{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T22:23:37Z","timestamp":1787783017626,"version":"build-2784847793"},"reference-count":68,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T00:00:00Z","timestamp":1771286400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T00:00:00Z","timestamp":1771286400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100011102","name":"Seventh Framework Programme","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100011102","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Atopic dermatitis (AD) is a chronic inflammatory skin disease characterized by immune dysregulation and epithelial barrier dysfunction. Although transcriptional changes in AD skin are increasingly well characterized, DNA methylation patterns remain less well understood.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Here, we present an integrated analysis of matched DNA methylomes, transcriptomes, and microbiomes from lesional (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200940), adjacent non\u2010lesional (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200938), and healthy control (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200940) skin, using complementary cell\u2010type\u2010adjusted models.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      We identified thousands of differentially methylated regions (DMRs) across all pairwise contrasts (lesional vs. healthy:\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200913,514; lesional vs. non\u2010lesional:\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20094591; non\u2010lesional vs. healthy:\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20091716), including both hyper\u2010 and hypo\u2010methylated regions with balanced effect sizes. A core subset of DMRs persisted after methylation\u2010based adjustment, whereas the extensive lesional vs. non\u2010lesional set was largely composition\u2010driven. Integration with transcriptomic co\u2010expression networks linked DMRs to immune\u2013epithelial modules, and 225 DMR\u2013gene pairs showed significant anti\u2010correlation (FDR &lt;\u20090.05). Lesional skin with dominant\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>Staphylococcus aureus<\/jats:italic>\n                      <\/jats:styled-content>\n                      colonization differed at 92 DMRs compared with absent\u2010colonized skin, of which 70 also overlapped with local severity. Pathway analyses consistently highlighted Rho GTPase and actin\u2013junctional programs across analytic layers, suggesting that Rho GTPase signaling is a central integrator of immune, epithelial, and microbial interactions in AD.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Our study underscores the importance of epigenomic remodeling in AD and highlights potential avenues for precision intervention in chronic inflammatory skin disease.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/all.70259","type":"journal-article","created":{"date-parts":[[2026,2,18]],"date-time":"2026-02-18T06:39:46Z","timestamp":1771396786000},"page":"2417-2434","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Integrated Methylome\u2013Transcriptome Analysis Reveals Epigenomic Remodeling and Rho\n                    <scp>GTPase<\/scp>\n                    \u2013Linked Immune\u2013Epithelial Crosstalk in Atopic Dermatitis"],"prefix":"10.1111","volume":"81","author":[{"given":"Tiina","family":"Skoog","sequence":"first","affiliation":[{"name":"Department of Medicine, Huddinge Karolinska Institutet  Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-3839-6859","authenticated-orcid":false,"given":"Ying","family":"Yang","sequence":"additional","affiliation":[{"name":"Institute of Environmental Medicine Karolinska Institutet  Stockholm Sweden"},{"name":"Medical Faculty, Human Microbiome Research Program (HUMI) University of Helsinki  Helsinki Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3184-8376","authenticated-orcid":false,"given":"Peter","family":"Olah","sequence":"additional","affiliation":[{"name":"Medical Faculty, Department of Dermatology, University Hospital 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