{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T17:06:37Z","timestamp":1787331997817,"version":"build-2736575974"},"reference-count":26,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2014,6,9]],"date-time":"2014-06-09T00:00:00Z","timestamp":1402272000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Anaesthesia"],"published-print":{"date-parts":[[2014,9]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>Malignant hyperthermia is a dreaded complication of general anaesthesia. Predisposed individuals can be identified using the standardised caffeine\/halothane in\u2010vitro contracture test on a surgically dissected skeletal muscle specimen. Skeletal muscle is composed of muscle fibres and interwoven fascial components. Several malignant hyperthermia\u2010associated neuromuscular diseases are associated with an altered connective tissue composition. We analysed adjacent fascial components of skeletal muscle histologically and physiologically. We investigated whether the fascial tissue is sensitive to electrical or pharmacological stimulation in a way similar to the in\u2010vitro contracture test for diagnosing malignant hyperthermia. Using immunohistochemical staining, \u03b1\u2010smooth muscle actin\u2010positive cells (myofibroblasts) were detected in the epi\u2010, endo\u2010 and perimysium of human fascial tissue. Force measurements on isolated fascial strips after pharmacological challenge with mepyramin revealed that myofascial tissue is actively regulated by myofibroblasts, thereby influencing the biomechanical properties of skeletal muscle. Absence of electrical reactivity and insensitivity to caffeine and halothane suggests that, reassuringly, the malignant hyperthermia diagnostic in\u2010vitro contracture test is not influenced by the muscular fascial tissue.<\/jats:p>","DOI":"10.1111\/anae.12752","type":"journal-article","created":{"date-parts":[[2014,6,9]],"date-time":"2014-06-09T07:04:49Z","timestamp":1402297489000},"page":"1002-1008","source":"Crossref","is-referenced-by-count":10,"title":["Contractile elements in muscular fascial tissue \u2013 implications for in\u2010vitro contracture testing for malignant hyperthermia"],"prefix":"10.1111","volume":"69","author":[{"given":"K.","family":"Hoppe","sequence":"first","affiliation":[{"name":"Department of Anaesthesiology Ulm University Ulm Germany"},{"name":"Department of Anaesthesia Intensive Care Medicine and Pain Therapy Frankfurt University Frankfurt am Main Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Schleip","sequence":"additional","affiliation":[{"name":"Division of Neurophysiology Ulm University Ulm Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Lehmann\u2010Horn","sequence":"additional","affiliation":[{"name":"Division of Neurophysiology Ulm University Ulm Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"J\u00e4ger","sequence":"additional","affiliation":[{"name":"Division of Neurophysiology Ulm University Ulm Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W.","family":"Klingler","sequence":"additional","affiliation":[{"name":"Division of Neurophysiology Ulm University Ulm Germany"},{"name":"Department of Neuroanaesthesiology Neurosurgical University Guenzburg 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