{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T21:28:13Z","timestamp":1790630893724,"version":"4.1.0"},"reference-count":45,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2016,8,27]],"date-time":"2016-08-27T00:00:00Z","timestamp":1472256000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002642","name":"Korea University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002642","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Andrology"],"published-print":{"date-parts":[[2016,11]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Apolipoprotein D, a 29\u2010kDa secreted glycoprotein that belongs to the lipocalin superfamily, is widely expressed in various tissues and associated with lipid metabolism as a component of high\u2010density lipoproteins. Although Apolipoprotein D binds to small hydrophobic ligands including cholesterol, little is known about effects of high\u2010fat diet with cholesterol on expression of Apolipoprotein D in the male reproductive tract. Therefore, we investigated Apod expression in penises, prostate glands, and testes from rats fed a high\u2010fat diet including a high amount of cholesterol. Our previous research indicated that a high\u2010fat diet induces dyslipidemia leading to histological changes and dysfunction of male reproduction in rats. Consistent with these results, <jats:italic>Apod<\/jats:italic> m<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> expression was significantly (<jats:italic>p\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>0.001) decreased in penises and prostate glands (<jats:italic>p\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>0.01) and testes (<jats:italic>p\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>0.01) from rats fed a high\u2010fat diet as compared with normal diet. In addition, <jats:italic>Apod<\/jats:italic> m<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> and protein were detected predominantly in urethral epithelium and penile follicle from rats. Moreover, changes in expression of specific micro<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s (<jats:italic>miR\u2010229b\u20103p<\/jats:italic>,<jats:italic> miR\u2010423\u20103p<\/jats:italic>, and <jats:italic>miR\u2010490\u20103p<\/jats:italic>) regulating <jats:italic>Apod<\/jats:italic> in the penises and prostate glands were negatively associated with <jats:italic>Apod<\/jats:italic> expression. Collectively, results of this study suggest that <jats:italic>Apod<\/jats:italic> is a novel regulatory gene in the male reproductive system, especially in penises of rats fed a high\u2010cholesterol diet, and that expression of <jats:italic>Apod<\/jats:italic> is regulated at the posttranscriptional level by target microRNAs.<\/jats:p>","DOI":"10.1111\/andr.12250","type":"journal-article","created":{"date-parts":[[2016,8,27]],"date-time":"2016-08-27T06:17:06Z","timestamp":1472278626000},"page":"1115-1122","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Differential expression of apolipoprotein D in male reproductive system of rats by high\u2010fat diet"],"prefix":"10.1111","volume":"4","author":[{"given":"W.","family":"Lim","sequence":"first","affiliation":[{"name":"Institute of Animal Molecular Biotechnology and Department of Biotechnology College of Life Sciences and Biotechnology Korea University Seoul Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Bae","sequence":"additional","affiliation":[{"name":"Institute of Animal Molecular Biotechnology and Department of Biotechnology College of Life Sciences and Biotechnology Korea University Seoul Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Song","sequence":"additional","affiliation":[{"name":"Institute of Animal Molecular Biotechnology and Department of Biotechnology College of Life Sciences and Biotechnology Korea University Seoul Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,8,27]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00109-009-0462-3"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(01)67123-4"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/jsm.12793"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(04)00045-5"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/107.7.1340"},{"key":"e_1_2_9_7_1","doi-asserted-by":"crossref","first-page":"1726","DOI":"10.1093\/jn\/110.8.1726","article-title":"Second report of the American Institute of Nutrition ad hoc committee on standards for nutritional studies","volume":"110","author":"Bieri IG","year":"1980","journal-title":"J. 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