{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T22:14:42Z","timestamp":1762899282535},"reference-count":63,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2016,7,1]],"date-time":"2016-07-01T00:00:00Z","timestamp":1467331200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81571493"],"award-info":[{"award-number":["81571493"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Andrology"],"published-print":{"date-parts":[[2016,11]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Cell\u2010free seminal <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> (cfs\u2010<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>) is mixed transcripts derived from male reproductive organs, and is potential biomarker for the research and diagnosis of male reproductive\u2010related diseases. However, some clinical factors, including age, asymptomatic <jats:italic>Ureaplasma urealyticum<\/jats:italic> (<jats:styled-content style=\"fixed-case\">UU<\/jats:styled-content>) infection, scrotal heat stress, abstinence period, and the storage condition of semen samples, may interfere with sperm parameters and the measurement of seminal biomarkers. Accordingly, this study was designed to evaluate the effect of above clinical factors on the measurement of cfs\u2010<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>, aiming to lay a foundation for its research use and potential clinical application. Semen samples were collected according to the selected clinical factors. Cell\u2010free seminal plasma was obtained by centrifugation and total <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> was extracted with <jats:styled-content style=\"fixed-case\">TRI<\/jats:styled-content>zol <jats:styled-content style=\"fixed-case\">LS<\/jats:styled-content>. Selective male reproductive organ\u2010specific cfs\u2010<jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content>s and cfs\u2010mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s were quantified by quantitative real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>. The concentration and total amount of cfs\u2010<jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content>s and cfs\u2010mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s in one ejaculate were calculated and compared. <jats:italic><jats:styled-content style=\"fixed-case\">ACTB<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> DDX<\/jats:styled-content>4<\/jats:italic> (testis\u2010specific), <jats:italic><jats:styled-content style=\"fixed-case\">WFDC<\/jats:styled-content>9<\/jats:italic> (epididymis\u2010specific), and miR\u2010514a\u20103p (testis\u2010specific) significantly increased after scrotal heat stress. <jats:italic><jats:styled-content style=\"fixed-case\">SEMG<\/jats:styled-content>1<\/jats:italic> (seminal vesicle\u2010specific) showed declining tendency with the prolonged abstinence period. Age, asymptomatic <jats:styled-content style=\"fixed-case\">UU<\/jats:styled-content> infection, and the storage condition showed no significant impact on the measurement of cfs\u2010<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>. These results indicate that scrotal heat stress significantly interfere with the selected cfs\u2010<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> derived from the testis and epididymis, and abstinence period may affect the yield of cfs\u2010<jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> from seminal vesicle, while other clinical factors has no significant impact on the measurement. Thus, heat exposure and abstinence period should be considered for the cfs\u2010<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> measurement in its research or clinical application.<\/jats:p>","DOI":"10.1111\/andr.12238","type":"journal-article","created":{"date-parts":[[2016,7,2]],"date-time":"2016-07-02T00:53:24Z","timestamp":1467420804000},"page":"1010-1019","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Potential confounding factors in measurement of specific cell\u2010free seminal <scp>mRNA<\/scp>s and micro<scp>RNA<\/scp>s derived from human reproductive organs"],"prefix":"10.1111","volume":"4","author":[{"given":"T. T.","family":"Dong","sequence":"first","affiliation":[{"name":"Family Planning Research Institute\/Center of Reproductive Medicine Tongji Medical College Huazhong University of Science and Technology Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Q.","family":"Yu","sequence":"additional","affiliation":[{"name":"Family Planning Research Institute\/Center of Reproductive Medicine Tongji Medical College Huazhong University of Science and Technology Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"X. R.","family":"Qing","sequence":"additional","affiliation":[{"name":"Family Planning Research Institute\/Center of Reproductive Medicine Tongji Medical College Huazhong University of Science and Technology Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"X. L.","family":"Ma","sequence":"additional","affiliation":[{"name":"Family Planning Research Institute\/Center of Reproductive Medicine Tongji Medical College Huazhong University of Science and Technology Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W. W.","family":"Dong","sequence":"additional","affiliation":[{"name":"Family Planning Research Institute\/Center of Reproductive Medicine Tongji Medical College Huazhong University of Science and Technology Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Shi","sequence":"additional","affiliation":[{"name":"Family Planning Research Institute\/Center of Reproductive Medicine Tongji Medical College Huazhong University of Science and Technology Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. 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