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Spermatozoa, cultured in the presence or absence of 10\u00a0\u03bcM H\n                      <jats:sub>2<\/jats:sub>\n                      O\n                      <jats:sub>2<\/jats:sub>\n                      , were left untreated or treated with increasing concentrations of\n                      <jats:styled-content style=\"fixed-case\">PRP<\/jats:styled-content>\n                      (2, 5, and 10%). After 24\u2010h incubation,\n                      <jats:styled-content style=\"fixed-case\">ROS<\/jats:styled-content>\n                      levels were assessed and sperm parameters were evaluated.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Our results highlight the harmful effect of H\n                      <jats:sub>2<\/jats:sub>\n                      O\n                      <jats:sub>2<\/jats:sub>\n                      on sperm parameters, showing an increase in the percentage of reactive oxygen species (\n                      <jats:styled-content style=\"fixed-case\">ROS<\/jats:styled-content>\n                      )\u2010positive cells, vacuolization, and sperm\n                      <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\n                      fragmentation, and a decrease in progressive and total motility in the H\n                      <jats:sub>2<\/jats:sub>\n                      O\n                      <jats:sub>2<\/jats:sub>\n                      \u2010treated group compared to non\u2010stressed spermatozoa. When samples were treated with\n                      <jats:styled-content style=\"fixed-case\">PRP<\/jats:styled-content>\n                      , an improvement of the studied parameters was noted mainly with 2%\n                      <jats:styled-content style=\"fixed-case\">PRP<\/jats:styled-content>\n                      , thus regarded as the best concentration to achieve a positive effect on sperm parameters. Indeed, non\u2010stressed and stressed spermatozoa treated with 2%\n                      <jats:styled-content style=\"fixed-case\">PRP<\/jats:styled-content>\n                      showed a significant increase in progressive and total motility, coupled with a decrease in\n                      <jats:styled-content style=\"fixed-case\">ROS<\/jats:styled-content>\n                      \u2010positive cells,\n                      <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\n                      fragmentation, vacuolization, and dead cells compared to the untreated group. In contrast, no significant difference in cell morphology was found between the two groups. Moreover, 2%\n                      <jats:styled-content style=\"fixed-case\">PRP<\/jats:styled-content>\n                      treatment enhanced sperm parameters and prevented cell death in H\n                      <jats:sub>2<\/jats:sub>\n                      O\n                      <jats:sub>2<\/jats:sub>\n                      \u2010exposed spermatozoa as compared to freshly collected semen.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>\n                      We suggest that\n                      <jats:styled-content style=\"fixed-case\">PRP<\/jats:styled-content>\n                      because of its wide arrays of growth factors included in his alpha granules contributes to the inhibition of\n                      <jats:styled-content style=\"fixed-case\">ROS<\/jats:styled-content>\n                      through the antioxidant, anti\u2010apoptotic activity.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Autologous\n                      <jats:styled-content style=\"fixed-case\">PRP<\/jats:styled-content>\n                      improves the quality of the sperm, more so in the presence of an H\n                      <jats:sub>2<\/jats:sub>\n                      O\n                      <jats:sub>2<\/jats:sub>\n                      \u2010induced\n                      <jats:styled-content style=\"fixed-case\">OS<\/jats:styled-content>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/andr.12648","type":"journal-article","created":{"date-parts":[[2019,5,12]],"date-time":"2019-05-12T13:24:56Z","timestamp":1557667496000},"page":"191-200","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":53,"title":["<i>In vitro<\/i>\n                    effect of autologous platelet\u2010rich plasma on H\n                    <sub>2<\/sub>\n                    O\n                    <sub>2<\/sub>\n                    \u2010induced oxidative stress in human spermatozoa"],"prefix":"10.1111","volume":"8","author":[{"given":"R.","family":"Bader","sequence":"first","affiliation":[{"name":"Mount Lebanon Hospital Azoury IVF Clinic Beirut Lebanon"},{"name":"Faculty of Medicine Regenerative Medicine and Inflammation Laboratory Saint\u2010Joseph University Beirut Lebanon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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