{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T11:10:38Z","timestamp":1784632238611,"version":"3.55.0"},"reference-count":34,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2020,7,12]],"date-time":"2020-07-12T00:00:00Z","timestamp":1594512000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Research Foundation of the Department of Human Resources and Social Security of Sichuan Province of Returned Scholars","award":["2019-76"],"award-info":[{"award-number":["2019-76"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Andrology"],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The number of erectile dysfunction (ED) patients is increasing annually. How to improve the effectiveness of ED treatment is an important issue for the field of andrology.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To investigate whether low androgen status impairs the erectile function of rats by regulated endothelial nitric oxide synthase (eNOS) uncoupling.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>Thirty\u2010six 8\u2010week\u2010old male Sprague Dawley (SD) rats were randomly divided into six groups as follows: 4\u2010week sham\u2010operated group (4w\u2010sham), 4\u2010week castration group (4w\u2010cast), 4\u2010week castration\u00a0+\u00a0testosterone (T) group (4w\u2010cast\u00a0+\u00a0T), 8\u2010week sham\u2010operated group (8w\u2010sham), 8\u2010week castration group (8w\u2010cast), and 8\u2010week castration\u00a0+\u00a0T group (8w\u2010cast\u00a0+\u00a0T). Three mg\/kg of T was subcutaneously injected every other day in castration\u00a0+\u00a0T groups. The ratio of the maximum intracavernous pressure\/the mean arterial pressure (ICPmax\/MAP), the level of serum T, dihydrobiopterin(BH<jats:sub>2<\/jats:sub>), tetrahydrobiopterin (BH<jats:sub>4<\/jats:sub>), nitric oxygen(NO), 3\u2010nitrotyrosine(3NT), dihydrofolate reductase (DHFR), guanosine triphosphate cyclohydrolase 1 (GTPCH1), nicotinamide adenine dinucleotide phosphate oxidase 2 (NOX2), and eNOS monomers\/dimers in the corpus cavernosum were detected.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The ratio of ICPmax\/MAP and BH<jats:sub>4<\/jats:sub>\/BH<jats:sub>2<\/jats:sub>, the level of serum T, NO, and GTPCH1 decreased significantly in castration groups compared with sham\u2010operated groups and castration\u00a0+\u00a0T groups (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.05) and decreased significantly in 8w\u2010cast group compared with 4w\u2010cast group (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.05). The expression of 3NT and NOX2 and the ratio of eNOS monomers\/dimers increased significantly in castration groups compared with sham\u2010operated groups and castration\u00a0+\u00a0T groups (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.01) and increased significantly in 8w\u2010cast group compared with 4w\u2010cast group (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.01). The expression of DHFR in 4w\u2010cast group was significantly higher than that in 4w\u2010sham group and 4w\u2010cast\u00a0+\u00a0T group (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.01) and in 8w\u2010cast group was significantly lower than that in 8w\u2010sham group and 8w\u2010cast\u00a0+\u00a0T group (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.01).<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion and Conclusion<\/jats:title><jats:p>Low androgen status induces eNOS uncoupling by reducing BH<jats:sub>4<\/jats:sub>\/BH<jats:sub>2<\/jats:sub> and increasing 3NT. Due to the decreased NO production, the erectile function of the rats was impaired.<\/jats:p><\/jats:sec>","DOI":"10.1111\/andr.12844","type":"journal-article","created":{"date-parts":[[2020,6,28]],"date-time":"2020-06-28T10:59:43Z","timestamp":1593341983000},"page":"1875-1883","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Low androgen status inhibits erectile function by inducing eNOS uncoupling in rat corpus cavernosum"],"prefix":"10.1111","volume":"8","author":[{"given":"Wenju","family":"Xiong","sequence":"first","affiliation":[{"name":"Department of Urology the Affiliated Hospital of Southwest Medical University Luzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangjun","family":"Kong","sequence":"additional","affiliation":[{"name":"Department of Urology the Affiliated Hospital of Southwest Medical University Luzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Thyroid Surgery the Affiliated Hospital of Southwest Medical University Luzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhihui","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Pathology the Affiliated Hospital of Southwest Medical University Luzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1849-3643","authenticated-orcid":false,"given":"Rui","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Urology the Affiliated Hospital of Southwest Medical University Luzhou China"},{"name":"Nephropathy Clinical Medical Research Center of Sichuan Province Luzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,7,12]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.urology.2013.03.034"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.25889"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40266-018-0528-4"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.11-181057"},{"issue":"10","key":"e_1_2_7_6_1","doi-asserted-by":"crossref","first-page":"3377","DOI":"10.1534\/g3.118.200509","article-title":"Deletion of GIT1 impacts eNOS activity to aggravate sFlt\u20101\u2013induced preeclampsia phenotype in mice","volume":"8","author":"Zhang S","year":"2018","journal-title":"G3: Genes \u2010 Genomes \u2010 Genetics"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/and.13167"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.healun.2019.03.004"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/s10020-018-0010-0"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.041483"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbi.2019.03.010"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2018.03.011"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1203\/PDR.0b013e318204dcb8"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/and.13084"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/andr.12313"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1743-6109.2010.01880.x"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/ijir.2011.2"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.urology.2018.03.018"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/and.13075"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.urology.2019.08.025"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1995.tb00038.x"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.14130"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.maturitas.2018.04.004"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.136.4.7534702"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod61.4.1012"},{"issue":"2","key":"e_1_2_7_26_1","first-page":"161","article-title":"Uncoupling of endothelial NO synthase in atherosclerosis and vascular disease","volume":"13","author":"Li H","year":"2013","journal-title":"CurrOpin Pharmacol"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.7192"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsxm.2017.11.001"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.2000"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/and.13173"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1089\/neu.2015.4340"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0105479"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.2174\/13816128113196660745"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/2354870"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.14715\/cmb\/2016.62.13.10"}],"container-title":["Andrology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fandr.12844","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/andr.12844","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/andr.12844","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/andr.12844","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,1]],"date-time":"2023-09-01T20:55:09Z","timestamp":1693601709000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/andr.12844"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,7,12]]},"references-count":34,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2020,11]]}},"alternative-id":["10.1111\/andr.12844"],"URL":"https:\/\/doi.org\/10.1111\/andr.12844","archive":["Portico"],"relation":{},"ISSN":["2047-2919","2047-2927"],"issn-type":[{"value":"2047-2919","type":"print"},{"value":"2047-2927","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,7,12]]},"assertion":[{"value":"2020-02-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-06-17","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-07-12","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}