{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T13:04:59Z","timestamp":1779368699863,"version":"3.53.0"},"reference-count":60,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T00:00:00Z","timestamp":1779321600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T00:00:00Z","timestamp":1779321600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund","doi-asserted-by":"publisher","award":["F4615\u2010B19"],"award-info":[{"award-number":["F4615\u2010B19"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund","doi-asserted-by":"publisher","award":["P32953"],"award-info":[{"award-number":["P32953"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund","doi-asserted-by":"publisher","award":["I4437"],"award-info":[{"award-number":["I4437"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100020344","name":"Gesellschaft f\u00fcr Forschungsf\u00f6rderung Nieder\u00f6sterreich","doi-asserted-by":"publisher","award":["Danube Allergy Research Cluster (DARC) 1.0"],"award-info":[{"award-number":["Danube Allergy Research Cluster (DARC) 1.0"]}],"id":[{"id":"10.13039\/100020344","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Allergy"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Sublingual allergen immunotherapy (SLIT) is an effective treatment for immunoglobulin (Ig)E\u2010mediated allergies. Its success is associated with allergen\u2010specific (s)IgG, which blocks IgE\u2010mediated mechanisms. Preventive effects of SLIT in children before allergy\u2010symptom onset remain largely unexplored.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A randomized trial was conducted between October 06, 2017 and December 15, 2022, which included house dust mite (HDM)\u2010sensitized preschool children (aged 3\u20135\u2009years) showing no allergy symptoms. They were randomized (2:2 blocks) to HDM\u2010SLIT (300 index of reactivity\/day, Staloral) or placebo solution for 2\u2009years. Children receiving\u2009&gt;\u20094\u2009months of treatment were included in the analysis. Primary objective of the study was to compare the groups for change in major HDM allergen\u2010Der p 1\u2010sIgG levels from baseline to end of treatment (EOT). Secondary objectives were to compare longitudinal changes (at 4 and 12\u2009months, and EOT) in the (i) levels of HDM\u2010sIgG subclasses and \u2010sIgE using ImmunoCAP\/\u2010ISAC and\/or ELISA (ii) Der p\u2010reactivity in skin and basophils using skin prick test and basophil activation test (BAT), (iii) sensitization status defined as sIgE\u2010positivity in skin and\/or serum, and (iv) Der p\u2010sIgE\u2010blocking activity using inhibition\u2010BAT.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Eighteen children received HDM\u2010SLIT (mean age\u2009=\u20094.26\u2009\u00b1\u20090.37\u2009years, eight females) and 15 placebo (mean age\u2009=\u20094.16\u2009\u00b1\u20090.59\u2009years, nine females). HDM\u2010SLIT increased Der p 1\u2010sIgG levels between baseline and EOT versus placebo (effect size\u2009=\u20093.11 [95% CL 1.36\u20134.82],\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.001). Additional changes induced by HDM\u2010SLIT were: increased levels of HDM\u2010sIgG\n                      <jats:sub>1<\/jats:sub>\n                      and \u2010sIgG\n                      <jats:sub>4<\/jats:sub>\n                      , no long\u2010term increase in HDM\u2010sIgE levels, reduced Der p\u2010reactivity in skin and basophils (\n                      <jats:italic>p<\/jats:italic>\n                      values\u2009\u2264\u20090.05), and blunted development of new sensitizations. Sera from HDM\u2010SLIT treated individuals displayed blocking activity on basophils (\n                      <jats:italic>p<\/jats:italic>\n                      values\u2009\u2264\u20090.05).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Preventive application of HDM\u2010SLIT in allergy\u2010prone children induces immunomodulatory effects early during treatment (elevated sIgG levels with blocking activity and reduced effector cell responses), thereby revealing potential to interfere with allergy development.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Trial Registration<\/jats:title>\n                    <jats:p>EudraCT#2016\u2010002844\u201018<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/all.70387","type":"journal-article","created":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T12:04:58Z","timestamp":1779365098000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Preventive Application of House Dust Mite\u2010Sublingual Immunotherapy Induces Blocking Antibodies in Sensitized Preschool Children"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-3831-1852","authenticated-orcid":false,"given":"Varsha","family":"Dwivedi","sequence":"first","affiliation":[{"name":"Division of Paediatric Pulmonology, Allergology and Endocrinology, Department of Paediatrics and Adolescent Medicine, Comprehensive Centre of Paediatrics Medical University of Vienna  Vienna 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Mia","family":"Gona\u2010H\u00f6pler","sequence":"additional","affiliation":[{"name":"Division of Paediatric Pulmonology, Allergology and Endocrinology, Department of Paediatrics and Adolescent Medicine, Comprehensive Centre of Paediatrics Medical University of Vienna  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina","family":"Bannert","sequence":"additional","affiliation":[{"name":"Division of Paediatric Pulmonology, Allergology and Endocrinology, Department of Paediatrics and Adolescent Medicine, Comprehensive Centre of Paediatrics Medical University of Vienna  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2596-6640","authenticated-orcid":false,"given":"Saskia","family":"Gruber","sequence":"additional","affiliation":[{"name":"Division of Paediatric Pulmonology, Allergology and Endocrinology, Department of Paediatrics and Adolescent Medicine, Comprehensive Centre of Paediatrics 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