{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T18:21:28Z","timestamp":1776450088920,"version":"3.51.2"},"reference-count":30,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2014,11,27]],"date-time":"2014-11-27T00:00:00Z","timestamp":1417046400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Anaesthesia"],"published-print":{"date-parts":[[2015,2]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>Epidural blood patches may be used to treat post\u2010dural puncture headache following accidental dural puncture in parturients. Their mode of action and the optimum volume of blood for injection remain controversial, with the interaction between injected blood and cerebrospinal fluid unknown. We aimed to establish the effects of serial haemodilution of whole blood with cerebrospinal fluid from 34 pregnant patients compared with serial haemodilution with Hartmann's solution, using the thromboelastogram. Haemodilution with either cerebrospinal fluid or Hartmann's solution had significant procoagulant and clot destabilising effects, enhanced with progressive haemodilution up to 30%. The effect of cerebrospinal fluid was greater compared with Hartmann's solution (p\u00a0&lt;\u00a00.001). Cerebrospinal fluid led to a mean (95% CI) decrease in r\u2010time by 2.4 (1.6\u20133.2)\u00a0min, a decrease in k\u2010time by 0.6 (0.4\u20130.8)\u00a0min, an increase in alpha angle by 7.3 (5.5\u20139.0)\u00b0, and a decrease in maximum amplitude by 2.0 (0.6\u20133.4)\u00a0mm. This may have implications for epidural blood patch, as success may be reduced near the time of dural puncture when cerebrospinal fluid leak is at its greatest, and large volumes of blood may be required to reduce haemodilution and clot destabilisation by cerebrospinal fluid. In addition, blood patching should be performed at the level of the dural puncture in order to ensure that the maximum volume of blood comes into contact with the cerebrospinal fluid.<\/jats:p>","DOI":"10.1111\/anae.12911","type":"journal-article","created":{"date-parts":[[2014,11,27]],"date-time":"2014-11-27T01:35:03Z","timestamp":1417052103000},"page":"135-141","source":"Crossref","is-referenced-by-count":16,"title":["The effect of serial in vitro haemodilution with maternal cerebrospinal fluid and crystalloid on thromboelastographic (TEG\n                    <sup>\u00ae<\/sup>\n                    ) blood coagulation parameters, and the implications for epidural blood patching"],"prefix":"10.1111","volume":"70","author":[{"given":"S.","family":"Armstrong","sequence":"first","affiliation":[{"name":"Department of Anaesthesia Frimley Health NHS Foundation Trust Frimley UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Fernando","sequence":"additional","affiliation":[{"name":"Department of Anaesthesia University College London Hospitals NHS Trust London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Tamilselvan","sequence":"additional","affiliation":[{"name":"Department of Anaesthesia The Princess Alexandra Hospital Harlow UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Stewart","sequence":"additional","affiliation":[{"name":"Department of Anaesthesia University College London Hospitals NHS Trust London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Columb","sequence":"additional","affiliation":[{"name":"Department of Anaesthesia South Manchester University Hospitals NHS Trust Wythenshawe UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,11,27]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000542-200412000-00024"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.bja.a013451"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000539-199703000-00022"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/bja\/aei011"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2044.1980.tb03834.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1213\/ANE.0b013e318218204d"},{"key":"e_1_2_7_8_1","first-page":"309P","article-title":"Thromboelastograhy: validation of TEG 3000 and TEG 5000","volume":"86","author":"Robinson LS","year":"2001","journal-title":"British Journal of Anaesthesia"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000539-199707000-00017"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/bja\/82.1.110"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijoa.2007.04.009"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijoa.2004.06.002"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijoa.2013.04.012"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2044.2005.04222.x"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001503-199906000-00002"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0899-7071(99)00109-6"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1097\/00132586-198102000-00068"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1526-4610.1995.hed3507420.x"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000542-198809010-00409"},{"key":"e_1_2_7_20_1","first-page":"226","article-title":"Epidural injection of autologous blood for postlumbar\u2010puncture headache. II. Additional clinical experiences and laboratory investigation","volume":"51","author":"DiGiovanni AJ","year":"1972","journal-title":"Anesthesia and Analgesia"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/bja\/aeg231"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2044.2012.07101.x"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/anae.12748"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1213\/00000539-199005000-00017"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/bja\/aef179"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/bja\/aeq191"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/aas.12047"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/anae.12562"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2044.2002.02707.x"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000542-200108000-00012"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1093\/bja\/71.2.182"}],"container-title":["Anaesthesia"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fanae.12911","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/associationofanaesthetists-publications.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/anae.12911","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T01:09:42Z","timestamp":1761700182000},"score":1,"resource":{"primary":{"URL":"https:\/\/associationofanaesthetists-publications.onlinelibrary.wiley.com\/doi\/10.1111\/anae.12911"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,11,27]]},"references-count":30,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2015,2]]}},"alternative-id":["10.1111\/anae.12911"],"URL":"https:\/\/doi.org\/10.1111\/anae.12911","archive":["Portico"],"relation":{},"ISSN":["0003-2409","1365-2044"],"issn-type":[{"value":"0003-2409","type":"print"},{"value":"1365-2044","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,11,27]]}}}