{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T10:01:40Z","timestamp":1790676100905,"version":"4.1.0"},"reference-count":17,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T00:00:00Z","timestamp":1786492800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T00:00:00Z","timestamp":1786492800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003196","name":"Ministero della Salute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003196","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Allergy"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Hazelnut and walnut allergy may cause severe reactions, but the clinical relevance of individual seed storage proteins, including recently available vicilins, remains incompletely defined.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We performed an observational analysis of a consecutive tertiary\u2010care cohort of 114 patients with tree nut allergy, including 70 with walnut allergy and 44 with hazelnut allergy. Clinical severity was classified as mild, moderate, or severe. Specific IgE to selected hazelnut and walnut components was assessed by ImmunoCAP. We evaluated sensitisation frequencies, severity\u2010stratified molecular profiles, and the diagnostic contribution of newly available vicilins in a singleplex format, vicilin co\u2010sensitisation patterns by network and heatmap analyses, ROC performance for severe reactions, and parsimonious multivariable logistic regression models adjusted for age and sex.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Severe reactions were more frequent in hazelnut than in walnut allergy (43.2% vs. 24.3%). Cor a 14, Cor a 9, and Cor a 16 showed the clearest severity\u2010related gradients in hazelnut allergy, and Jug r 1 and Jug r 2 in walnut allergy. Co\u2010sensitisation was strongest between the type 2 vicilins Cor a 16 and Jug r 2. Cor a 16 showed the highest AUC among hazelnut components (0.72), with overlapping performance for Cor a 14 and Cor a 9, whereas Jug r 1 performed best among walnut components (AUC 0.89). After adjustment for age and sex, severe reactions remained associated with Cor a 14, Jug r 1, and Jug r 2.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>In this tertiary\u2010care cohort, individual seed storage proteins differed in their associations with reaction severity. Cor a 14 and Jug r 1 showed the strongest adjusted associations, while type 2 vicilins dominated co\u2010sensitisation patterns. Overall, these findings support the clinical value of molecule\u2010resolved diagnostics and suggest that integrating multiple vicilin components may improve molecular stratification of tree nut allergy, although external validation is required.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/all.70482","type":"journal-article","created":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T13:46:50Z","timestamp":1786542410000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Hazelnut and Walnut Vicilins in Tree Nut Allergy: Co\u2010Sensitization Patterns and Clinical Severity Associations"],"prefix":"10.1111","author":[{"given":"Danilo","family":"Villalta","sequence":"first","affiliation":[{"name":"S.C. di Immunologia e Allergologia di Laboratorio PO S. Maria Degli Angeli  Pordenone Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-0165-4863","authenticated-orcid":false,"given":"Giulia","family":"Marcassa","sequence":"additional","affiliation":[{"name":"S.C. di Immunologia e Allergologia di Laboratorio PO S. Maria Degli Angeli  Pordenone Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriella","family":"Cusa","sequence":"additional","affiliation":[{"name":"Fondazione Luigi Maria Monti, IDI\u2010IRCCS  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariaelisabetta","family":"Conte","sequence":"additional","affiliation":[{"name":"S.C. di Immunologia e Allergologia di Laboratorio PO S. Maria Degli Angeli  Pordenone Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Buzzulini","sequence":"additional","affiliation":[{"name":"S.C. di Immunologia e Allergologia di Laboratorio PO S. Maria Degli Angeli  Pordenone Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Pontoppidan","sequence":"additional","affiliation":[{"name":"Thermo Fisher Scientific  Uppsala Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisabetta","family":"Caprini","sequence":"additional","affiliation":[{"name":"Fondazione Luigi Maria Monti, IDI\u2010IRCCS  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonas","family":"\u00d6stling","sequence":"additional","affiliation":[{"name":"Thermo Fisher Scientific  Uppsala Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lars","family":"Mattsson","sequence":"additional","affiliation":[{"name":"Thermo Fisher Scientific  Uppsala Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie","family":"Holmqvist","sequence":"additional","affiliation":[{"name":"Thermo Fisher Scientific  Uppsala Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mauro","family":"Giani","sequence":"additional","affiliation":[{"name":"Fondazione Luigi Maria Monti, IDI\u2010IRCCS  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0658-2449","authenticated-orcid":false,"given":"Lorenzo","family":"Cecchi","sequence":"additional","affiliation":[{"name":"Allergy and Clinical Immunology Unit San Giovanni di Dio Hospital  Florence Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Riccardo","family":"Asero","sequence":"additional","affiliation":[{"name":"Ambulatorio di Allergologia Clinica San Carlo  Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7779-3411","authenticated-orcid":false,"given":"Jonas","family":"Lidholm","sequence":"additional","affiliation":[{"name":"Thermo Fisher Scientific  Uppsala Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9391-9168","authenticated-orcid":false,"given":"Enrico","family":"Scala","sequence":"additional","affiliation":[{"name":"Fondazione Luigi Maria Monti, IDI\u2010IRCCS  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,12]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.13501"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.18176\/jiaci.0713"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.23822\/EurAnnACI.1764-1489.54"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2008.04.014"},{"key":"e_1_2_11_6_1","first-page":"945","article-title":"Seed Storage Proteins: Structures and Biosynthesis","volume":"7","author":"Shewry P. 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