{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T00:44:06Z","timestamp":1787013846397,"version":"build-2736575974"},"reference-count":123,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2019,5,17]],"date-time":"2019-05-17T00:00:00Z","timestamp":1558051200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["MOP 5605"],"award-info":[{"award-number":["MOP 5605"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Andrology"],"published-print":{"date-parts":[[2019,9]]},"abstract":"Abstract<\/jats:title>\n \n Background<\/jats:title>\n \n The Hermes body (\n HB<\/jats:styled-content>\n ) previously called the cytoplasmic droplet is a focal distension of the flagellar cytoplasm of epididymal spermatozoa consisting mainly of isolated flattened Golgi cisternae.\n <\/jats:p>\n <\/jats:sec>\n \n Objective<\/jats:title>\n \n To define a functional role for the\n HB<\/jats:styled-content>\n of epididymal spermatozoa.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n Isolated fractions of\n HB<\/jats:styled-content>\n s of epididymal spermatozoa were prepared and by quantitative tandem mass spectrometry revealed 1511 proteins.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n The glucose transporter\n GLUT<\/jats:styled-content>\n \u20103 was the most abundant protein followed by hexokinase 1, which along with the presence of all glycolytic enzymes suggested a role for the\n HB<\/jats:styled-content>\n in glycolysis. Several\n TMED<\/jats:styled-content>\n \/p24 Golgi trafficking proteins were abundant with\n TMED<\/jats:styled-content>\n 7\/p27 and\n TMED<\/jats:styled-content>\n 2\/p24 defining the identity of the flattened cisternae within the\n HB<\/jats:styled-content>\n as Golgi, along with the known Golgi proteins,\n GBF<\/jats:styled-content>\n 1,\n GOLPH<\/jats:styled-content>\n 3, Man2\u03b11, and Man\n IIX<\/jats:styled-content>\n . The Golgi trafficking protein\n TMED<\/jats:styled-content>\n 7\/p27 via small 50\u2010nm vesicles emanating from the Golgi cisternae was proposed to transport\n GLUT<\/jats:styled-content>\n \u20103 to the plasma membrane for\n ATP<\/jats:styled-content>\n production related to sperm motility. The internal membranes revealed abundant proteins not only of Golgi cisternae, but also of endoplasmic reticulum and endosomes.\n COPI<\/jats:styled-content>\n and\n COPII<\/jats:styled-content>\n coats, clathrin,\n SNARE<\/jats:styled-content>\n s, annexins, atlastins, and\n GTP<\/jats:styled-content>\n ases were identified for vesicular trafficking and membrane fusion, in addition to ribosomes, stress proteins for protection, proteasome proteins involved in degradation, and cytoskeletal elements for migration of the\n HB<\/jats:styled-content>\n along the flagellum. The biogenesis of the\n HB<\/jats:styled-content>\n occurring at step 19 spermatids of the testis just prior to their release was uncovered as a key step in germ cell differentiation, where several proteins were expressed, some for the first time.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n As epididymal spermatozoa undergo remodeling of their protein makeup through selective degradation of sperm proteins during epididymal transit, then remodeling as a consequence of new protein synthesis is not excluded by our observations.<\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/andr.12641","type":"journal-article","created":{"date-parts":[[2019,5,18]],"date-time":"2019-05-18T01:35:16Z","timestamp":1558143316000},"page":"566-580","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":41,"title":["Dark side of the epididymis: tails of sperm maturation"],"prefix":"10.1111","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7138-7482","authenticated-orcid":false,"given":"L.","family":"Hermo","sequence":"first","affiliation":[{"name":"Department of Anatomy and Cell Biology McGill University Montreal QC Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. 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