{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T00:44:06Z","timestamp":1787013846822,"version":"build-2736575974"},"reference-count":47,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2018,3,30]],"date-time":"2018-03-30T00:00:00Z","timestamp":1522368000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["NSFC #81070484"],"award-info":[{"award-number":["NSFC #81070484"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["NSFC #81501020"],"award-info":[{"award-number":["NSFC #81501020"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["NSFC #81501246"],"award-info":[{"award-number":["NSFC #81501246"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Andrology"],"published-print":{"date-parts":[[2018,5]]},"abstract":"Summary<\/jats:title>\n \n Adipose\u2010derived stem cells (\n ADSC<\/jats:styled-content>\n s) have recently been considered as a promising therapy for erectile dysfunction (\n ED<\/jats:styled-content>\n ). However, the mechanism of\n ADSC<\/jats:styled-content>\n \u2010based therapy is unclear. Insulin\u2010like growth factor\u20101 (\n IGF<\/jats:styled-content>\n \u20101), basic fibroblast growth factor (\n bFGF<\/jats:styled-content>\n ), and vascular endothelial growth factor (\n VEGF<\/jats:styled-content>\n ) secreted by\n ADSC<\/jats:styled-content>\n s were assessed in\u00a0vitro. Sixteen 24\u2010month\u2010old male Sprague\u2013Dawley rats were used for comparative analysis of 2\u2010week treatment with labeled\n ADSC<\/jats:styled-content>\n s or\n PBS<\/jats:styled-content>\n . Eight additional 5\u2010month\u2010old rats were used as a young rat group. At 2\u00a0weeks post\u2010transplantation, all rats were analyzed for erectile function, cavernous\n IGF<\/jats:styled-content>\n \u20101,\n bFGF<\/jats:styled-content>\n and\n VEGF<\/jats:styled-content>\n levels, and penile histology. Conditioned medium and co\u2010culture systems were used in cell experiments to detect how growth factors act on corpus cavernosum smooth muscle cells (\n CCSMC<\/jats:styled-content>\n s) under oxidative stress conditions via crystal violet staining and immunofluorescence staining. We found that\n ADSC<\/jats:styled-content>\n s secreted significantly higher\n IGF<\/jats:styled-content>\n \u20101,\n bFGF<\/jats:styled-content>\n , and\n VEGF<\/jats:styled-content>\n levels in culture medium compared with basal medium. Compared with young rats, untreated aged rats had significantly lower Max\n ICP<\/jats:styled-content>\n \/\n MAP<\/jats:styled-content>\n and\n ADSC<\/jats:styled-content>\n treatment significantly increased the ratio. Immunofluorescence staining demonstrated a small number of labeled\n ADSC<\/jats:styled-content>\n s in the corpus cavernosum. The untreated aged rats showed significantly decreased cavernous\n IGF<\/jats:styled-content>\n \u20101,\n bFGF<\/jats:styled-content>\n , and\n VEGF<\/jats:styled-content>\n levels and significantly decreased contents of cavernous smooth muscle and endothelium compared with young rats.\n ADSC<\/jats:styled-content>\n treatment partially normalized these alterations. In cell experiments, the groups receiving growth factor neutralizing antibody separately or combined had significantly decreased numbers of\n CCSMC<\/jats:styled-content>\n s compared with control groups. These results indicated that\n ADSC<\/jats:styled-content>\n treatment may improve aging\u2010related\n ED<\/jats:styled-content>\n partially through the secretion of\n IGF<\/jats:styled-content>\n \u20101,\n bFGF<\/jats:styled-content>\n , and VEGF.\n <\/jats:p>","DOI":"10.1111\/andr.12483","type":"journal-article","created":{"date-parts":[[2018,3,30]],"date-time":"2018-03-30T20:04:51Z","timestamp":1522440291000},"page":"498-509","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":65,"title":["Adipose\u2010derived stem cells improve erectile function partially through the secretion of\n IGF<\/scp>\n \u20101,\n bFGF<\/scp>\n , and\n VEGF<\/scp>\n in aged rats"],"prefix":"10.1111","volume":"6","author":[{"given":"J.","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Urology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan China"},{"name":"Institute of Urology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Urology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan China"},{"name":"Institute of Urology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Zang","sequence":"additional","affiliation":[{"name":"Department of Urology Xuzhou City Centre Hospital Xuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Urology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan China"},{"name":"Institute of Urology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Z.","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Urology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan China"},{"name":"Institute of Urology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Urology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan China"},{"name":"Institute of Urology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2909-3912","authenticated-orcid":false,"given":"Z.","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Urology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan China"},{"name":"Institute of Urology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Urology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan China"},{"name":"Institute of Urology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,3,30]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0272.2009.00977.x"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1743-6109.2010.01875.x"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.urology.2014.07.028"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.2217\/rme.09.74"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1743-6109.2009.01279.x"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00685.2006"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.ijir.3901190"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymgme.2011.11.002"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1743-6109.2011.02412.x"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11010-013-1855-8"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsxm.2017.07.005"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1743-6109.2011.02505.x"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2017.4064"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2011.07.061"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.21037\/tau.2016.11.18"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1743-6109.2009.01541.x"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000265074.83288.09"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/aja.2010.116"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1743-6109.2009.01624.x"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrurol.2012.143"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2013.05.036"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/stem.789"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1743-6109.2009.01697.x"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(05)67900-1"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2013.0495"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1743-6109.2012.02960.x"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(05)66158-7"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1089\/scd.2010.0222"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-6143.2011.03870.x"},{"key":"e_1_2_9_31_1","doi-asserted-by":"crossref","first-page":"3027109","DOI":"10.1155\/2017\/3027109","article-title":"Basic fibroblast growth factor inhibits apoptosis and promotes proliferation of adipose\u2010derived mesenchymal stromal cells isolated from patients with type 2 diabetes by reducing cellular oxidative stress","volume":"2017","author":"Nawrocka D","year":"2017","journal-title":"Oxid Med Cell Longev"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1743-6109.2011.02469.x"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.2008.07507.x"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2004.04.032"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1745-7262.2007.00215.x"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1743-6109.2008.00817.x"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.2164\/jandrol.110.012666"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.2002.tb02246.x"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymthe.2005.10.016"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(12)60520-0"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.ju.0000149034.49289.2b"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2605.2012.01250.x"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1089\/10763270360696941"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1743-6109.2009.01648.x"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.12346"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2047-2927.2012.00060.x"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1743-6109.2010.02128.x"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1111\/and.12871"}],"container-title":["Andrology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fandr.12483","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/andr.12483","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,3]],"date-time":"2025-07-03T03:37:14Z","timestamp":1751513834000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/andr.12483"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,3,30]]},"references-count":47,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2018,5]]}},"alternative-id":["10.1111\/andr.12483"],"URL":"https:\/\/doi.org\/10.1111\/andr.12483","archive":["Portico"],"relation":{},"ISSN":["2047-2919","2047-2927"],"issn-type":[{"value":"2047-2919","type":"print"},{"value":"2047-2927","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,3,30]]},"assertion":[{"value":"2017-09-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-02-14","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-03-30","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}