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Several factors are involved in recognition of the correct 3\u2032\u2010end site. The\n                    <jats:styled-content>c<\/jats:styled-content>\n                    leavage\n                    <jats:styled-content>st<\/jats:styled-content>\n                    imulation\n                    <jats:styled-content>f<\/jats:styled-content>\n                    actor (CstF) has three subunits, CstF\u201050 (gene symbol\n                    <jats:italic>Cstf1<\/jats:italic>\n                    ), CstF\u201064\n                    <jats:italic>(Cstf2)<\/jats:italic>\n                    , and CstF\u201077\n                    <jats:italic>(Cstf3)<\/jats:italic>\n                    . Of these, CstF\u201064 is the\n                    <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\n                    \u2010binding subunit that interacts with the pre\u2010\n                    <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content>\n                    downstream of the cleavage site. In male germ cells where CstF\u201064 is not expressed, a paralog, \u03c4CstF\u201064 (gene symbol\n                    <jats:italic>Cstf2t<\/jats:italic>\n                    ) assumes its functions. Accordingly,\n                    <jats:italic>Cstf2t<\/jats:italic>\n                    knockout (\n                    <jats:italic>Cstf2t<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>\u2212\/\u2212<\/jats:italic>\n                    <\/jats:sup>\n                    ) mice exhibit male infertility due to defective development of spermatocytes and spermatids. To discover differentially expressed genes responsive to \u03c4CstF\u201064, we performed\n                    <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\n                    \u2010Seq in seminiferous tubules from wild\u2010type and\n                    <jats:italic>Cstf2t<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>\u2212\/\u2212<\/jats:italic>\n                    <\/jats:sup>\n                    mice, and found that several histone and histone\u2010like\n                    <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content>\n                    s were reduced in\n                    <jats:italic>Cstf2t<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>\u2212\/\u2212<\/jats:italic>\n                    <\/jats:sup>\n                    mice. We further observed delayed accumulation of the testis\u2010specific histone, H1fnt (formerly, H1t2 or Hanp1) in\n                    <jats:italic>Cstf2t<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>\u2212\/\u2212<\/jats:italic>\n                    <\/jats:sup>\n                    mice. High\u2010throughput sequence analysis of polyadenylation sites (A\u2010seq) indicated reduced use of polyadenylation sites within a cluster downstream of\n                    <jats:italic>H1fnt<\/jats:italic>\n                    in knockout mice. However, high\u2010throughput sequencing of\n                    <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\n                    isolated by cross\u2010linking immunoprecipitation (\n                    <jats:styled-content style=\"fixed-case\">HITS<\/jats:styled-content>\n                    \u2010\n                    <jats:styled-content style=\"fixed-case\">CLIP<\/jats:styled-content>\n                    ) was not consistent with a direct role of \u03c4CstF\u201064 in polyadenylation of\n                    <jats:italic>H1fnt<\/jats:italic>\n                    . These findings together suggest that the \u03c4CstF\u201064 may control other reproductive functions that are not directly linked to the formation of 3\u2032 ends of mature polyadenylated\n                    <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content>\n                    s during male germ cell formation.\n                  <\/jats:p>","DOI":"10.1111\/andr.12488","type":"journal-article","created":{"date-parts":[[2018,4,19]],"date-time":"2018-04-19T15:10:54Z","timestamp":1524150654000},"page":"605-615","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["<i>Cstf2t<\/i>\n                    Regulates expression of histones and histone\u2010like proteins in male germ cells"],"prefix":"10.1111","volume":"6","author":[{"given":"P. N.","family":"Grozdanov","sequence":"first","affiliation":[{"name":"Department of Cell Biology &amp; Biochemistry Texas Tech University Health Sciences Center Lubbock TX USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Electrical and Computer Engineering &amp; TEES\u2010AgriLife Center for Bioinformatics and Genomic Systems Engineering Texas A&amp;M University College Station TX USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Electrical and Computer Engineering &amp; TEES\u2010AgriLife Center for Bioinformatics and Genomic Systems Engineering Texas A&amp;M University College Station TX USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W.","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Physiology and Cell Biology University of Nevada Reno School of Medicine Reno NV USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3209-1982","authenticated-orcid":false,"given":"C. 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