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Extracellular <jats:styled-content style=\"fixed-case\">cAMP<\/jats:styled-content> can be sequentially degraded into 5\u2032<jats:styled-content style=\"fixed-case\">AMP<\/jats:styled-content> and adenosine by ecto\u2010phosphodiesterases (ecto\u2010<jats:styled-content style=\"fixed-case\">PDE<\/jats:styled-content>) and ecto\u2010nucleotidases, a phenomenon called extracellular <jats:styled-content style=\"fixed-case\">cAMP<\/jats:styled-content>\u2010adenosine pathway. As <jats:styled-content style=\"fixed-case\">cAMP<\/jats:styled-content>\u2010adenosine pathway is involved in sperm capacitation, we hypothesize that extracellular <jats:styled-content style=\"fixed-case\">PDE<\/jats:styled-content>s are functionally present in seminal plasma. Exclusively measuring <jats:styled-content style=\"fixed-case\">cAMP<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PDE<\/jats:styled-content> activity, total activity in bovine seminal plasma was 10.1\u00a0\u00b1\u00a01.5 fmoles\/min\/\u03bcg. Using different family\u2010specific <jats:styled-content style=\"fixed-case\">PDE<\/jats:styled-content> inhibitors, we showed that in seminal plasma, the major <jats:styled-content style=\"fixed-case\">cAMP<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PDE<\/jats:styled-content> activity was papaverine sensitive (47.5%). These data support the presence of <jats:styled-content style=\"fixed-case\">PDE<\/jats:styled-content>10 in bovine seminal plasma and was further confirmed by western blot. In epididymal fluid, total <jats:styled-content style=\"fixed-case\">cAMP<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PDE<\/jats:styled-content> activity was 48.2\u00a0\u00b1\u00a014.8 fmoles\/min\/\u03bcg and we showed that the major <jats:styled-content style=\"fixed-case\">cAMP<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PDE<\/jats:styled-content> activity was 3\u2010isobutyl\u2010methylxanthine insensitive and thus ascribed to <jats:styled-content style=\"fixed-case\">PDE<\/jats:styled-content>8 family. <jats:styled-content style=\"fixed-case\">PDE<\/jats:styled-content>10A <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content>s were found in the testis, epididymis, and seminal vesicles. <jats:styled-content style=\"fixed-case\">cAMP<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PDE<\/jats:styled-content> activity is present in bovine seminal plasma and epididymal fluid. The results suggest a role for ecto\u2010<jats:styled-content style=\"fixed-case\">PDE<\/jats:styled-content>s present in those fluids in the signaling pathways involved in sperm functions.<\/jats:p>","DOI":"10.1111\/andr.12267","type":"journal-article","created":{"date-parts":[[2016,8,27]],"date-time":"2016-08-27T06:17:00Z","timestamp":1472278620000},"page":"1123-1130","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Characterization of <scp>cAMP<\/scp>\u2010phosphodiesterase activity in bovine seminal plasma"],"prefix":"10.1111","volume":"4","author":[{"given":"A.","family":"Bergeron","sequence":"first","affiliation":[{"name":"Centre de Recherche en Reproduction D\u00e9veloppement et Sant\u00e9 Interg\u00e9n\u00e9rationnelle Universit\u00e9 Laval Qu\u00e9bec QC Canada"},{"name":"D\u00e9partement des Sciences Animales Facult\u00e9 des Sciences de L'agriculture et de L'alimentation Universit\u00e9 Laval Qu\u00e9bec QC Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. 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