{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T18:31:47Z","timestamp":1786559507349,"version":"build-2736575974"},"reference-count":44,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T00:00:00Z","timestamp":1773360000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T00:00:00Z","timestamp":1773360000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Tape\u2010strip RNA sequencing enables non\u2010invasive molecular profiling of inflammatory skin diseases. However, its ability to capture immune activity, disease severity, and treatment response in alopecia areata (AA)\u2014a follicle\u2010centered autoimmune disorder\u2014has not been systematically evaluated in relation to follicular immune activity and treatment response.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To determine whether scalp tape\u2010strip RNA sequencing captures immune pathways associated with disease severity and treatment response in AA, and to assess its concordance with matched scalp biopsies.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We analyzed 61 RNA\u2010seq profiles, including 46 scalp tape\u2010strip samples (19 healthy controls, 17 active AA, and 10 post\u2010baricitinib samples\u2014five responders and five non\u2010responders) and 15 lesional scalp biopsies. Nine tape\u2010strip\u2013biopsy pairs were obtained from the same scalp region. Batch\u2010corrected expression data were analyzed using covariate\u2010adjusted linear models and gene set\u2013based enrichment analyses interrogating immune, cytotoxic, and follicular epithelial programs.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Tape\u2010strip RNA sequencing robustly recapitulated established AA\u2010associated immune signatures, including activation of interferon\/JAK\u2013STAT signaling and cytotoxic T\/NK cell pathways, together with suppression of follicular epithelial programs. Increasing disease severity was associated with progressive immune activation and epithelial loss. Baricitinib responders showed partial normalization of inflammatory signatures, whereas non\u2010responders retained persistent immune activation, consistent with molecular non\u2010response. Covariate\u2010adjusted models identified treatment response\u2013specific transcriptional changes. Tape\u2010strip and biopsy transcriptomes demonstrated high concordance.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Scalp tape\u2010strip RNA sequencing captures clinically relevant immune and treatment\u2010responsive molecular signatures in alopecia areata and represents a promising platform for longitudinal immune monitoring and biomarker\u2010driven stratification of treatment response in follicle\u2010centered autoimmune disease.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/all.70293","type":"journal-article","created":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T16:36:05Z","timestamp":1773419765000},"page":"2923-2932","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Non\u2010Invasive Scalp Tape\u2010Strip\n                    <scp>RNA<\/scp>\n                    Sequencing Captures Disease Activity and Treatment\u2010Response Signatures in Alopecia Areata"],"prefix":"10.1111","volume":"81","author":[{"given":"Carmen","family":"Moch\u00f3n\u2010Jim\u00e9nez","sequence":"first","affiliation":[{"name":"Inflammatory Immune\u2010Mediated Chronic Skin Diseases Laboratory IMIBIC\/University of C\u00f3rdoba  C\u00f3rdoba Spain"},{"name":"Department of Dermatology Reina Sof\u00eda University Hospital  C\u00f3rdoba Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7223-1922","authenticated-orcid":false,"given":"Jes\u00fas","family":"Gay\u2010Mimbrera","sequence":"additional","affiliation":[{"name":"Inflammatory Immune\u2010Mediated Chronic Skin Diseases Laboratory IMIBIC\/University of C\u00f3rdoba  C\u00f3rdoba Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viviana","family":"D\u00e1vila\u2010Flores","sequence":"additional","affiliation":[{"name":"Department of Pathology Reina Sof\u00eda University Hospital  C\u00f3rdoba Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helen","family":"He","sequence":"additional","affiliation":[{"name":"Department of Dermatology Icahn School of Medicine at Mount Sinai  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jerry","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Dermatology Icahn School of Medicine at Mount Sinai  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irene","family":"Rivera\u2010Ruiz","sequence":"additional","affiliation":[{"name":"Inflammatory Immune\u2010Mediated Chronic Skin Diseases Laboratory IMIBIC\/University of C\u00f3rdoba  C\u00f3rdoba Spain"},{"name":"Department of Dermatology Reina Sof\u00eda University Hospital  C\u00f3rdoba Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pedro J.","family":"G\u00f3mez\u2010Arias","sequence":"additional","affiliation":[{"name":"Inflammatory Immune\u2010Mediated Chronic Skin Diseases Laboratory IMIBIC\/University of C\u00f3rdoba  C\u00f3rdoba Spain"},{"name":"Department of Dermatology Reina Sof\u00eda University Hospital  C\u00f3rdoba Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan de","family":"Luque\u2010Fern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Inflammatory Immune\u2010Mediated Chronic Skin Diseases Laboratory IMIBIC\/University of C\u00f3rdoba  C\u00f3rdoba Spain"},{"name":"Department of Dermatology Reina Sof\u00eda University Hospital  C\u00f3rdoba Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0882-8163","authenticated-orcid":false,"given":"Benjamin","family":"Ungar","sequence":"additional","affiliation":[{"name":"Department of Dermatology Icahn School of Medicine at Mount Sinai  New York New York 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