{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T07:21:33Z","timestamp":1773213693096,"version":"3.50.1"},"reference-count":15,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2019,7,25]],"date-time":"2019-07-25T00:00:00Z","timestamp":1564012800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100006765","name":"Chinese Medical Association","doi-asserted-by":"publisher","award":["17020410710"],"award-info":[{"award-number":["17020410710"]}],"id":[{"id":"10.13039\/501100006765","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Andrology"],"published-print":{"date-parts":[[2020,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The most common type of male infertility is asthenospermia. We cloned DnaJ heat shock protein family member B13 (<jats:italic>Dnajb13\/DNAJB13<\/jats:italic>), a type II HSP40 family member that is highly expressed in the testis. <jats:italic>DNAJB13<\/jats:italic> plays a crucial role in sperm flagellar function.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>The aim of this study was to investigate whether a correlation exists between <jats:italic>DNAJB13<\/jats:italic> and low sperm motility in infertile men.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>In the present study, we performed a mutation screening of the <jats:italic>DNAJB13<\/jats:italic> gene in 92 idiopathic asthenozoospermia patients and 200 men with normal fertility. Additionally, we used immunoelectron microscopy, co\u2010immunoprecipitation, mass spectrometric detection, indirect immunofluorescence assay, transmission electron microscopy studies, isobaric tags for relative and absolute quantitation, and multiple reaction monitoring studies to analyze changes in DNAJB13 protein.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A novel c.106T&gt;C mutation of <jats:italic>DNAJB13<\/jats:italic> was present in nearly 10% (9\/92) of idiopathic asthenozoospermia patients and was absent in 200 fertile men. A computer\u2010assisted sperm analyzer and transmission electron microscopy analysis using samples from 9 patients with <jats:italic>DNAJB13<\/jats:italic> mutations demonstrated that most spermatozoa were immotile due to sperm tail defects. Multiple reaction monitoring results indicated that DNAJB13 protein levels were reduced after gene mutation. We achieved a pregnancy rate of 100% in 8 patients with <jats:italic>DNAJB13<\/jats:italic> mutations using ICSI.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion and conclusion<\/jats:title><jats:p>The <jats:italic>DNAJB13<\/jats:italic> heterozygous variant may affect fertility. ICSI can help these patients with low fertility to father children.<\/jats:p><\/jats:sec>","DOI":"10.1111\/andr.12685","type":"journal-article","created":{"date-parts":[[2019,7,25]],"date-time":"2019-07-25T07:05:25Z","timestamp":1564038325000},"page":"299-306","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Missense mutation in <i>DNAJB13<\/i> gene correlated with male fertility in asthenozoospermia"],"prefix":"10.1111","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8728-3167","authenticated-orcid":false,"given":"W. N.","family":"Li","sequence":"first","affiliation":[{"name":"Hunan Guangxiu Hi\u2010tech Life Technology Co., Ltd. Changsha China"},{"name":"Reproductive and Genetic Hospital of CITIC\u2010Xiangya Changsha China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Zhu","sequence":"additional","affiliation":[{"name":"The Xiangtan Central Hospital Yuhu District, Xiangtan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. M.","family":"Jia","sequence":"additional","affiliation":[{"name":"Shanxi Provincial Maternal and Child Health Care Hospital Yanta District, Xi'an China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. L.","family":"Yin","sequence":"additional","affiliation":[{"name":"Reproductive and Genetic Hospital of CITIC\u2010Xiangya Changsha China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. X.","family":"Lu","sequence":"additional","affiliation":[{"name":"Reproductive and Genetic Hospital of CITIC\u2010Xiangya Changsha China"},{"name":"The Institute of Reproduction and Stem Cell Engineering Central South University Changsha China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Liu","sequence":"additional","affiliation":[{"name":"The Institute of Reproduction and Stem Cell Engineering Central South University Changsha China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,7,25]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajhg.2013.11.017"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1379\/1466-1268(1998)003<0028:SFAEOD>2.3.CO;2"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajhg.2016.06.022"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-213X-9-23"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2009.01473.x"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12861-014-0038-5"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/abbs\/36.2.93"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-4827(03)00268-4"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep31666"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-006-6192-6"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1515\/BC.2011.074"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e04-09-0787"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.02811"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200705069"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep25089"}],"container-title":["Andrology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/andr.12685","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/andr.12685","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/andr.12685","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,5]],"date-time":"2023-09-05T06:25:51Z","timestamp":1693895151000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/andr.12685"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,7,25]]},"references-count":15,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2020,3]]}},"alternative-id":["10.1111\/andr.12685"],"URL":"https:\/\/doi.org\/10.1111\/andr.12685","archive":["Portico"],"relation":{},"ISSN":["2047-2919","2047-2927"],"issn-type":[{"value":"2047-2919","type":"print"},{"value":"2047-2927","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,7,25]]},"assertion":[{"value":"2018-11-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-06-28","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-07-25","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}