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However, this temperature is challenging for some ejaculates or boars.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>The present study aimed to identify putative biomarkers for semen resistance to hypothermic storage at 5\u00b0C by comparing the seminal plasma proteomes of boars with high and low seminal resistance to preservation at 5\u00b0C.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>From an initial group of 34 boars, 15 were selected based on the following criteria: ejaculate with \u226420% abnormal spermatozoa and at least 70% progressive motility at 120\u00a0hours of storage at 17\u00b0C. Then, based on the response to semen hypothermic storage at 5\u00b0C, boars were classified into two categories: high resistance\u2014progressive motility of &gt;75% in the three collections (n\u00a0=\u00a03); and low resistance\u2014progressive motility of &lt;75% in the three collections (n\u00a0=\u00a03). Seminal plasma proteins were analyzed in pools, and differential proteomics was performed using Multidimensional Protein Identification Technology.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Progressive motility was lower at 120\u00a0hours of storage in low resistance, compared to high resistance boars (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.05). Acrosome and plasma membrane integrity were not affected by the boar category, storage time, or their interaction (<jats:italic>P<\/jats:italic>\u00a0\u2265\u00a0.104). Sixty\u2010five proteins were considered for differential proteomics. Among the differentially expressed and exclusive proteins, the identification of proteins such cathepsin B, legumain, and cystatin B suggests significant changes in key enzymes (eg, metalloproteinases) involved in spermatogenesis, sperm integrity, and fertilizing potential.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion and Conclusion<\/jats:title><jats:p>Differences in the seminal plasma suggest that proteins involved in the proteolytic activation of metalloproteinases and proteins related to immune response modulation could disrupt key cellular pathways during spermatogenesis and epididymal maturation, resulting in altered resistance to chilling injury. Further in vivo studies focusing on the immunological crosstalk between epithelial cells and gametes might explain how the immune regulators influence sperm resistance to hipothermic storage.<\/jats:p><\/jats:sec>","DOI":"10.1111\/andr.12869","type":"journal-article","created":{"date-parts":[[2020,7,20]],"date-time":"2020-07-20T03:20:45Z","timestamp":1595215245000},"page":"1907-1922","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Differential seminal plasma proteome signatures of boars with high and low resistance to hypothermic semen preservation at 5\u00b0C"],"prefix":"10.1111","volume":"8","author":[{"given":"Tila de Alcantara","family":"Menezes","sequence":"first","affiliation":[{"name":"Setor de Su\u00ednos Faculdade de Veterin\u00e1ria Universidade Federal do Rio Grande do Sul Porto Alegre 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