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However, until now the role of this musculature was unknown.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>(i) To characterize the Bu\u2010Gs striated muscular layer in male rats and determine its innervation and response to genital stimulation. (ii) To reveal the role of the Bu\u2010Gs striated musculature in the release of glandular secretion. (iii) To elucidate the effect of bilateral ablation of the Bu\u2010Gs on copulatory behavior and seminal fluid characteristics.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>Adult Wistar male rats were allocated in three experiments: in Experiment 1, the Bu\u2010Gs striated musculature, innervation and reflex activity were determined by gross anatomy and histological and electrophysiological techniques; electromyographic activity of the Bu\u2010Gs striated musculature was evoked with genital stimulation. In Experiment 2, Bu\u2010Gs were analyzed after copulatory behavior of intact or animals with unilateral transected motor branch of the sacral plexus (<jats:styled-content style=\"fixed-case\">MBSP<\/jats:styled-content>). In Experiment 3, copulatory behavior and spermatobioscopy of males with bilateral ablation of the Bu\u2010Gs or sham surgery were analyzed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The Bu\u2010Gs striated fibers discharged in response to mechanostimulation of the prepuce, glans, and penile\u2010urethra. Innervation of the Bu\u2010Gs striated musculature originated from the <jats:styled-content style=\"fixed-case\">MBSP<\/jats:styled-content>; this nerve also innervates striated penile muscles. Unilateral transection of the <jats:styled-content style=\"fixed-case\">MBSP<\/jats:styled-content> significantly decreased the secretion from the ipsilateral Bu\u2010G to the nerve transection. Bilateral ablation of Bu\u2010Gs did not affect seminal plug formation but decreased semen viscosity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion and conclusion<\/jats:title><jats:p>The Bu\u2010Gs striated musculature contributes to expel glandular secretion during sexual intercourse. The somatic control of Bu\u2010Gs secretion is additional to the reported autonomic innervation supplied by the cavernosus nerve, which may underlie the synthesis of secretion as well as contraction of Bu\u2010Gs smooth muscle.<\/jats:p><\/jats:sec>","DOI":"10.1111\/andr.12549","type":"journal-article","created":{"date-parts":[[2018,9,19]],"date-time":"2018-09-19T12:14:42Z","timestamp":1537359282000},"page":"102-109","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Somatic innervation contributes to the release of bulbourethral gland secretion in male rats"],"prefix":"10.1111","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2964-0995","authenticated-orcid":false,"given":"J. 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