{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T14:55:50Z","timestamp":1781967350508,"version":"3.54.5"},"reference-count":56,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,11,23]],"date-time":"2018-11-23T00:00:00Z","timestamp":1542931200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100009633","name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development","doi-asserted-by":"publisher","award":["P01 HD075795"],"award-info":[{"award-number":["P01 HD075795"]}],"id":[{"id":"10.13039\/100009633","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["P30 CA 016672"],"award-info":[{"award-number":["P30 CA 016672"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Andrology"],"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Cytotoxic cancer treatments, such as irradiation, can cause permanent sterility in male mammals owing to the loss of spermatogonial stem cells. In animal models, spermatogenesis could be restored from transplanted spermatogonial stem cells. Previously, we showed that transient suppression of FSH, LH, and testosterone in the recipient with a gonadotropin\u2010releasing hormone antagonist (Gn<jats:styled-content style=\"fixed-case\">RH<\/jats:styled-content>\u2010ant), given immediately after irradiation, enhanced spermatogenesis from transplanted spermatogonial stem cells in mice and monkeys.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To explore improvements in the preparation of the recipient for efficient and reliable spermatogenic recovery from spermatogonial stem cell transplantation, so that it can be used effectively in clinical practice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>In mouse recipients, we evaluated the effects of hormone suppression given after germ cell depletion was complete, which is a more clinically relevant model, and also the importance of total androgen ablation and maintenance of <jats:styled-content style=\"fixed-case\">FSH<\/jats:styled-content> levels. Three regimens, Gn<jats:styled-content style=\"fixed-case\">RH<\/jats:styled-content>\u2010ant, Gn<jats:styled-content style=\"fixed-case\">RH<\/jats:styled-content>\u2010ant plus flutamide (androgen receptor antagonist), and Gn<jats:styled-content style=\"fixed-case\">RH<\/jats:styled-content>\u2010ant plus <jats:styled-content style=\"fixed-case\">FSH<\/jats:styled-content>, were administered prior to and around the time of transplantation of testis cells from immature mice or from prepubertal monkeys.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Treatment with Gn<jats:styled-content style=\"fixed-case\">RH<\/jats:styled-content>\u2010ant resulted in a fourfold increase in spermatogenic recovery from <jats:styled-content style=\"fixed-case\">GFP<\/jats:styled-content>\u2010marked transplanted mouse cells. Total androgen ablation with the addition of flutamide, started two weeks before transplantation, did not further enhance recovery. Surprisingly, <jats:styled-content style=\"fixed-case\">FSH<\/jats:styled-content> supplementation, started around the time of transplantation, actually reduced spermatogenic recovery from transplanted spermatogonial stem cells in Gn<jats:styled-content style=\"fixed-case\">RH<\/jats:styled-content>\u2010ant\u2010treated mice. When prepubertal monkey testicular cells were transplanted into nude mice that were given the same hormone treatments, the numbers of donor\u2010derived colonies were independent of hormone treatment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion and Conclusion<\/jats:title><jats:p>The enhancements in spermatogenic recovery may only occur when syngeneic or closely related donor\u2013recipient pairs are used. These results are useful in further investigations in choosing a hormone suppression regimen in combination with spermatogonial transplantation as a treatment to restore fertility in primates after cytotoxic therapy.<\/jats:p><\/jats:sec>","DOI":"10.1111\/andr.12571","type":"journal-article","created":{"date-parts":[[2018,11,24]],"date-time":"2018-11-24T14:59:59Z","timestamp":1543071599000},"page":"257-265","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Effect of hormone modulations on donor\u2010derived spermatogenesis or colonization after syngeneic and xenotransplantation in mice"],"prefix":"10.1111","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5786-0667","authenticated-orcid":false,"given":"G.","family":"Shetty","sequence":"first","affiliation":[{"name":"Department of Experimental Radiation Oncology The University of Texas MD Anderson Cancer Center Houston TX USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Z.","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Experimental Radiation Oncology The University of Texas MD Anderson Cancer Center Houston TX USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T. N. A.","family":"Lam","sequence":"additional","affiliation":[{"name":"Department of Experimental Radiation Oncology The University of Texas MD Anderson Cancer Center Houston TX USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T. T.","family":"Phan","sequence":"additional","affiliation":[{"name":"Department of Experimental Radiation Oncology The University of Texas MD Anderson Cancer Center Houston TX USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K. E.","family":"Orwig","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, Gynecology and Reproductive Sciences Magee\u2010Womens Research Institute University of Pittsburgh School of Medicine Pittsburgh PA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. 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