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In the recent years, functional characterization of these proteins is being studied by genetic manipulations that involve targeted over expression or knock down of specific genes. In this study, we adopted <jats:styled-content style=\"fixed-case\">FuGENE<\/jats:styled-content> 6\u2010based in\u00a0vivo transfection of rat cauda epididymis with p<jats:styled-content style=\"fixed-case\">GFP<\/jats:styled-content>\u2010V\u2010<jats:styled-content style=\"fixed-case\">RS<\/jats:styled-content> plasmid that encodes sh<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> to knock down <jats:italic>Pate<\/jats:italic> m<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> levels to implicate a possible role for this gene in sperm function. The m<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> levels of <jats:italic>Pate<\/jats:italic> gene were significantly decreased in <jats:styled-content style=\"fixed-case\">HEK<\/jats:styled-content> cells as well as in cauda of rats upon transfection with p<jats:styled-content style=\"fixed-case\">GFP<\/jats:styled-content>\u2010V\u2010<jats:styled-content style=\"fixed-case\">RS<\/jats:styled-content> plasmid that encodes sh<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> targeting <jats:italic>Pate<\/jats:italic> gene. Spermatozoa obtained from the transfected cauda displayed impairment in capacitation and acrosome reaction. Furthermore, rats subjected to in\u00a0vivo transfection to knock down <jats:italic>Pate<\/jats:italic> m<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> levels had compromised fertility. Results of our study indicate a role for <jats:italic>Pate<\/jats:italic> gene in sperm function and can be exploited as a potential male contraceptive target.<\/jats:p>","DOI":"10.1111\/andr.12321","type":"journal-article","created":{"date-parts":[[2017,2,20]],"date-time":"2017-02-20T04:42:41Z","timestamp":1487565761000},"page":"541-547","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["sh<scp>RNA<\/scp> mediated ablation of prostate and testis expressed (<i>Pate<\/i>) messenger <scp>RNA<\/scp> results in impaired sperm function and fertility"],"prefix":"10.1111","volume":"5","author":[{"given":"A.","family":"Rajesh","sequence":"first","affiliation":[{"name":"Department of Animal Biology University of Hyderabad Gachibowli Hyderabad India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Yenugu","sequence":"additional","affiliation":[{"name":"Department of Animal Biology University of 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