{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T09:32:51Z","timestamp":1768728771602,"version":"3.49.0"},"reference-count":61,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2019,6,4]],"date-time":"2019-06-04T00:00:00Z","timestamp":1559606400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100012681","name":"College of Natural Resources and Sciences, Humboldt State University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100012681","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001677","name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001677","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010045","name":"University for the Creative Arts","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010045","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Andrology"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Lipid metabolic disorders (dyslipidemia) are constantly increasing in occidental societies and lead to the development of pathologies such as obesity, diabetes, and metabolic syndrome. It has been demonstrated that dyslipidemia can alter the reproductive function. Animal models have recently been used to show that the offspring of dyslipidemic males could also develop such pathologies and that the transgenerational transmission involved post\u2010testicular sperm maturation. These data targeted the essential role of male gamete epididymal maturation and its importance for the health of the offspring.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>This publication summarizes in the first place experimental data obtained using a mouse model of dyslipidemia\u2010induced post\u2010testicular infertility, knockout mice for the two isoforms of the \u2018Liver X Receptors\u2019 (<jats:italic>Lxr<\/jats:italic>\u03b1<jats:italic>;<\/jats:italic>\u03b2<jats:sup><jats:italic>\u2212\/\u2212<\/jats:italic><\/jats:sup>), the major regulators of cholesterol homeostasis. The impact of a high cholesterol diet (<jats:styled-content style=\"fixed-case\">HCD<\/jats:styled-content>) on the protein <jats:styled-content style=\"fixed-case\">YWHAZ<\/jats:styled-content> (14\u20103\u20103 \u03b6 or tyrosine 3\u2010monooxygenase\/tryptophan 5\u2010monooxygenase activation protein Zeta) was also investigated in our model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>In our mouse model, when young fertile <jats:italic>Lxr<\/jats:italic>\u03b1<jats:italic>;<\/jats:italic>\u03b2<jats:sup><jats:italic>\u2212\/\u2212<\/jats:italic><\/jats:sup> males aged three months were fed four weeks with a <jats:styled-content style=\"fixed-case\">HCD<\/jats:styled-content>, they developed an epididymal phenotype leading to infertility. The level of sperm <jats:styled-content style=\"fixed-case\">YWHAZ<\/jats:styled-content> was evaluated by Western blot and its tyrosine phosphorylation state by immunoprecipitation followed by Western blot.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Our data revealed that sperm lipid composition and structure were altered, leading to defects of the capacitation\u2010associated signaling pathway. They also showed that both the level and the tyrosine phosphorylation state of <jats:styled-content style=\"fixed-case\">YWHAZ<\/jats:styled-content> were affected by the <jats:styled-content style=\"fixed-case\">HCD<\/jats:styled-content> in sperm cells from <jats:italic>Lxr<\/jats:italic>\u03b1<jats:italic>;<\/jats:italic>\u03b2<jats:sup><jats:italic>\u2212\/\u2212<\/jats:italic><\/jats:sup> males.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion and conclusion<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">YWHAZ<\/jats:styled-content> could be a new important regulator of capacitation\u2010associated tyrosine phosphorylation and a marker of dyslipidemia\u2010induced infertility.<\/jats:p><\/jats:sec>","DOI":"10.1111\/andr.12634","type":"journal-article","created":{"date-parts":[[2019,6,4]],"date-time":"2019-06-04T10:36:51Z","timestamp":1559644611000},"page":"654-661","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Impairment of sperm maturation and capacitation due to diet\u2010dependent cholesterol overload"],"prefix":"10.1111","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9570-3777","authenticated-orcid":false,"given":"F.","family":"Saez","sequence":"first","affiliation":[{"name":"Team MEPTI (M\u00e9canismes Post\u2010Testiculaires de l'Infertilit\u00e9) GReD Laboratory Facult\u00e9 de M\u00e9decine Universit\u00e9 Clermont Auvergne CNRS, Inserm, CRBC Clermont\u2010Ferrand France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Whitfield","sequence":"additional","affiliation":[{"name":"Team MEPTI (M\u00e9canismes Post\u2010Testiculaires de l'Infertilit\u00e9) GReD Laboratory Facult\u00e9 de M\u00e9decine Universit\u00e9 Clermont Auvergne CNRS, Inserm, CRBC Clermont\u2010Ferrand France"},{"name":"Department of Development, Reproduction and Cancer INSERM U1016 \u2010 CNRS UMR 8104 \u2010 Universit\u00e9 Paris Descartes Institut Cochin Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. R.","family":"Drevet","sequence":"additional","affiliation":[{"name":"Team MEPTI (M\u00e9canismes Post\u2010Testiculaires de l'Infertilit\u00e9) GReD Laboratory Facult\u00e9 de M\u00e9decine Universit\u00e9 Clermont Auvergne CNRS, Inserm, CRBC Clermont\u2010Ferrand 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