{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,19]],"date-time":"2024-07-19T06:32:18Z","timestamp":1721370738369},"reference-count":35,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2016,12,19]],"date-time":"2016-12-19T00:00:00Z","timestamp":1482105600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100005703","name":"Akdeniz \u00dcniversitesi","doi-asserted-by":"publisher","award":["2010.04.0103.006"],"award-info":[{"award-number":["2010.04.0103.006"]}],"id":[{"id":"10.13039\/501100005703","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Andrology"],"published-print":{"date-parts":[[2017,3]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Varicocele is ordinarily accompanied by testicular damage and male infertility. Several theories have been proposed to explain the detrimental effect of varicocele on testis tissue, including the possible effects of oxidative stress. The poly(<jats:styled-content style=\"fixed-case\">ADP<\/jats:styled-content>\u2010ribose) polymerase (<jats:styled-content style=\"fixed-case\">PARP<\/jats:styled-content>) pathway has been established as a major downstream intracellular pathway of oxidative stress. Recently we have reported that <jats:styled-content style=\"fixed-case\">PARP<\/jats:styled-content> pathway has been activated in varicocele\u2010induced rat testicular damage model. The aim of the present study was to investigate the possible protective effect of <jats:styled-content style=\"fixed-case\">PARP<\/jats:styled-content> inhibition in varicocele\u2010associated testicular damage. Fifty male Wistar rats were divided into five groups: control, sham, varicocele\u2010induced, varicocele\u2010induced 1,5\u2010isoquinolinediol (<jats:styled-content style=\"fixed-case\">ISO<\/jats:styled-content>, a <jats:styled-content style=\"fixed-case\">PARP<\/jats:styled-content> inhibitor)\u2010treated, and <jats:styled-content style=\"fixed-case\">ISO<\/jats:styled-content> treated groups. The <jats:styled-content style=\"fixed-case\">ISO<\/jats:styled-content>\u2010treated rats received intraperitoneal injections of 3\u00a0mg\/kg <jats:styled-content style=\"fixed-case\">ISO<\/jats:styled-content> daily for 13\u00a0weeks. After 13\u00a0weeks of varicocele induction, body and testes weights were investigated in all groups. Histopathology of testes were evaluated by light microscopy. Expressions of <jats:styled-content style=\"fixed-case\">PAR<\/jats:styled-content>, p53 and cytochrome <jats:italic>c<\/jats:italic> were detected by immunohistochemistry and cleaved <jats:styled-content style=\"fixed-case\">PARP<\/jats:styled-content>\u20101, <jats:styled-content style=\"fixed-case\">PAR<\/jats:styled-content>, p53 and cytochrome <jats:italic>c<\/jats:italic> by western blot. The degree of apoptosis was determined by <jats:styled-content style=\"fixed-case\">TUNEL<\/jats:styled-content>. Light microscopy revealed testicular damage comprising various degrees of seminiferous tubule degeneration in varicocele\u2010induced rats and their testes weights decreased significantly, whereas <jats:styled-content style=\"fixed-case\">ISO<\/jats:styled-content> administration prevented it. Expressions of cleaved <jats:styled-content style=\"fixed-case\">PARP<\/jats:styled-content>\u20101, <jats:styled-content style=\"fixed-case\">PAR<\/jats:styled-content>, cytochrome <jats:italic>c<\/jats:italic>, and p53 increased significantly in varicocele\u2010induced rats, whereas the level of these molecules were similar to controls in varicocele\u2010induced rats treated with <jats:styled-content style=\"fixed-case\">ISO<\/jats:styled-content>. In conclusion, increased <jats:styled-content style=\"fixed-case\">PARP<\/jats:styled-content> activation in testes seems to be related with testicular damage and apoptosis associated with varicocele and pharmacological inhibition of this pathway might be an effective intervention to prevent varicocele\u2010induced testicular injury.<\/jats:p>","DOI":"10.1111\/andr.12305","type":"journal-article","created":{"date-parts":[[2016,12,19]],"date-time":"2016-12-19T23:03:45Z","timestamp":1482188625000},"page":"362-369","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Inhibition of poly(<scp>ADP<\/scp>\u2010ribose) polymerase may have preventive potential for varicocoele\u2010associated testicular damage in rats"],"prefix":"10.1111","volume":"5","author":[{"given":"C.","family":"Celik\u2010Ozenci","sequence":"first","affiliation":[{"name":"Department of Histology and Embryology Akdeniz University School of Medicine Antalya Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"Kuscu","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology Akdeniz University School of Medicine Antalya Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N. 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