{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T18:14:55Z","timestamp":1789668895311,"version":"4.0.1"},"reference-count":59,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,2,18]],"date-time":"2018-02-18T00:00:00Z","timestamp":1518912000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Science and Technology Planning Project of Guangdong Province, China","award":["2014A020213007"],"award-info":[{"award-number":["2014A020213007"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Andrology"],"published-print":{"date-parts":[[2018,3]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The human sperm nucleus contains cytoplasm. However, the origin and incidence of human sperm intranuclear cytoplasmic retention (<jats:styled-content style=\"fixed-case\">INCR<\/jats:styled-content>) remain unknown. The objectives of this study were to observe the morphological origin of <jats:styled-content style=\"fixed-case\">INCR<\/jats:styled-content> within the seminiferous epithelium and investigate the incidence of <jats:styled-content style=\"fixed-case\">INCR<\/jats:styled-content> in fertile and teratozoospermia men using transmission electron microscopy (<jats:styled-content style=\"fixed-case\">TEM<\/jats:styled-content>). By <jats:styled-content style=\"fixed-case\">TEM<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> INCR<\/jats:styled-content> initially appeared in elongating round spermatid nuclei and varied in size, number, shape, content, location and distribution within sperm nuclei. The teratozoospermia group (<jats:italic>n<\/jats:italic>\u00a0=\u00a016) demonstrated a higher incidence of <jats:styled-content style=\"fixed-case\">INCR<\/jats:styled-content> than did the fertile group (<jats:italic>n<\/jats:italic>\u00a0=\u00a016) (17.6\u00a0\u00b1\u00a05.2% vs. 9.7\u00a0\u00b1\u00a03.4%; <jats:italic>p\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.000). In the fertile group, no correlations were found between the incidence of <jats:styled-content style=\"fixed-case\">INCR<\/jats:styled-content> and abnormal sperm morphology, nuclear vacuole, acrosome integrity, motility or concentration (<jats:italic>p\u00a0<\/jats:italic>&gt;<jats:italic>\u00a0<\/jats:italic>0.05). However, the incidence of <jats:styled-content style=\"fixed-case\">INCR<\/jats:styled-content> exhibited a positive relationship with sperm abnormal morphology in the teratozoospermia group (<jats:italic>r\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.616, <jats:italic>p\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.011). These results demonstrate that <jats:styled-content style=\"fixed-case\">INCR<\/jats:styled-content> occurs in the early process of spermatogenesis and is an alteration found in the nucleus. Spermatozoa from teratozoospermia men contained more <jats:styled-content style=\"fixed-case\">INCR<\/jats:styled-content>s than those from fertile males. More attention should be paid to the possibility of spermatozoa containing <jats:styled-content style=\"fixed-case\">INCR<\/jats:styled-content> when using spermatozoa with abnormal head morphology for clinical or diagnostic purposes.<\/jats:p>","DOI":"10.1111\/andr.12469","type":"journal-article","created":{"date-parts":[[2018,2,19]],"date-time":"2018-02-19T01:16:51Z","timestamp":1519003011000},"page":"317-324","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Transmission electron microscopy analysis of the origin and incidence of sperm intranuclear cytoplasmic retention in fertile and teratozoospermia men"],"prefix":"10.1111","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4425-8551","authenticated-orcid":false,"given":"W.\u2010J.","family":"Zhu","sequence":"first","affiliation":[{"name":"Department of Developmental and Regenerative Biology College of Life Science and Technology Jinan University 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