{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T00:44:06Z","timestamp":1787013846585,"version":"build-2736575974"},"reference-count":31,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,12,21]],"date-time":"2018-12-21T00:00:00Z","timestamp":1545350400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003453","name":"Natural Science Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["No.2016A030310393"],"award-info":[{"award-number":["No.2016A030310393"]}],"id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010096","name":"Southern Medical University","doi-asserted-by":"publisher","award":["PY2016N018"],"award-info":[{"award-number":["PY2016N018"]}],"id":[{"id":"10.13039\/501100010096","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Andrology"],"published-print":{"date-parts":[[2019,3]]},"abstract":"Abstract<\/jats:title>\n \n Background<\/jats:title>\n \n Semenogelin 1 (\n \n SEMG<\/jats:styled-content>\n 1\n <\/jats:italic>\n ) is an important secretory protein in spermatozoa involved in the formation of a gel matrix encasing ejaculated spermatozoa. Previous studies show that the\n SEMG<\/jats:styled-content>\n 1 gene is highly expressed in spermatozoa from patients with asthenozoospermia (\n AZS<\/jats:styled-content>\n ); however, the underlying molecular mechanisms are not yet clear.\n <\/jats:p>\n <\/jats:sec>\n \n Objectives<\/jats:title>\n \n To study the molecular mechanism of high expression of\n SEMG<\/jats:styled-content>\n 1 gene and its potential roles in\n AZS<\/jats:styled-content>\n .\n <\/jats:p>\n <\/jats:sec>\n \n Materials and Methods<\/jats:title>\n \n Western blot and real\u2010time\n PCR<\/jats:styled-content>\n were used to detect the expression levels of\n \n SEMG<\/jats:styled-content>\n 1\n <\/jats:italic>\n protein and\n mRNA<\/jats:styled-content>\n in the ejaculated spermatozoa from normozoospermic males and\n AZS<\/jats:styled-content>\n patients. Bioinformatics analysis was used to predict mi\n RNA<\/jats:styled-content>\n s targeting for\n SEMG<\/jats:styled-content>\n 1 3\u2032\u2010untranslated region detection of the expression levels of all the candidate mi\n RNA<\/jats:styled-content>\n s in ejaculatory spermatozoa in\n AZS<\/jats:styled-content>\n patients or normozoospermic volunteers. Luciferase reporter assays were performed to confirm it can directly bind to\n SEMG<\/jats:styled-content>\n 1. Correlation of miR\u2010525\u20103p and\n \n SEMG<\/jats:styled-content>\n 1\n <\/jats:italic>\n mRNA<\/jats:styled-content>\n expression with clinical sperm parameters were also analyzed. Finally, we conducted a follow\u2010up study of reproductive history about all the subjects.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n SEMG<\/jats:styled-content>\n 1\n mRNA<\/jats:styled-content>\n and protein level were significantly higher in\n AZS<\/jats:styled-content>\n patients compared to that in normozoospermic volunteers (\n p\u00a0<\/jats:italic>\n <\n \u00a0<\/jats:italic>\n 0.001). Subsequently, micro\n RNA<\/jats:styled-content>\n \u2010525\u20103p (miR\u2010525\u20103p) which was predicted as a candidate regulator of\n \n SEMG<\/jats:styled-content>\n 1\n <\/jats:italic>\n was found lower expressed in ejaculatory spermatozoa in\n AZS<\/jats:styled-content>\n patients (\n p<\/jats:italic>\n \u00a0=\u00a00.0074). Luciferase experiment revealed that micro\n RNA<\/jats:styled-content>\n \u2010525\u20103p could directly target\n \n SEMG<\/jats:styled-content>\n 1\n <\/jats:italic>\n 3\u2032\u2010untranslated region and suppress its expression. Importantly, our retrospective follow\u2010up study showed that both low miR\u2010525\u20103p expression and high\n SEMG<\/jats:styled-content>\n 1 expression level was significantly associated with low progressive sperm motility, abnormal sperm morphology, and infertility.\n <\/jats:p>\n <\/jats:sec>\n \n Discussion and Conclusion<\/jats:title>\n \n The elevated expression of\n SEMG<\/jats:styled-content>\n 1 and reduced expression of miR\u2010525\u20103p are associated with\n AZS<\/jats:styled-content>\n and male infertility. Our study provides a potential therapeutic target for the treatment of male infertility or for male contraception.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/andr.12573","type":"journal-article","created":{"date-parts":[[2018,12,21]],"date-time":"2018-12-21T11:24:05Z","timestamp":1545391445000},"page":"220-227","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Expressions of miR\u2010525\u20103p and its target gene\n \n SEMG<\/scp>\n 1\n <\/i>\n in the spermatozoa of patients with asthenozoospermia"],"prefix":"10.1111","volume":"7","author":[{"given":"Q.\u2010z.","family":"Zhou","sequence":"first","affiliation":[{"name":"Department of Urology The Third Affiliated Hospital of Southern Medical University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"X.\u2010b.","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Urology The Third Affiliated Hospital of Southern Medical University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W.\u2010s.","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Urology The Third Affiliated Hospital of Southern Medical University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.\u2010h.","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Urology The Third Affiliated Hospital of Southern Medical University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Urology The Third Affiliated Hospital of Southern Medical University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Bian","sequence":"additional","affiliation":[{"name":"Department of Urology The Third Affiliated Hospital of Southern Medical University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.\u2010k.","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Urology The Third Affiliated Hospital of Southern Medical University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W.\u2010b.","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Urology The Third Affiliated Hospital of Southern Medical University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Urology The Third Affiliated Hospital of Southern Medical University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Qi","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Nanfang Hospital Southern Medical University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.\u2010y.","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Nanfang Hospital Southern Medical University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.\u2010k.","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Urology The Third Affiliated Hospital of Southern Medical University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2098-1139","authenticated-orcid":false,"given":"C.\u2010d.","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Urology The Third Affiliated Hospital of Southern Medical University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,12,21]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/andr.12280"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1089\/omi.2016.0173"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2005.06.002"},{"key":"e_1_2_9_5_1","doi-asserted-by":"crossref","unstructured":"DongFN Amiri\u2010YektaA MartinezG SautA TekJ StouvenelL LoresP KaraouzeneT Thierry\u2010MiegN SatreV BrouilletS DaneshipourA HosseiniSH BonhiversM GourabiH DulioustE ArnoultC ToureA RayPF ZhaoH&CouttonC. 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