{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T11:38:18Z","timestamp":1784547498743,"version":"3.55.0"},"reference-count":25,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T00:00:00Z","timestamp":1772668800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T00:00:00Z","timestamp":1772668800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82373476"],"award-info":[{"award-number":["82373476"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82173445"],"award-info":[{"award-number":["82173445"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Atopic dermatitis (AD) patients exhibit a paradox of impaired skin barrier with intense itching, yet often demonstrate rapid re\u2010epithelialization after scratching. While basophils are key effector cells in allergic inflammation, their role in the subsequent tissue repair remains unexplored.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>We investigated whether basophils, recruited during sensitized skin responses, contribute to wound healing.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Using single\u2010cell RNA sequencing, flow cytometry, and immunofluorescence, we mapped basophil infiltration and activation in sensitizer (oxazolone)\u2010induced skin injury models. We employed genetic (\n                      <jats:italic>Mcpt8<\/jats:italic>\n                      <jats:sup>\n                        <jats:italic>CT\/+<\/jats:italic>\n                      <\/jats:sup>\n                      <jats:italic>R26<\/jats:italic>\n                      <jats:sup>\n                        <jats:italic>DTA\/+<\/jats:italic>\n                      <\/jats:sup>\n                      ) and antibody\u2010mediated (anti\u2010Fc\u03b5RI) basophil depletion, as well as basophil\u2010specific\n                      <jats:italic>Il4\/Il13<\/jats:italic>\n                      knockout mice (\n                      <jats:italic>Il4\/13<\/jats:italic>\n                      <jats:sup>fl\/fl<\/jats:sup>\n                      <jats:italic>Mcpt8<\/jats:italic>\n                      <jats:sup>CT\/+<\/jats:sup>\n                      ) to define their functional contribution. Macrophage polarization was assessed by flow cytometry, RT\u2010qPCR, and immunohistochemistry.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      We identified a significant enrichment of basophils in wounded skin, peaking at day 3\u20136 post\u2010injury. Sensitizer\u2010challenge enhanced basophil recruitment and accelerated wound closure, angiogenesis, and re\u2010epithelialization. Depletion of basophils severely impaired these repair processes. Mechanistically, basophils were the predominant source of IL\u20104 and IL\u201013 in the early wound microenvironment. These cytokines were essential for driving macrophage polarization toward a pro\u2010repair M2 phenotype. Loss of\n                      <jats:italic>IL4\/IL13<\/jats:italic>\n                      specifically in basophils phenocopied the healing defects observed in basophil\u2010deficient mice, and this could be rescued by local cytokine administration.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Our study uncovers a novel pro\u2010repair function of basophils in sensitizer\u2010exposed skin. Beyond their well\u2010known role in provoking itch and inflammation, basophils are critical for initiating type 2 immune\u2010mediated tissue regeneration via IL\u20104\/IL\u201013\u2010dependent macrophage reprogramming. This axis represents a promising therapeutic target for chronic wounds, particularly in the context of allergic skin disorders.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/all.70279","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T17:55:52Z","timestamp":1772992552000},"page":"1487-1499","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Sensitizer\u2010Induced Basophils Accelerate Skin Re\u2010Epithelialization via\n                    <scp>IL<\/scp>\n                    \u20104\/\n                    <scp>IL<\/scp>\n                    \u201013\u2010Mediated Macrophage Polarization"],"prefix":"10.1111","volume":"81","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9127-5565","authenticated-orcid":false,"given":"Yufei","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Dermatology The Second Affiliated Hospital of Xi'an Jiaotong University  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1762-0532","authenticated-orcid":false,"given":"Xueting","family":"Peng","sequence":"additional","affiliation":[{"name":"Department of Dermatology The Second Affiliated Hospital of Xi'an Jiaotong University  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7312-2816","authenticated-orcid":false,"given":"Kaixuan","family":"Ren","sequence":"additional","affiliation":[{"name":"Department of Dermatology The Second Affiliated Hospital of Xi'an Jiaotong University  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-2218-2082","authenticated-orcid":false,"given":"Shiran","family":"Kang","sequence":"additional","affiliation":[{"name":"Department of Dermatology The Second Affiliated Hospital of Xi'an Jiaotong University  Xi'an 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