Medical University  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yingshi","family":"Piao","sequence":"additional","affiliation":[{"name":"Department of Pathology Beijing Tongren Hospital, Capital Medical University  Beijing China"},{"name":"Beijing Key Laboratory of Head and Neck Molecular Pathological Diagnosis Beijing Tongren Hospital, Capital Medical University  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,10,27]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.3109\/00016489.2010.533699"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/his.12775"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2017.12.011"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.advms.2017.04.001"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-15398-x"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anl.2023.10.006"},{"issue":"2","key":"e_1_2_11_8_1","first-page":"85","article-title":"Immunoglobulin G4\u2010Related Sclerosing Disease of the Paranasal Sinuses: A Case Report and Literature Review","volume":"7","author":"Vandjelovic N. 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