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Testosterone administration improves physical function in some studies; however, its effects on serum circulating <jats:styled-content style=\"fixed-case\">CAF<\/jats:styled-content> concentrations remain unknown. Here we evaluate the effects of testosterone administration on circulating <jats:styled-content style=\"fixed-case\">CAF<\/jats:styled-content> levels in mobility\u2010limited men with low testosterone aged 65 or older participating in the Testosterone in Older Men with Mobility Limitations (<jats:styled-content style=\"fixed-case\">TOM<\/jats:styled-content>) Trial. We analyzed the difference in change in serum <jats:styled-content style=\"fixed-case\">CAF<\/jats:styled-content> levels between testosterone and placebo groups, as well as its association with muscle strength and physical function. Association of change in serum <jats:styled-content style=\"fixed-case\">CAF<\/jats:styled-content> levels with serum total (<jats:styled-content style=\"fixed-case\">TT<\/jats:styled-content>) and free testosterone (<jats:styled-content style=\"fixed-case\">FT<\/jats:styled-content>) was also evaluated. Men randomized to testosterone experienced significant improvement in muscle strength and physical function (assessed by loaded stair\u2010climbing power). However; testosterone administration was not associated with a reduction in serum <jats:styled-content style=\"fixed-case\">CAF<\/jats:styled-content> levels (effect size\u00a0=\u00a0\u221250.3\u00a0p<jats:sc>m<\/jats:sc>; 95% <jats:styled-content style=\"fixed-case\">CI<\/jats:styled-content>\u00a0=\u00a0\u2212162.1 to 61.5\u00a0p<jats:sc>m<\/jats:sc>;<jats:italic> p<\/jats:italic>\u00a0=\u00a00.374); there was no association between changes in <jats:styled-content style=\"fixed-case\">CAF<\/jats:styled-content> levels with changes in <jats:styled-content style=\"fixed-case\">TT<\/jats:styled-content> (<jats:italic>p<\/jats:italic>\u00a0=\u00a00.670) or <jats:styled-content style=\"fixed-case\">FT<\/jats:styled-content> (<jats:italic>p<\/jats:italic>\u00a0=\u00a00.747). There was no association between changes in serum <jats:styled-content style=\"fixed-case\">CAF<\/jats:styled-content> levels with improvement in either muscle strength or stair\u2010climbing power. In conclusion, testosterone treatment in mobility\u2010limited older men with low to low\u2010normal testosterone levels did not reduce serum <jats:styled-content style=\"fixed-case\">CAF<\/jats:styled-content> levels. Additionally, testosterone\u2010induced improvements in muscle strength and physical function were not associated with changes in serum <jats:styled-content style=\"fixed-case\">CAF<\/jats:styled-content> concentrations. These findings suggest that improvement in physical function with testosterone replacement in older men with mobility limitations and elevated <jats:styled-content style=\"fixed-case\">CAF<\/jats:styled-content> levels is mediated by mechanisms other than stabilization of the NMJ.<\/jats:p>","DOI":"10.1111\/andr.12424","type":"journal-article","created":{"date-parts":[[2017,9,26]],"date-time":"2017-09-26T21:12:46Z","timestamp":1506460366000},"page":"29-36","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Testosterone does not affect agrin cleavage in mobility\u2010limited older men despite improvement in physical function"],"prefix":"10.1111","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2960-1059","authenticated-orcid":false,"given":"T.","family":"Gagliano\u2010Juc\u00e1","sequence":"first","affiliation":[{"name":"Research Program in Men's Health: Aging and Metabolism Brigham and Women's Hospital Harvard Medical School Boston MA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T. W.","family":"Storer","sequence":"additional","affiliation":[{"name":"Research Program in Men's Health: Aging and Metabolism Brigham and Women's Hospital Harvard Medical School Boston MA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K. M.","family":"Pencina","sequence":"additional","affiliation":[{"name":"Research Program in Men's Health: Aging and Metabolism Brigham and Women's Hospital Harvard Medical School Boston MA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T. G.","family":"Travison","sequence":"additional","affiliation":[{"name":"Program on Aging Hebrew SeniorLife Roslindale MA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Z.","family":"Li","sequence":"additional","affiliation":[{"name":"Research Program in Men's Health: Aging and Metabolism Brigham and Women's Hospital Harvard Medical School Boston MA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Huang","sequence":"additional","affiliation":[{"name":"Research Program in Men's Health: Aging and Metabolism Brigham and Women's Hospital Harvard Medical School Boston MA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Hettwer","sequence":"additional","affiliation":[{"name":"Neurotune AG Schlieren Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Dahinden","sequence":"additional","affiliation":[{"name":"Neurotune AG Schlieren 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