{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T18:06:00Z","timestamp":1790877960257,"version":"4.1.0"},"reference-count":45,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T00:00:00Z","timestamp":1771459200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T00:00:00Z","timestamp":1771459200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Immediate drug allergic reactions (IDAR) to betalactams are frequent, yet mislabelling remains common and negatively impacts clinical decisions. Conventional diagnostics such as STs and drug provocation are effective but limited by time, risk, and contraindications in severe cases. In\u00a0vitro alternatives\u2014sIgEquantification and basophil activation tests (BAT)\u2014offer safer options, although performance may be affected by biological variability and suboptimal sensitivity with an important drawback for the latter in patients with non\u2010releaser basophils. This study aimed to evaluate a mast cell activation test (MAT) based on human CD34\n                      <jats:sup>+<\/jats:sup>\n                      \u2010derived mast cells (dMCs) for IDAR diagnosis to amoxicillin (AX) using both free AX and dendrimeric amoxicilloyl conjugates (G4\/G5\u2010AXO).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      CD34\n                      <jats:sup>+<\/jats:sup>\n                      cells were cultured for 10\u201312\u2009weeks to generate dMCs. After passive sensitization with sera from AX\u2010allergic patients (\n                      <jats:italic>N<\/jats:italic>\n                      \u2009=\u200928) or tolerant controls (\n                      <jats:italic>N<\/jats:italic>\n                      \u2009=\u200911), dMCs were stimulated with free AX, G4\u2010AXO, or G5\u2010AXO. Activation was quantified by CD63 expression via flow cytometry. Diagnostic performance was compared with BAT and sIgE determination by ImmunoCAP.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>MAT with free AX achieved 53.57% sensitivity, G4\/G5\u2010AXO reached 46.43%, and all methods maintained 100% specificity. Combining results with AX or dendrimeric stimuli increased sensitivity to 75% while preserving specificity. Importantly, MAT identified positive cases among BAT non\u2010releasers and patients with undetectable sIgE.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Under optimized conditions, MAT using dMCs and different AX determinants reached 75% sensitivity and 100% specificity. The complementary use of free AX and dendrimeric conjugates expands detection across heterogeneous IgE reactivity profiles from AX\u2010allergic patients, reinforcing the diagnostic value of advanced cellular models and engineered allergens.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/all.70267","type":"journal-article","created":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T12:12:09Z","timestamp":1771503129000},"page":"2910-2922","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Successful Application of the Mast Cell Activation Test in Immediate Hypersensitivity to Amoxicillin"],"prefix":"10.1111","volume":"81","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3318-3689","authenticated-orcid":false,"given":"Jose A.","family":"C\u00e9spedes","sequence":"first","affiliation":[{"name":"Allergy Research Group IBIMA Plataforma BIONAND  M\u00e1laga Spain"},{"name":"Department of Medicine and Dermatology, Faculty of Medicine Universidad de M\u00e1laga  M\u00e1laga Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clara","family":"Lebr\u00f3n\u2010Mart\u00edn","sequence":"additional","affiliation":[{"name":"Allergy Research Group IBIMA Plataforma BIONAND  M\u00e1laga Spain"},{"name":"Department of Medicine and Dermatology, Faculty of Medicine Universidad de M\u00e1laga  M\u00e1laga Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luc\u00eda","family":"Vallecillos\u2010Azor","sequence":"additional","affiliation":[{"name":"Allergy Research Group IBIMA Plataforma BIONAND  M\u00e1laga Spain"},{"name":"Department of Medicine and Dermatology, Faculty of Medicine Universidad de M\u00e1laga  M\u00e1laga Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pablo","family":"Torres","sequence":"additional","affiliation":[{"name":"Allergy Research Group IBIMA Plataforma BIONAND  M\u00e1laga Spain"},{"name":"Department of 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