{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T05:30:48Z","timestamp":1783488648718,"version":"3.55.0"},"reference-count":59,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2020,5,20]],"date-time":"2020-05-20T00:00:00Z","timestamp":1589932800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100012068","name":"Health and Care Research Wales","doi-asserted-by":"publisher","award":["HF\u201014\u201016"],"award-info":[{"award-number":["HF\u201014\u201016"]}],"id":[{"id":"10.13039\/100012068","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004919","name":"King Abdulaziz City for Science and Technology","doi-asserted-by":"publisher","award":["15\u2010MED4186\u201020"],"award-info":[{"award-number":["15\u2010MED4186\u201020"]}],"id":[{"id":"10.13039\/501100004919","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Andrology"],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Oocyte activation is driven by intracellular calcium (Ca<jats:sup>2+<\/jats:sup>) oscillations induced by sperm\u2010specific PLC\u03b6, abrogation of which causes oocyte activation deficiency in humans. Clinical PLC\u03b6 investigations have been limited to severe male infertility conditions, while PLC\u03b6 levels and localisation patterns have yet to be associated with general sperm viability.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>PLC\u03b6 profiles were examined within a general population of males attending a fertility clinic (65 patients; aged 29\u201053), examining PLC\u03b6 throughout various fractions of sperm viability. Male recruitment criteria required a minimum sperm count of 5\u00a0\u00d7\u00a010<jats:sup>6<\/jats:sup> spermatozoa\/mL, while all female patients included in this study yielded at least five oocytes for treatment. Sperm count, motility and semen volume were recorded according to standard WHO reference guidelines and correlated with PLC\u03b6 profiles examined via immunoblotting and immunofluorescence. Appropriate fertility treatments were performed following routine clinical standard operating protocols, and fertilisation success determined by successful observation of second polar body extrusion.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results and Discussion<\/jats:title><jats:p>Four distinct PLC\u03b6 patterns were observed at the equatorial, acrosomal\u00a0+\u00a0equatorial regions of the sperm head, alongside a dispersed pattern, and a population of spermatozoa without any PLC\u03b6. Acrosomal\u00a0+\u00a0equatorial PLC\u03b6 correlated most to sperm health, while dispersed PLC\u03b6 correlated to decreased sperm viability. Total levels of PLC\u03b6 exhibited significant correlations with sperm parameters. PLC\u03b6 variance corresponded to reduced sperm health, potentially underlying cases of male sub\u2010fertility and increasing male age. Finally, significantly higher levels of PLC\u03b6 were exhibited by cases of fertilisation success, alongside higher proportions of Ac\u00a0+\u00a0Eq, and lower levels of dispersed PLC\u03b6.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>PLC\u03b6 potentially represents a biomarker of sperm health, and fertilisation capacity in general cases of patients seeking fertility treatment, and not just cases of repeated fertilisation. Further focused investigations are required with larger cohorts to examine the full clinical potential of PLC\u03b6.<\/jats:p><\/jats:sec>","DOI":"10.1111\/andr.12796","type":"journal-article","created":{"date-parts":[[2020,4,16]],"date-time":"2020-04-16T20:16:07Z","timestamp":1587068167000},"page":"1143-1159","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Phospholipase C zeta profiles are indicative of optimal sperm parameters and fertilisation success in patients undergoing fertility treatment"],"prefix":"10.1111","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5056-8222","authenticated-orcid":false,"given":"Junaid","family":"Kashir","sequence":"first","affiliation":[{"name":"College of Medicine Alfaisal University Riyadh Kingdom of Saudi Arabia"},{"name":"School of Biosciences Cardiff University Cardiff UK"},{"name":"Department of Comparative Medicine King Faisal Specialist Hospital and Research Center Riyadh Kingdom of Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bhavesh V.","family":"Mistry","sequence":"additional","affiliation":[{"name":"Department of Comparative Medicine King Faisal Specialist Hospital and Research Center Riyadh Kingdom of Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lujain","family":"BuSaleh","sequence":"additional","affiliation":[{"name":"College of Medicine Alfaisal University Riyadh Kingdom of Saudi Arabia"},{"name":"Department of Comparative Medicine King Faisal Specialist Hospital and Research Center Riyadh Kingdom of Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reema","family":"Abu\u2010Dawas","sequence":"additional","affiliation":[{"name":"College of Medicine Alfaisal University Riyadh Kingdom of Saudi Arabia"},{"name":"Department of Comparative Medicine King Faisal Specialist Hospital and Research Center Riyadh Kingdom of Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michail","family":"Nomikos","sequence":"additional","affiliation":[{"name":"College of Medicine QU Health Qatar University Doha Qatar"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed","family":"Ajlan","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine King Faisal Specialist Hospital and Research Centre Riyadh Kingdom of Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raed","family":"Abu\u2010Dawud","sequence":"additional","affiliation":[{"name":"Department of Comparative Medicine King Faisal Specialist Hospital and Research Center Riyadh Kingdom of Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadya","family":"AlYacoub","sequence":"additional","affiliation":[{"name":"Department of Comparative Medicine King Faisal Specialist Hospital and Research Center Riyadh Kingdom of Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saad","family":"AlHassan","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine King Faisal Specialist Hospital and Research Centre Riyadh Kingdom of Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F. 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