{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T05:35:10Z","timestamp":1791956110210,"version":"4.3.4"},"reference-count":38,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,9,10]],"date-time":"2020-09-10T00:00:00Z","timestamp":1599696000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2019R1A2C2002414"],"award-info":[{"award-number":["2019R1A2C2002414"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Andrology"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Radical prostatectomy induces some degree of cavernous nerve injury (CNI) and causes denervation\u2010induced pathologic changes in cavernous vasculature, regardless of the advances in surgical techniques and robotic procedures. The precursor for nerve growth factor (proNGF) is known to be involved in neuronal cell apoptosis and microvascular dysfunction through its receptor p75<jats:sup>NTR<\/jats:sup>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To determine the expression of proNGF\/p75<jats:sup>NTR<\/jats:sup> and the efficacy of proNGF neutralizing antibody (anti\u2010proNGF\u2010Ab) in a mouse model of ED induced by CNI.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Age\u2010matched 12\u2010week\u2010old C57BL\/6 mice were distributed into three groups: sham group and bilateral CNI group treated with intracavernous injections of PBS (20\u00a0\u03bcL) or of anti\u2010proNGF\u2010Ab (20\u00a0\u00b5g in 20\u00a0\u03bcL of PBS) on days \u22123 and 0. Two weeks after treatment, erectile function was measured by electrical stimulation of cavernous nerve. Penis tissues from a separate group of animals were harvested for further analysis. We also determined the efficacy of anti\u2010proNGF\u2010Ab on neural preservation in major pelvic ganglion (MPG) ex vivo.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We observed increased penile expression of proNGF and p75<jats:sup>NTR<\/jats:sup> after CNI. Intracavernous administration of anti\u2010proNGF\u2010Ab increased nNOS and neurofilament expression probably by enhancing the production of neurotrophic factors, such as neurotrophin\u20103, NGF, and brain\u2010derived neurotrophic factor. Anti\u2010proNGF\u2010Ab preserved the integrity of cavernous sinusoids, such as pericytes, endothelial cells, and endothelial cell\u2010to\u2010cell junctions, possibly by controlling angiogenic factors (angiopoietin\u20101, angiopoietin\u20102, and vascular endothelial growth factor) and induced endogenous eNOS phosphorylation in CNI mice. And finally, treatment with anti\u2010proNGF\u2010Ab rescued erectile function in CNI mice. Anti\u2010proNGF\u2010Ab also enhanced neurite sprouting from MPG exposed to lipopolysaccharide.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion and Conclusion<\/jats:title><jats:p>The preservation of damaged cavernous neurovasculature through inhibition of the proNGF\/p75<jats:sup>NTR<\/jats:sup> pathway may be a novel strategy to treat radical prostatectomy\u2010induced erectile dysfunction.<\/jats:p><\/jats:sec>","DOI":"10.1111\/andr.12873","type":"journal-article","created":{"date-parts":[[2020,7,22]],"date-time":"2020-07-22T06:54:29Z","timestamp":1595400869000},"page":"329-341","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Neutralizing antibody to proNGF rescues erectile function by regulating the expression of neurotrophic and angiogenic factors in a mouse model of cavernous nerve 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