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The aim of this study was to evaluate in frozen\/thawed bovine spermatozoa the effect of several compounds, such as myo\u2010inositol, pentoxifylline, penicillamine + hypotaurine + epinephrine mixture (\n                    <jats:styled-content style=\"fixed-case\">PHE<\/jats:styled-content>\n                    ), caffeine and coenzyme Q10+ zinc +\n                    <jats:sc>d<\/jats:sc>\n                    \u2010aspartate mixture (\n                    <jats:styled-content style=\"fixed-case\">CZA<\/jats:styled-content>\n                    ), on either kinetic or metabolic parameters. Sperm kinetics was evaluated by Sperm Class Analyser whereas specific fluorochromes were used to evaluated mitochondrial membrane potential (\n                    <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\n                    ), intracellular\n                    <jats:styled-content style=\"fixed-case\">pH<\/jats:styled-content>\n                    , intracellular calcium concentration and lipid peroxidation. Lipid peroxidation was also evaluated by\n                    <jats:styled-content style=\"fixed-case\">TBARS<\/jats:styled-content>\n                    analysis. Treatments significantly affected total and progressive motility with different dynamics in relation to the incubation time. After the first hour of incubation,\n                    <jats:styled-content style=\"fixed-case\">CZA<\/jats:styled-content>\n                    treatment produced the best performance in total and progressive sperm motility as well as in curvilinear velocity, average path velocity and amplitude of head displacement, whereas pentoxifylline stimulated the highest straight\u2010line velocity.\n                    <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\n                    showed higher values (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a00.01) after treatment with pentoxifylline and\n                    <jats:styled-content style=\"fixed-case\">PHE<\/jats:styled-content>\n                    . Intracytoplasmic calcium concentration and lipid peroxidation were significantly (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a00.05) affected by the incubation time rather than the treatments. Intracellular\n                    <jats:styled-content style=\"fixed-case\">pH<\/jats:styled-content>\n                    varied significantly (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a00.01) in relation to either the incubation time or treatments. In particular, it showed a progressive increase throughout incubation with values in control group significantly higher than in myo\u2010inositol,\n                    <jats:styled-content style=\"fixed-case\">PHE<\/jats:styled-content>\n                    , caffeine, pentoxifylline and\n                    <jats:styled-content style=\"fixed-case\">CZA<\/jats:styled-content>\n                    groups (7.37\u00a0\u00b1\u00a00.03 vs. 7.29\u00a0\u00b1\u00a00.03, 7.28\u00a0\u00b1\u00a00.03, 7.26\u00a0\u00b1\u00a00.03, 7.22\u00a0\u00b1\u00a00.03 and 7.00\u00a0\u00b1\u00a00.03, respectively;\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a00.01).; however, among treatments,\n                    <jats:styled-content style=\"fixed-case\">CZA<\/jats:styled-content>\n                    displayed the lowest values. Significant correlations were found between sperm kinetic and metabolic parameters. These findings provide new comparative information on the effects of putative metabolic enhancers on kinetics and metabolic activities of bovine spermatozoa. In this study, a rapid methodological approach for evaluating sperm quality is proposed.\n                  <\/jats:p>","DOI":"10.1111\/andr.12259","type":"journal-article","created":{"date-parts":[[2016,10,21]],"date-time":"2016-10-21T17:42:58Z","timestamp":1477071778000},"page":"133-145","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Kinetic activity, membrane mitochondrial potential, lipid peroxidation, intracellular\n                    <scp>pH<\/scp>\n                    and calcium of frozen\/thawed bovine spermatozoa treated with metabolic enhancers"],"prefix":"10.1111","volume":"5","author":[{"given":"R.","family":"Boni","sequence":"first","affiliation":[{"name":"Department of Sciences University of Basilicata Potenza Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Gallo","sequence":"additional","affiliation":[{"name":"Department of Biology and Evolution of Marine 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