{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T17:48:46Z","timestamp":1792086526951,"version":"4.4.0"},"reference-count":67,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2017,7,17]],"date-time":"2017-07-17T00:00:00Z","timestamp":1500249600000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Italian Ministry of Education, University and Research","award":["2015XCR88M"],"award-info":[{"award-number":["2015XCR88M"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Andrology"],"published-print":{"date-parts":[[2017,7]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Considering the widespread use of assisted reproductive techniques (\n                    <jats:styled-content style=\"fixed-case\">ART<\/jats:styled-content>\n                    ),\n                    <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\n                    methylation of specific genes involved in spermatogenesis achieves increasingly clinical relevance, representing a possible explanation of increased incidence of syndromes related to genomic imprinting in medically assisted pregnancies. Several trials suggested a relationship between male sub\u2010fertility and sperm\n                    <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\n                    methylation, although its weight on seminal parameters alteration is still a matter of debate. To evaluate whether aberrant sperm\n                    <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\n                    methylation of imprinted genes is associated with impaired sperm parameters. Meta\u2010analysis of controlled clinical trials evaluating imprinted genes sperm\n                    <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\n                    methylation comparing men with idiopathic infertility to fertile controls. Twenty\u2010four studies were included, allowing a meta\u2010analytic evaluation for\n                    <jats:italic>H19<\/jats:italic>\n                    ,\n                    <jats:italic>\n                      <jats:styled-content style=\"fixed-case\">MEST<\/jats:styled-content>\n                    <\/jats:italic>\n                    ,\n                    <jats:italic>\n                      <jats:styled-content style=\"fixed-case\">SNRPN<\/jats:styled-content>\n                    <\/jats:italic>\n                    , and\n                    <jats:italic>\n                      <jats:styled-content style=\"fixed-case\">LINE<\/jats:styled-content>\n                      \u20101\n                    <\/jats:italic>\n                    . When a high heterogeneity of the results was demonstrated, the random effect model was used.\n                    <jats:italic>H19<\/jats:italic>\n                    methylation levels resulted significantly lower in 879 infertile compared with 562 fertile men (7.53%, 95%\n                    <jats:styled-content style=\"fixed-case\">CI<\/jats:styled-content>\n                    : 5.14\u20139.93%,\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a00.001), suggesting a 9.91\u2010fold higher risk ratio to show aberrant sperm\n                    <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\n                    methylation (95%\n                    <jats:styled-content style=\"fixed-case\">CI<\/jats:styled-content>\n                    : 5.55\u201317.70,\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a00.001, I\n                    <jats:sup>2\u00a0<\/jats:sup>\n                    =\u00a019%) in infertile men. The mean\n                    <jats:italic>\n                      <jats:styled-content style=\"fixed-case\">MEST<\/jats:styled-content>\n                    <\/jats:italic>\n                    methylation level was significantly higher in 846 infertile compared with 353 fertile men (3.35%, 95%\n                    <jats:styled-content style=\"fixed-case\">CI<\/jats:styled-content>\n                    : 1.41\u20135.29%,\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a00.001), as well as for\n                    <jats:italic>\n                      <jats:styled-content style=\"fixed-case\">SNRPN<\/jats:styled-content>\n                    <\/jats:italic>\n                    comparing 301 infertile men with 124 controls (3.23%, 95%\n                    <jats:styled-content style=\"fixed-case\">CI<\/jats:styled-content>\n                    : 0.75\u20135.72%,\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a00.001).\n                    <jats:italic>\n                      <jats:styled-content style=\"fixed-case\">LINE<\/jats:styled-content>\n                      \u20101\n                    <\/jats:italic>\n                    methylation levels did not differ between 291 infertile men and 198 controls (0.44%, 95%\n                    <jats:styled-content style=\"fixed-case\">CI<\/jats:styled-content>\n                    : \u22122.04\u20131.16%,\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0=\u00a00.63). The meta\u2010analytic approach demonstrated that male infertility is associated with altered sperm methylation at\n                    <jats:italic>\n                      H19,\n                      <jats:styled-content style=\"fixed-case\">MEST<\/jats:styled-content>\n                    <\/jats:italic>\n                    , and\n                    <jats:italic>\n                      <jats:styled-content style=\"fixed-case\">SNRPN<\/jats:styled-content>\n                    <\/jats:italic>\n                    . Although its role in infertility remains unclear, sperm\n                    <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\n                    methylation could be associated with the epigenetic risk in\n                    <jats:styled-content style=\"fixed-case\">ART<\/jats:styled-content>\n                    . In this setting, before proposing this analysis in clinical practice, an accurate identification of the most representative genes and a cost\u2010effectiveness evaluation should be assessed in ad hoc prospective studies.\n                  <\/jats:p>","DOI":"10.1111\/andr.12379","type":"journal-article","created":{"date-parts":[[2017,7,18]],"date-time":"2017-07-18T08:25:49Z","timestamp":1500366349000},"page":"695-703","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":163,"title":["Impairment of sperm\n                    <scp>DNA<\/scp>\n                    methylation in male infertility: a meta\u2010analytic study"],"prefix":"10.1111","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6607-7105","authenticated-orcid":false,"given":"D.","family":"Santi","sequence":"first","affiliation":[{"name":"Unit of Endocrinology Department of Biomedical, Metabolic and Neural Sciences University of Modena and Reggio Emilia Modena Italy"},{"name":"Unit of Endocrinology Department of Medicine, Endocrinology, Metabolism and Geriatrics Azienda OU of Modena Modena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"De Vincentis","sequence":"additional","affiliation":[{"name":"Unit of Endocrinology Department of Biomedical, Metabolic and Neural Sciences University of Modena and Reggio Emilia Modena Italy"},{"name":"Unit of Endocrinology Department of Medicine, Endocrinology, Metabolism and Geriatrics Azienda OU of Modena Modena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Magnani","sequence":"additional","affiliation":[{"name":"Unit of Endocrinology Department of Biomedical, Metabolic and Neural Sciences University of Modena and Reggio Emilia Modena Italy"},{"name":"Unit of Endocrinology Department of Medicine, Endocrinology, Metabolism and Geriatrics Azienda OU of Modena Modena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Spaggiari","sequence":"additional","affiliation":[{"name":"Unit of Endocrinology Department of Biomedical, Metabolic and Neural Sciences University of Modena and Reggio Emilia Modena Italy"},{"name":"Unit of Endocrinology Department of Medicine, Endocrinology, Metabolism and Geriatrics Azienda OU of Modena Modena 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