{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T14:38:00Z","timestamp":1788964680087,"version":"build-2803163510"},"reference-count":30,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2020,6,8]],"date-time":"2020-06-08T00:00:00Z","timestamp":1591574400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Andrology"],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Recently, alterations in miRNAs expression profile in semen have been linked to damaged spermatogenesis, suggesting miRNAs could be used as potential infertility biomarkers. In previous animal studies, miR\u201020a\u20105p was found to be down\u2010expressed in low motile spermatozoa, implying its potential target of genes associated with cell apoptosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To investigate miR\u201020a\u20105p expression in blood plasma of patients suffering from non\u2010obstructive azoospermia (NOA), compared to normozoospermic controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Between January 2018 and December 2019, from 52 infertile couples eligible for the study, 24 couples were finally enrolled in this monocentric observational prospective pilot study. Patients were included into two groups: Group 1 comprised men with NOA (n\u00a0=\u00a014) and Group 2 fertile men partners of women with female tubal factor infertility (n\u00a0=\u00a010). All NOA patients underwent testicular sperm extraction. The expression of circulating miR\u201020a\u20105p in plasma samples was assessed by RT\u2010qPCR. A relative quantification strategy was adopted using the 2<jats:sup>\u2212\u0394Cq<\/jats:sup> method to calculate the target miR\u201020a\u20105p expression with respect to miR\u201016\u20105p as endogenous control.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Median blood plasma miR\u201020a\u20105p was significantly higher in patients affected by NOA (0.16 2<jats:sup>\u2212\u0394Ct<\/jats:sup>, range: 0.05\u20100.79 2<jats:sup>\u2212\u0394Ct<\/jats:sup>) than in fertile controls (0.06 2<jats:sup>\u2212\u0394Ct<\/jats:sup>, range: 0.04\u20100.10 2<jats:sup>\u2212\u0394Ct<\/jats:sup>), <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.001. MiR\u201020a\u20105p was positively correlated with follicle\u2010stimulating hormone (FSH) (<jats:italic>r<\/jats:italic><jats:sub>rho<\/jats:sub>\u00a0=\u00a0\u22120.490, <jats:italic>P<\/jats:italic>\u00a0=\u00a0.015) and luteinizing hormone (LH) (<jats:italic>r<\/jats:italic><jats:sub>rho<\/jats:sub>\u00a0=\u00a0\u22120.462, <jats:italic>P<\/jats:italic>\u00a0=\u00a0.023), and negatively correlated with serum total testosterone (TT) (<jats:italic>r<\/jats:italic><jats:sub>rho<\/jats:sub>\u00a0=\u00a0\u22120.534, <jats:italic>P<\/jats:italic>\u00a0=\u00a0.007) and right and left testicular size (<jats:italic>r<\/jats:italic><jats:sub>rho<\/jats:sub>\u00a0=\u00a0\u22120.473, <jats:italic>P<\/jats:italic>\u00a0=\u00a0.020 and <jats:italic>r<\/jats:italic><jats:sub>rho<\/jats:sub>\u00a0=\u00a0\u22120.471, <jats:italic>P<\/jats:italic>\u00a0=\u00a0.020, respectively). Successful sperm retrieval (SR) rate was 50.0%. Median value of miR\u201020a\u20105p did not differ significantly among patients with successful SR and those with negative SR. Testicular histological examination showed: hypospermatogenesis in 6\/14 (42.8%), maturation arrest in 4\/14 (28.6%), sertoli cell\u2010only syndrome in 4\/14 (28.6%). No significant differences in miR\u201020a\u20105p were found between histopathological patterns (<jats:italic>P<\/jats:italic>\u00a0&gt;\u00a0.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>MiR\u201020a\u20105p could represent a novel non\u2010invasive diagnostic biomarker of male infertility.<\/jats:p><\/jats:sec>","DOI":"10.1111\/andr.12816","type":"journal-article","created":{"date-parts":[[2020,5,14]],"date-time":"2020-05-14T08:53:00Z","timestamp":1589446380000},"page":"1256-1264","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Blood plasma miR\u201020a\u20105p expression as a potential non\u2010invasive diagnostic biomarker of male infertility: A pilot study"],"prefix":"10.1111","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7526-4025","authenticated-orcid":false,"given":"Gianmartin","family":"Cito","sequence":"first","affiliation":[{"name":"Department of 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