{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T00:32:21Z","timestamp":1778113941160,"version":"3.51.4"},"reference-count":47,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2016,11,16]],"date-time":"2016-11-16T00:00:00Z","timestamp":1479254400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Andrology"],"published-print":{"date-parts":[[2017,1]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Cyclic adenosine monophosphate (<jats:styled-content style=\"fixed-case\">cAMP<\/jats:styled-content>) plays a crucial role as a signaling molecule for capacitation, motility, and acrosome reaction in mammalian spermatozoa. It is well\u2010known that <jats:styled-content style=\"fixed-case\">cAMP<\/jats:styled-content> degradation by phosphodiesterase (<jats:styled-content style=\"fixed-case\">PDE<\/jats:styled-content>) enzyme has a major impact on sperm functions. This study was undertaken to characterize <jats:styled-content style=\"fixed-case\">cAMP<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PDE<\/jats:styled-content> activity in bovine spermatozoa. Total <jats:styled-content style=\"fixed-case\">cAMP<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PDE<\/jats:styled-content> activity in cauda epididymal and ejaculated spermatozoa was 543.2\u00a0\u00b1\u00a049.5 and 1252.6\u00a0\u00b1\u00a086.5 fmoles\/min\/10<jats:sup>6<\/jats:sup> spermatozoa, respectively. Using different family\u2010specific <jats:styled-content style=\"fixed-case\">PDE<\/jats:styled-content> inhibitors, we showed that in cauda epididymal and ejaculated spermatozoa, the major <jats:styled-content style=\"fixed-case\">cAMP<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PDE<\/jats:styled-content> activity was papaverine\u2010sensitive (44.5% and 57.5%, respectively, at 400\u00a0n<jats:sc>m<\/jats:sc>, papaverine is a specific inhibitor of the <jats:styled-content style=\"fixed-case\">PDE<\/jats:styled-content>10 family). These data are supporting the functional presence of <jats:styled-content style=\"fixed-case\">PDE<\/jats:styled-content>10 in bovine spermatozoa and were further confirmed by western blot to be <jats:styled-content style=\"fixed-case\">PDE<\/jats:styled-content>10A. Using immunocytochemistry, we showed immunoreactive signal for <jats:styled-content style=\"fixed-case\">PDE<\/jats:styled-content>10A present on the post\u2010acrosomal region of the head and on the flagella of ejaculated spermatozoa. Using papaverine, we showed that it promotes tyrosine phosphorylation of sperm proteins, phosphorylation of Erk1 and Erk2, and Ca<jats:sup>2+<\/jats:sup> release from Ca<jats:sup>2+<\/jats:sup> store. These results suggest that <jats:styled-content style=\"fixed-case\">PDE<\/jats:styled-content>10 is functionally present in bovine spermatozoa and is affecting different molecular events involved in capacitation, most probably by <jats:styled-content style=\"fixed-case\">cAMP<\/jats:styled-content> local regulation.<\/jats:p>","DOI":"10.1111\/andr.12290","type":"journal-article","created":{"date-parts":[[2016,11,17]],"date-time":"2016-11-17T03:51:52Z","timestamp":1479354712000},"page":"169-179","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Papaverine\u2010sensitive phosphodiesterase activity is measured in bovine spermatozoa"],"prefix":"10.1111","volume":"5","author":[{"given":"A.","family":"Bergeron","sequence":"first","affiliation":[{"name":"Centre de Recherche en Reproduction D\u00e9veloppement et Sant\u00e9 Interg\u00e9n\u00e9rationnelle Universit\u00e9 Laval Qu\u00e9bec QC Canada"},{"name":"D\u00e9partement des Sciences Animales Facult\u00e9 des Sciences de L'agriculture et de L'alimentation Universit\u00e9 Laval Qu\u00e9bec QC Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"H\u00e9bert","sequence":"additional","affiliation":[{"name":"Centre de Recherche en Reproduction D\u00e9veloppement et Sant\u00e9 Interg\u00e9n\u00e9rationnelle Universit\u00e9 Laval Qu\u00e9bec QC Canada"},{"name":"D\u00e9partement des Sciences Animales Facult\u00e9 des Sciences de L'agriculture et de L'alimentation Universit\u00e9 Laval Qu\u00e9bec QC Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Guillemette","sequence":"additional","affiliation":[{"name":"Centre de Recherche en Reproduction D\u00e9veloppement et Sant\u00e9 Interg\u00e9n\u00e9rationnelle Universit\u00e9 Laval Qu\u00e9bec QC Canada"},{"name":"D\u00e9partement des Sciences Animales Facult\u00e9 des Sciences de L'agriculture et de L'alimentation Universit\u00e9 Laval Qu\u00e9bec QC Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Laroche","sequence":"additional","affiliation":[{"name":"Centre de Recherche en Reproduction D\u00e9veloppement et Sant\u00e9 Interg\u00e9n\u00e9rationnelle Universit\u00e9 Laval Qu\u00e9bec QC Canada"},{"name":"D\u00e9partement des Sciences Animales Facult\u00e9 des Sciences de L'agriculture et de L'alimentation Universit\u00e9 Laval Qu\u00e9bec QC Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.\u2010P.","family":"Poulin","sequence":"additional","affiliation":[{"name":"Centre de Recherche en Reproduction D\u00e9veloppement et Sant\u00e9 Interg\u00e9n\u00e9rationnelle Universit\u00e9 Laval Qu\u00e9bec QC Canada"},{"name":"D\u00e9partement des Sciences Animales Facult\u00e9 des Sciences de L'agriculture et de L'alimentation Universit\u00e9 Laval Qu\u00e9bec QC Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. 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