{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T04:36:26Z","timestamp":1787891786924,"version":"build-2784847793"},"reference-count":34,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2015,12,29]],"date-time":"2015-12-29T00:00:00Z","timestamp":1451347200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003625","name":"Ministry of Health and Welfare","doi-asserted-by":"publisher","award":["A110076"],"award-info":[{"award-number":["A110076"]}],"id":[{"id":"10.13039\/501100003625","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Andrology"],"published-print":{"date-parts":[[2016,1]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The major hurdle for the clinical application of stem cell therapy is the heterogeneous nature of the isolated cells, which may cause different treatment outcomes. The aim of this study was to examine the effectiveness of mouse clonal bone marrow\u2010derived stem cells (<jats:styled-content style=\"fixed-case\">BMSC<\/jats:styled-content>s) obtained from a single colony by using subfractionation culturing method for erectile function in diabetic animals. Twelve\u2010week\u2010old C57<jats:styled-content style=\"fixed-case\">BL<\/jats:styled-content>\/6J mice were divided into four groups: controls, diabetic mice, and diabetic mice treated with a single intracavernous injection of <jats:styled-content style=\"fixed-case\">PBS<\/jats:styled-content> (20\u00a0\u03bcL) or clonal <jats:styled-content style=\"fixed-case\">BMSC<\/jats:styled-content>s (3\u00a0\u00d7\u00a010<jats:sup>5<\/jats:sup>\u00a0cells\/20\u00a0\u03bcL). Clonal <jats:styled-content style=\"fixed-case\">BMSC<\/jats:styled-content>s were isolated from 5\u2010week\u2010old C3H mice. Two weeks after treatment, erectile function was measured by electrical stimulation of the cavernous nerve. The penis was stained with antibodies to <jats:styled-content style=\"fixed-case\">PECAM<\/jats:styled-content>\u20101, smooth muscle \u03b1\u2010actin, neuronal nitric oxide synthase (<jats:styled-content style=\"fixed-case\">nNOS<\/jats:styled-content>), neurofilament, and phosphorylated endothelial <jats:styled-content style=\"fixed-case\">NOS<\/jats:styled-content> (phospho\u2010<jats:styled-content style=\"fixed-case\">eNOS<\/jats:styled-content>). We also performed Western blot for phospho\u2010<jats:styled-content style=\"fixed-case\">eNOS<\/jats:styled-content>, and <jats:styled-content style=\"fixed-case\">eNOS<\/jats:styled-content> in the corpus cavernosum tissue. Local delivery of clonal <jats:styled-content style=\"fixed-case\">BMSC<\/jats:styled-content>s significantly restored cavernous endothelial and smooth muscle cell contents, and penile <jats:styled-content style=\"fixed-case\">nNOS<\/jats:styled-content> and neurofilament contents, and induced <jats:styled-content style=\"fixed-case\">eNOS<\/jats:styled-content> phosphorylation (Ser1177) in diabetic mice. Intracavernous injection of clonal <jats:styled-content style=\"fixed-case\">BMSC<\/jats:styled-content>s induced significant recovery of erectile function, which reached 80\u201390% of the control values. Clonal <jats:styled-content style=\"fixed-case\">BMSC<\/jats:styled-content>s successfully restored erectile function through dual angiogenic and neurotrophic effects in diabetic mice. The homogenous nature of clonal mesenchymal stem cells may allow their clinical applications and open a new avenue through which to treat diabetic erectile dysfunction.<\/jats:p>","DOI":"10.1111\/andr.12138","type":"journal-article","created":{"date-parts":[[2015,12,29]],"date-time":"2015-12-29T08:02:46Z","timestamp":1451376166000},"page":"172-184","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Intracavernous delivery of clonal mesenchymal stem cells rescues erectile function in the streptozotocin\u2010induced diabetic mouse"],"prefix":"10.1111","volume":"4","author":[{"given":"J.\u2010K.","family":"Ryu","sequence":"first","affiliation":[{"name":"National Research Center for Sexual Medicine and Department of Urology Inha University School of Medicine Incheon Korea"},{"name":"Inha Research Institute for Medical Sciences Inha University School of Medicine Incheon Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.\u2010H.","family":"Kim","sequence":"additional","affiliation":[{"name":"Translational Research Center Inha University School of Medicine Incheon Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.\u2010M.","family":"Song","sequence":"additional","affiliation":[{"name":"National Research Center for Sexual Medicine and Department of Urology Inha University School of Medicine Incheon Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.\u2010S.","family":"Ryu","sequence":"additional","affiliation":[{"name":"Department of Urology Sungkyunkwan University School of Medicine Samsung Changwon Hospital Changwon Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.\u2010N.","family":"Kim","sequence":"additional","affiliation":[{"name":"Drug Development Program Department of Medicine Inha University School of Medicine Incheon Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.\u2010H.","family":"Shin","sequence":"additional","affiliation":[{"name":"SCM Lifescience Co., Ltd. Incheon Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Yi","sequence":"additional","affiliation":[{"name":"Translational Research Center Inha University School of Medicine Incheon Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.\u2010K.","family":"Suh","sequence":"additional","affiliation":[{"name":"National Research Center for Sexual Medicine and Department of Urology Inha University School of Medicine Incheon Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. 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