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With this purpose, spermatozoa were incubated in a specifically designed\n                    <jats:italic>in\u00a0vitro<\/jats:italic>\n                    capacitation medium (CM) in the presence or absence of reduced glutathione (GSH). Incubation of boar spermatozoa in CM for 4\u00a0h significantly (\n                    <jats:italic>p\u00a0<\/jats:italic>\n                    &lt;\n                    <jats:italic>\u00a0<\/jats:italic>\n                    0.05) increased free cysteine residues, which is a marker of disrupted disulphide bonds, and also intracellular ROS levels. The addition of GSH to the medium prevented most capacitation\u2010like changes in sperm motility, membrane lipid disorder, mitochondrial membrane potential, intracellular calcium levels and localization of tyrosine\u2010phosphorylated proteins (pTyr), but not in tyrosine phosphorylation of P32. These effects were accompanied by the inhibition of the ability of sperm cells to trigger the acrosome exocytosis in response to progesterone. When GSH was added together with progesterone after 4\u00a0h of incubation, acrosome exocytosis was not altered, but the subsequent decrease in intracellular calcium observed in controls cells was inhibited. Furthermore, co\u2010incubation of oocytes with spermatozoa previously incubated in CM in the presence of GSH for 4\u00a0h significantly (\n                    <jats:italic>p\u00a0<\/jats:italic>\n                    &lt;\n                    <jats:italic>\u00a0<\/jats:italic>\n                    0.05) increased the number of spermatozoa attached to the oocyte surface but decreased normal fertilization rates. Our results suggest that boar sperm capacitation is related to an increase in disrupted disulphide bonds and intracellular ROS levels and that both events are related to the regulation of hyperactivated motility, intracellular calcium dynamics, sperm binding ability to the oocyte and achievement of proper nuclear decondensation upon oocyte penetration.\n                  <\/jats:p>","DOI":"10.1111\/andr.12514","type":"journal-article","created":{"date-parts":[[2018,8,13]],"date-time":"2018-08-13T00:55:03Z","timestamp":1534121703000},"page":"781-797","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["The achievement of boar sperm\n                    <i>in\u00a0vitro<\/i>\n                    capacitation is related to an increase of disrupted disulphide bonds and intracellular reactive oxygen species levels"],"prefix":"10.1111","volume":"6","author":[{"given":"R. P.","family":"Betarelli","sequence":"first","affiliation":[{"name":"Department of Veterinary Medicine Federal University of Lavras Lavras Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Rocco","sequence":"additional","affiliation":[{"name":"Department of Animal Medicine and Surgery Autonomous University of Barcelona Bellaterra (Cerdanyola del Vall\u00e8s) Spain"},{"name":"Department of Agriculture, Environment and Food Science University of Molise Campobasso Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2209-340X","authenticated-orcid":false,"given":"M.","family":"Yeste","sequence":"additional","affiliation":[{"name":"Department of Biology Biotechnology of Animal and Human Reproduction (TechnoSperm) Institute of Food and Agricultural Technology University of Girona Girona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. M.","family":"Fern\u00e1ndez\u2010Novell","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology University of Barcelona Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.\u00a0","family":"Placci","sequence":"additional","affiliation":[{"name":"Department of Animal Medicine and Surgery Autonomous University of Barcelona Bellaterra (Cerdanyola del Vall\u00e8s) Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Azevedo Pereira","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine Federal University of Lavras Lavras Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Castillo\u2010Mart\u00edn","sequence":"additional","affiliation":[{"name":"Department of Biology Biotechnology of Animal and Human Reproduction (TechnoSperm) Institute of Food and Agricultural Technology University of Girona Girona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Estrada","sequence":"additional","affiliation":[{"name":"Department of Animal Medicine and Surgery Autonomous University of Barcelona Bellaterra (Cerdanyola del Vall\u00e8s) Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Pe\u00f1a","sequence":"additional","affiliation":[{"name":"Department of Animal Medicine and Surgery Autonomous University of Barcelona Bellaterra (Cerdanyola del Vall\u00e8s) Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3530-9345","authenticated-orcid":false,"given":"M. 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