{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T09:52:42Z","timestamp":1777542762747,"version":"3.51.4"},"reference-count":36,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2018,8,12]],"date-time":"2018-08-12T00:00:00Z","timestamp":1534032000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100007335","name":"Ministry of Higher Education and Scientific Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007335","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Andrology"],"published-print":{"date-parts":[[2018,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>No study has assessed the possible involvement\u00a0of endothelial nitric oxide synthase (<jats:italic><jats:styled-content style=\"fixed-case\">eNOS<\/jats:styled-content><\/jats:italic>) T\u2010786C and G894T and G\u2010protein \u03b23 subunit (<jats:italic><jats:styled-content style=\"fixed-case\">GNB<\/jats:styled-content>3<\/jats:italic>) C825T polymorphisms with susceptibility to diabetic vasculogenic erectile dysfunction (<jats:styled-content style=\"fixed-case\">VED<\/jats:styled-content>) in North African subjects.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>Our aim was to evaluate the interaction and association between these gene polymorphisms and this disorder.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>A total of 164 type 2 diabetes patients with <jats:styled-content style=\"fixed-case\">VED<\/jats:styled-content> diagnosed with penile color Doppler ultrasonography and 148 age\u2010matched healthy volunteers were genotyped for the rs1799983 (G894T) and rs2070744 (T\u2010786C) of the <jats:italic><jats:styled-content style=\"fixed-case\">eNOS<\/jats:styled-content><\/jats:italic> gene and the rs5443 (C825T) of the <jats:italic><jats:styled-content style=\"fixed-case\">GNB<\/jats:styled-content>3<\/jats:italic> gene using the <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">RFLP<\/jats:styled-content> method.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A significant association of the <jats:italic><jats:styled-content style=\"fixed-case\">eNOS<\/jats:styled-content><\/jats:italic> G894T (<jats:italic>p\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.005) and T\u2010786C (<jats:italic>p\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.02) with altered susceptibility to <jats:styled-content style=\"fixed-case\">VED<\/jats:styled-content> was observed. The risk also holds for the G894T and T\u2010786C <jats:italic><jats:styled-content style=\"fixed-case\">eNOS<\/jats:styled-content><\/jats:italic> gene polymorphisms when excluding patients with dyslipidemia and cardiovascular diseases (<jats:italic>p\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>1.7\u00b710<jats:sup>\u22124<\/jats:sup> and <jats:italic>p\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>3.2\u00b710<jats:sup>\u22125<\/jats:sup>, respectively). The univariate odds ratio associated with <jats:styled-content style=\"fixed-case\">CC<\/jats:styled-content> alleles of the <jats:italic><jats:styled-content style=\"fixed-case\">eNOS<\/jats:styled-content><\/jats:italic> T\u2010786C revealed a four times increased risk for <jats:styled-content style=\"fixed-case\">VED<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">OR<\/jats:styled-content>\u00a0=\u00a04.04; 95% <jats:styled-content style=\"fixed-case\">CI<\/jats:styled-content>\u00a0=\u00a01.53\u201310.67;\u00a0<jats:italic>p\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.006). <jats:styled-content style=\"fixed-case\">VED<\/jats:styled-content> risk was also associated with the G894T variant under dominant model (<jats:italic>p\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.002) and the T\u2010786C variant under recessive model (<jats:italic>p\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.004). Furthermore, the concomitant presence of the combined genotypes of the 894T and 786T strongly affected the predisposition to <jats:styled-content style=\"fixed-case\">VED<\/jats:styled-content> (<jats:italic>p\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.007).<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion and Conclusion<\/jats:title><jats:p>Our study gave a comprehensive insight into functional interaction between <jats:italic><jats:styled-content style=\"fixed-case\">GNB<\/jats:styled-content>3<\/jats:italic> and <jats:italic><jats:styled-content style=\"fixed-case\">eNOS<\/jats:styled-content><\/jats:italic> gene polymorphisms and suggests that the <jats:italic><jats:styled-content style=\"fixed-case\">eNOS<\/jats:styled-content><\/jats:italic> G894T and T\u2010786C variants are strong predisposing factors of <jats:styled-content style=\"fixed-case\">VED<\/jats:styled-content> susceptibility within men with type 2 diabetes.<\/jats:p><\/jats:sec>","DOI":"10.1111\/andr.12543","type":"journal-article","created":{"date-parts":[[2018,8,13]],"date-time":"2018-08-13T05:14:50Z","timestamp":1534137290000},"page":"865-873","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Comprehensive insight into functional interaction between GNB3 C825T and eNOS T\u2010786C, G894T gene polymorphisms and association with susceptibility to diabetic erectile dysfunction"],"prefix":"10.1111","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1527-7582","authenticated-orcid":false,"given":"M. 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