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Microbiota of human semen has been studied so far mostly in case of infertility or prostatitis. We aimed to reveal possible impact of sexual debut on seminal microbiota in healthy young men. The study group included 68 young healthy men, of them 12 men without sexual experience, 11 men with single lifetime sexual partner and 45 men with multiple lifetime sexual partners. Basic semen parameters were similar for all subgroups, and no correlation between sexual experience and <jats:styled-content style=\"fixed-case\">WBC<\/jats:styled-content> counts in semen was found. A man could harbour one to nine different bacteria in his semen; the total concentration of bacteria ranged from 2.3 to 7.3 log<jats:sub>10<\/jats:sub> <jats:styled-content style=\"fixed-case\">CFU<\/jats:styled-content>\/mL of semen. Lower total bacterial concentration and lower bacterial diversity was observed in men without sexual experience than in sexually experienced men (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05), with significant positive correlation between these two parameters (<jats:italic>r\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.54; <jats:italic>p\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>0.0001). In conclusion, the sexual debut is associated with the enrichment of seminal microbiota but not with the influx of <jats:styled-content style=\"fixed-case\">WBC<\/jats:styled-content> or changes in basic seminal parameters.<\/jats:p>","DOI":"10.1111\/andr.12482","type":"journal-article","created":{"date-parts":[[2018,3,7]],"date-time":"2018-03-07T06:38:37Z","timestamp":1520404717000},"page":"510-512","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["Impact of sexual debut on culturable human seminal microbiota"],"prefix":"10.1111","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9747-3618","authenticated-orcid":false,"given":"R.","family":"M\u00e4ndar","sequence":"first","affiliation":[{"name":"Department of Microbiology Institute of Biomedicine and Translational Medicine University of Tartu Tartu Estonia"},{"name":"Competence Centre on Health Technologies Tartu Estonia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"T\u00fcrk","sequence":"additional","affiliation":[{"name":"Department of Microbiology Institute of Biomedicine and Translational Medicine University of Tartu Tartu Estonia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9615-0481","authenticated-orcid":false,"given":"P.","family":"Korrovits","sequence":"additional","affiliation":[{"name":"Department of Microbiology Institute of Biomedicine and Translational Medicine University of Tartu Tartu Estonia"},{"name":"Tartu University Hospital, Centre of Andrology Tartu Estonia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Ausmees","sequence":"additional","affiliation":[{"name":"MediTA Clinic Tartu Estonia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Punab","sequence":"additional","affiliation":[{"name":"Tartu University Hospital, Centre of Andrology Tartu Estonia"},{"name":"Department of Surgery Institute of Clinical Medicine University of Tartu Tartu 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