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Sperm genomic quality is variable following cancer diagnosis, so its assessment is important if sperm cryopreservation is being considered. Here, we evaluated <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> damage using two <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> damage assays: an alkaline and for the first time, a neutral Comet assays in men presenting with testicular cancer (<jats:italic>n<\/jats:italic>\u00a0=\u00a019 for alkaline and 13 for neutral group) and lymphoma (<jats:italic>n<\/jats:italic>\u00a0=\u00a013 for alkaline and 09 for neutral group) compared with fertile donors (<jats:italic>n<\/jats:italic>\u00a0=\u00a020 for alkaline and 14 for neutral group). No significant differences were observed in any semen analysis parameters. In contrast, sperm <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> damage was higher in men with testicular cancer than in donors as assessed by both the alkaline (12.4% vs. 37.4%, <jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.001) and neutral (7.5% vs. 13.4%; <jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05) Comet assays. Similar trends were observed in men with lymphoma. Here, sperm <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> damage was higher using both the alkaline (35.0% vs. 12.4%) and neutral (10.7% against 7.5% (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05) Comet assays. Moreover, the <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> strand breaks (particularly double\u2010strand breaks) were significantly more prominent in men with cancer having abnormal seminal parameters than normozoospermic ones. This study showed that sperm <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> testing using alkaline and neutral Comet assays is more sensitive than semen analysis in detecting impaired sperm quality in men presenting with cancer. It may provide a useful adjunct when considering storage prior to cancer investigations and assisted reproductive techniques (<jats:styled-content style=\"fixed-case\">ART<\/jats:styled-content>)\u2010based treatment.<\/jats:p>","DOI":"10.1111\/andr.12429","type":"journal-article","created":{"date-parts":[[2017,9,26]],"date-time":"2017-09-26T21:06:50Z","timestamp":1506460010000},"page":"230-235","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Evaluation of sperm <scp>DNA<\/scp> quality in men presenting with testicular cancer and lymphoma using alkaline and neutral Comet assays"],"prefix":"10.1111","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7031-4027","authenticated-orcid":false,"given":"K.","family":"Kumar","sequence":"first","affiliation":[{"name":"Centre for Public Health Queen's University Belfast Belfast Northern Ireland UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Lewis","sequence":"additional","affiliation":[{"name":"Centre for Public Health Queen's University Belfast Belfast Northern Ireland UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Vinci","sequence":"additional","affiliation":[{"name":"Sexual Medicine and Andrology Unit Department of Clinical and Experimental Biomedical Sciences \u201cMario Serio\u201d University of Florence Florence Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Riera\u2010Escamilla","sequence":"additional","affiliation":[{"name":"Sexual Medicine and Andrology Unit Department of Clinical and Experimental Biomedical Sciences \u201cMario Serio\u201d University of Florence Florence Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.\u2010G.","family":"Fino","sequence":"additional","affiliation":[{"name":"Sexual Medicine and Andrology Unit Department of Clinical and Experimental Biomedical Sciences \u201cMario Serio\u201d University of Florence Florence Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Tamburrino","sequence":"additional","affiliation":[{"name":"Sexual Medicine and Andrology Unit Department of Clinical and Experimental Biomedical Sciences \u201cMario Serio\u201d University of Florence Florence Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Muratori","sequence":"additional","affiliation":[{"name":"Sexual Medicine and Andrology Unit Department of Clinical and Experimental Biomedical Sciences \u201cMario Serio\u201d University of Florence Florence Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Larsen","sequence":"additional","affiliation":[{"name":"Cryos International Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Krausz","sequence":"additional","affiliation":[{"name":"Sexual Medicine and Andrology Unit Department of Clinical and Experimental Biomedical Sciences \u201cMario Serio\u201d University of Florence Florence Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,9,26]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dei014"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/dji339"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/and.12451"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejogrb.2003.11.024"},{"key":"e_1_2_8_6_1","first-page":"163","article-title":"The comet assay. 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