{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T00:38:28Z","timestamp":1747183108040,"version":"3.40.5"},"reference-count":39,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2020,2,25]],"date-time":"2020-02-25T00:00:00Z","timestamp":1582588800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["404908"],"award-info":[{"award-number":["404908"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Andrology"],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Subcutaneous grafting\/implantation of neonatal testis tissue\/cells from diverse donor species into recipient mice can be used as an in vivo model to study testis development, spermatogenesis, and steroidogenesis. Ultrasound biomicroscopy (UBM) allows obtaining high definition cross\u2010sectional images of tissues at microscopic resolutions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>The present study was designed to (a) validate the use of UBM for non\u2010invasive monitoring of grafts\/implants overtime and to (b) correlate UBM findings with the morphological attributes of recovered grafts\/implants.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>Testis tissue fragments (~14\u00a0mm<jats:sup>3<\/jats:sup>, each) and cell aggregates (100\u00a0\u00d7\u00a010<jats:sup>6<\/jats:sup> cells, each) obtained from 1\u2010week\u2010old donor piglets (n\u00a0=\u00a030) were grafted\/implanted under the back skin of immunodeficient mice (n\u00a0=\u00a06) in eight analogous sites per mouse. Three\u2010dimensional transcutaneous Doppler UBM was performed, and a randomly selected graft and its corresponding implant were recovered at 2, 4, 6, and 8\u00a0weeks.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Graft\/implant weight (<jats:italic>P\u00a0=\u00a0<\/jats:italic>.04) and physical height (<jats:italic>P\u00a0=\u00a0<\/jats:italic>.03) increased overtime. The dynamics of physical length and volume increases over time differed between tissue grafts and cell implants (<jats:italic>P\u00a0=\u00a0<\/jats:italic>.02 and 0.01 for sample type*time interactions, respectively). UBM\u2010estimated volume was correlated with the post\u2010recovery weight and volume of the grafts\/implants (<jats:italic>r<\/jats:italic>\u00a0=\u00a00.98 and <jats:italic>r<\/jats:italic>\u00a0=\u00a00.99, respectively; <jats:italic>P\u00a0&lt;\u00a0<\/jats:italic>.001). Pre\u2010 and post\u2010recovery length and height of the grafts\/implants were positively and strongly correlated (<jats:italic>r<\/jats:italic>\u00a0=\u00a00.50, <jats:italic>P\u00a0=\u00a0<\/jats:italic>.01; <jats:italic>r<\/jats:italic>\u00a0=\u00a00.70, <jats:italic>P\u00a0=\u00a0<\/jats:italic>.001) and so were the areas covered by cordal, non\u2010cordal, or fluid\u2010filled cavities between UBM and histology (<jats:italic>r<\/jats:italic>\u00a0=\u00a00.87, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.001).<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion and conclusion<\/jats:title><jats:p>UBM findings correlated with physical attributes of the grafts\/implants, validating its use as a non\u2010invasive high\u2010fidelity tool to quantify the developmental changes in ectopic testis tissue grafts and cell implants, potentially leading to a reduction in the number of recipient mice needed for similar experiments.<\/jats:p><\/jats:sec>","DOI":"10.1111\/andr.12771","type":"journal-article","created":{"date-parts":[[2020,2,7]],"date-time":"2020-02-07T07:56:06Z","timestamp":1581062166000},"page":"1332-1346","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Validation of ultrasound biomicroscopy for the assessment of xenogeneic testis tissue grafts and cell implants in recipient mice"],"prefix":"10.1111","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0306-497X","authenticated-orcid":false,"given":"Mohammad Amin","family":"Fayaz","sequence":"first","affiliation":[{"name":"Department of Veterinary Biomedical Sciences Western College of Veterinary Medicine University of Saskatchewan Saskatoon SK Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2569-0568","authenticated-orcid":false,"given":"Awang Hazmi","family":"Awang\u2010Junaidi","sequence":"additional","affiliation":[{"name":"Department of Veterinary Biomedical Sciences Western College of Veterinary Medicine University of Saskatchewan Saskatoon SK Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6439-7831","authenticated-orcid":false,"given":"Jaswant","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Veterinary Biomedical Sciences Western College of Veterinary Medicine University of Saskatchewan Saskatoon SK Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3194-9468","authenticated-orcid":false,"given":"Ali","family":"Honaramooz","sequence":"additional","affiliation":[{"name":"Department of Veterinary Biomedical Sciences Western College of Veterinary Medicine University of Saskatchewan Saskatoon SK Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,2,25]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature00918"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.106.054999"},{"key":"e_1_2_11_4_1","unstructured":"HonaramoozA.Reproduction in domestic ruminants VIII: proceedings of the Ninth International Symposium on Reproduction in Domestic Ruminants Obihiro Hokkaido Japan August 2014. 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