{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T10:54:35Z","timestamp":1791975275368,"version":"4.4.0"},"reference-count":41,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2018,7,25]],"date-time":"2018-07-25T00:00:00Z","timestamp":1532476800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["21580367"],"award-info":[{"award-number":["21580367"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Andrology"],"published-print":{"date-parts":[[2019,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Ephrin receptors (Eph) and ligands are membrane\u2010bound cell\u2013cell communication molecules that regulate the spatial organization of various tissues and organs by repulsive or adhesive signals arising from contact between Eph\u2010 and ephrin\u2010bearing cells. However, the expression and functions of Eph receptors in the testis and epididymis are virtually unknown.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>We aimed to investigate the expression of several EphB receptors and ephrin\u2010B ligands in the testis and epididymis of adult mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>mRNA and protein expression was detected via reverse transcription\u2010polymerase chain reaction amplification and immunostaining, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Complementary expression patterns were observed in the epithelia along the excurrent duct system in the testis and epididymis; ephrin\u2010B1 was strongly expressed in the epithelia of the rete testis and segment I in the ductus epididymis, whereas EphB2 and\/or EphB4 were strongly expressed in the epithelia of the straight tubules and efferent ductules. Moreover, ephrin\u2010B1 was expressed in the spermatogonia, Leydig cells, and peritubular myoid cells in the testis, whereas EphB2 was expressed in elongated spermatids and EphB4 was expressed in the spermatogonia and Leydig cells. Furthermore, these receptors were found to be tyrosine\u2010phosphorylated in the testis and\/or epididymis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p>Receptor localization and phosphorylation patterns suggested that EphB\/ephrin\u2010B signaling might occur in the seminiferous tubules and epithelial junctions among the straight tubules, rete testis, efferent ductules, and ductus epididymis. Therefore, we propose that EphB\/ephrin\u2010B signaling may regulate epithelial boundary formation in the excurrent tubule\/ductule\/duct system as well as modulate spermatogenesis and spermiation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Overall, this study represents the first analysis of EphB receptor and ephrin\u2010B ligand expression in the normal adult testis and epididymis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/andr.12523","type":"journal-article","created":{"date-parts":[[2018,7,25]],"date-time":"2018-07-25T07:10:59Z","timestamp":1532502659000},"page":"888-901","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Compartments with predominant ephrin\u2010B1 and EphB2\/B4 expression are present alternately along the excurrent duct system in the adult mouse testis and epididymis"],"prefix":"10.1111","volume":"7","author":[{"given":"M. R.","family":"Gofur","sequence":"first","affiliation":[{"name":"Laboratory of Veterinary Anatomy Graduate School of Life and Environmental Sciences Osaka Prefecture University Izumisano Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1479-8706","authenticated-orcid":false,"given":"K.","family":"Ogawa","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Anatomy Graduate School of Life and Environmental Sciences Osaka Prefecture University Izumisano 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