{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T11:06:45Z","timestamp":1788174405606,"version":"build-2803163510"},"reference-count":67,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2020,6,22]],"date-time":"2020-06-22T00:00:00Z","timestamp":1592784000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003093","name":"Ministry of Higher Education, Malaysia","doi-asserted-by":"publisher","award":["(Fundamental Research Grant Scheme: 203.PPSP.6171195)"],"award-info":[{"award-number":["(Fundamental Research Grant Scheme: 203.PPSP.6171195)"]}],"id":[{"id":"10.13039\/501100003093","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Andrology"],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Steroidogenesis decline is reported to be one of the mechanisms associated with obesity\u2010induced male factor subfertility\/infertility.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>We explored the possible preventive\/therapeutic effects of orlistat (a medication prescribed for weight loss) on obesity\u2010induced steroidogenesis and spermatogenesis decline.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>Twenty\u2010four adult male<jats:italic>Sprague Dawley<\/jats:italic>rats weighing 250\u2010300\u00a0g were randomized into four groups (n\u00a0=\u00a06\/group), namely; normal control, high\u2010fat diet, high\u2010fat diet plus orlistat preventive group and high\u2010fat diet plus orlistat treatment group. Orlistat (10\u00a0mg\/kg\/b.w.\/d suspended in distilled water) was either concurrently administered with high\u2010fat diet for 12\u00a0weeks (high\u2010fat diet plus orlistat preventive group) or administered from week 7\u201012 post\u2010 high\u2010fat diet feeding (high\u2010fat diet plus orlistat treatment group). Thereafter, serum, testes and epididymis were collected for analyses.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Obesity increased serum leptin and decreased adiponectin levels, decreased serum and intra\u2010testicular levels of follicle stimulating hormone, luteinising hormone and testosterone, sperm count, motility, viability, normal morphology and epididymal antioxidants, but increased epididymal malondialdehyde level and sperm nDNA fragmentation. Testicular mRNA transcript levels of androgen receptor, luteinizing hormone receptor, steroidogenic acute regulatory protein, cytochrome P450 enzyme (CYP11A1), 3\u03b2\u2010hydroxysteroid dehydrogenase and 17\u03b2\u2010hydroxysteroid dehydrogenase were significantly decreased in the testes of the high\u2010fat diet group. Further, the levels of steroidogenic acute regulatory protein protein and enzymatic activities of CYP11A1, 3\u03b2\u2010hydroxysteroid dehydrogenase and 17\u03b2\u2010hydroxysteroid dehydrogenase were also significantly decreased in the testes of the high\u2010fat diet group. Treatment with orlistat significantly decreased leptin and increased adiponectin levels, improved sperm parameters, decreased sperm DNA fragmentation, increased the levels of steroidogenic hormones, proteins and associated genes in high\u2010fat diet\u2010induced obese male rats, with the preventive group (high\u2010fat diet plus orlistat preventive group) having better results relative to the treatment group (high\u2010fat diet plus orlistat treatment group).<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion and Conclusion<\/jats:title><jats:p>Orlistat attenuated impaired spermatogenesis and steroidogenesis decline by up\u2010regulating steroidogenic genes. This may not be unconnected to its significant effect in lowering serum leptin levels, since the hormone is known to dampen fertility potential. Therefore, orlistat may improve fertility potential in overweight\/obese men.<\/jats:p><\/jats:sec>","DOI":"10.1111\/andr.12824","type":"journal-article","created":{"date-parts":[[2020,5,21]],"date-time":"2020-05-21T22:54:05Z","timestamp":1590101645000},"page":"1471-1485","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Orlistat attenuates obesity\u2010induced decline in steroidogenesis and spermatogenesis by up\u2010regulating steroidogenic genes"],"prefix":"10.1111","volume":"8","author":[{"given":"Joseph Bagi","family":"Suleiman","sequence":"first","affiliation":[{"name":"Department of Physiology School of Medical Sciences Universiti Sains Malaysia Kubang Kerian Kelantan Malaysia"},{"name":"Department of Physiology|Faculty of Basic Medical Sciences|College of Medical Sciences Akanu Ibiam Federal Polytechnic Unwana Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7616-9468","authenticated-orcid":false,"given":"Victor Udo","family":"Nna","sequence":"additional","affiliation":[{"name":"Department of Physiology Faculty of Basic Sciences College of Medical Sciences University of Calabar Calabar Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zaidatul Akmal","family":"Othman","sequence":"additional","affiliation":[{"name":"Department of Physiology School of Medical Sciences Universiti Sains Malaysia Kubang Kerian Kelantan Malaysia"},{"name":"Unit of Physiology Faculty of Medicine Universiti Sultan Zainal Abidin Kuala Terengganu Terengganu Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zaida","family":"Zakaria","sequence":"additional","affiliation":[{"name":"Department of Physiology School of Medical Sciences Universiti Sains Malaysia Kubang Kerian Kelantan Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ainul Bahiyah Abu","family":"Bakar","sequence":"additional","affiliation":[{"name":"Department of Physiology School of Medical Sciences Universiti Sains Malaysia Kubang Kerian Kelantan Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9333-1957","authenticated-orcid":false,"given":"Mahaneem","family":"Mohamed","sequence":"additional","affiliation":[{"name":"Department of Physiology School of Medical Sciences Universiti Sains Malaysia Kubang Kerian Kelantan Malaysia"},{"name":"Unit of Integrative Medicine School of Medical Sciences Universiti Sains Malaysia Kubang Kerian Kelantan Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,6,22]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2017.11.011"},{"key":"e_1_2_9_3_1","unstructured":"LevineAH MelansonDA MeadeJC.Inventors; GI Dynamics Inc assignee. Anti\u2010obesity devices. United States patent US 9 901 474. 2018 Feb 27."},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/and.13147"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/and.12888"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.5173\/ceju.2015.01.435"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.14143"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygcen.2017.07.013"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1530\/EJE-13-0802"},{"key":"e_1_2_9_10_1","first-page":"277","article-title":"The effect of quince leaf (Cydonia oblonga Miller) decoction on testes in hypercholesterolemic rabbits: a pilot study","volume":"10","author":"Ashrafi H","year":"2013","journal-title":"Afr J Tradit Complement Altern Med"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygcen.2011.04.015"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.2164\/jandrol.05124"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.4161\/oxim.2.2.8177"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1387\/ijdb.130146ja"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diabres.2004.05.012"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.ijo.0802281"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/oby.2011.143"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2017.06.035"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.14310\/horm.2002.11190"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bjbas.2014.05.002"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.2017.11"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11418-011-0597-8"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.2002.282.1.R46"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.4103\/0253-7613.108288"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/andr.12567"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0064751"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/608512"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbi.2014.04.005"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2008.11.006"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)42083-8"},{"key":"e_1_2_9_31_1","doi-asserted-by":"crossref","first-page":"2747","DOI":"10.1681\/ASN.V12122747","article-title":"Oxidative stress and endothelial function in chronic renal failure","volume":"12","author":"Annuk M","year":"2001","journal-title":"J Am Soc Nephrol"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.2000.00212.x"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.54.5.356"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms12095508"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1159\/000178170"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2004-0339"},{"key":"e_1_2_9_38_1","first-page":"615","article-title":"Lifestyle factors in deteriorating male reproductive health","volume":"47","author":"Kumar S","year":"2009","journal-title":"Indian J Exp Biol"},{"key":"e_1_2_9_39_1","first-page":"551","article-title":"Exercise protects against obesity induced semen abnormalities via downregulating stem cell factor, upregulating Ghrelin and normalizing oxidative stress","volume":"13","author":"Alhashem F","year":"2014","journal-title":"EXCLI J"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1186\/1758-5996-3-17"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2605.2010.01092.x"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1186\/1477-7827-9-32"},{"key":"e_1_2_9_43_1","first-page":"205","article-title":"Safety and mechanism of action of orlistat (tetrahydrolipstatin) as the first local antiobesity drug","volume":"2","author":"Al\u2010Suwailem A","year":"2006","journal-title":"J Appl Sci Res"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(07)60033-6"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2017.00807"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/ijo.2017.280"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1089\/jmf.2010.1152"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1097\/MED.0b013e3282f1cfdc"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1043-2760(00)00352-0"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.84.10.3673"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00033.2011"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1002\/jsfa.6094"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1038\/oby.2006.319"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1530\/eje.0.1430293"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0303-7207(02)00008-4"},{"key":"e_1_2_9_56_1","first-page":"e13433","article-title":"Leptin and reproductive dysfunction in obese men","volume":"52","author":"Almabhouh FA","year":"2019","journal-title":"Andrologia"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12958-018-0368-4"},{"key":"e_1_2_9_58_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2014\/190945","article-title":"Leptin level and oxidative stress contribute to obesity\u2010induced low testosterone in murine testicular tissue","volume":"2014","author":"Zhao J","year":"2014","journal-title":"Oxid Med Cell Longev"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1111\/and.12325"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2009.07.995"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12020-013-0102-0"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2007-1582"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2007.07.1292"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.acthis.2009.10.004"},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10815-015-0506-2"},{"key":"e_1_2_9_66_1","doi-asserted-by":"publisher","DOI":"10.1097\/XCE.0000000000000057"},{"key":"e_1_2_9_67_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.26046"},{"key":"e_1_2_9_68_1","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1515\/hmbci-2015-0046","article-title":"Roles of leptin, adiponectin and resistin in the transcriptional regulation of steroidogenic genes contributing to decreased Leydig cells function in obesity","volume":"24","author":"Roumaud P","year":"2015","journal-title":"Horm Mol Biol Clin Investig"}],"container-title":["Andrology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fandr.12824","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/andr.12824","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/andr.12824","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/andr.12824","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,6]],"date-time":"2024-08-06T05:32:38Z","timestamp":1722922358000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/andr.12824"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,6,22]]},"references-count":67,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2020,9]]}},"alternative-id":["10.1111\/andr.12824"],"URL":"https:\/\/doi.org\/10.1111\/andr.12824","archive":["Portico"],"relation":{},"ISSN":["2047-2919","2047-2927"],"issn-type":[{"value":"2047-2919","type":"print"},{"value":"2047-2927","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,6,22]]},"assertion":[{"value":"2020-01-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-05-17","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-06-22","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}