{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T17:29:19Z","timestamp":1790616559080,"version":"4.1.0"},"reference-count":31,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,9,9]],"date-time":"2020-09-09T00:00:00Z","timestamp":1599609600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81000079"],"award-info":[{"award-number":["81000079"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81170165"],"award-info":[{"award-number":["81170165"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81870959"],"award-info":[{"award-number":["81870959"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Andrology"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Male infertility is a major issue in human reproduction health, yet known genetic factors are only responsible for a small fraction of cases. TSGA10 is a testis\u2010specific protein that is highly conserved among different species. A previous study has reported a homozygous mutation in <jats:italic>TSGA10<\/jats:italic> in a male infertile patient; however, function analysis of <jats:italic>Tsga10<\/jats:italic> genes in knockout mice has not yet been undertaken.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>The aim of the present work was to analyse the function of TSGA10 protein in the spermatogenesis of <jats:italic>Tsga10<jats:sup>+\/\u2212<\/jats:sup><\/jats:italic> mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p><jats:italic>Tsga10<jats:sup>+\/\u2212<\/jats:sup><\/jats:italic> mice were generated by CRISPR\/Cas9 technology, in vitro fertilization (IVF), western blot, co\u2010immunoprecipitation and other methods were used to the function analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Heterozygous <jats:italic>Tsga10<\/jats:italic> male mice created by CRISPR\/Cas9 were infertile and presented significantly reduced sperm motility because of disordered mitochondrial sheath formation. Furthermore, TSGA10 can interact with GRP78 and NSUN2, which are associated with peri\u2010implantation lethality and the gonadotropin\u2010releasing hormone (GnRH) network.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion and conclusion<\/jats:title><jats:p>We demonstrate that deficiency of <jats:italic>Tsga10<\/jats:italic> gene can lead to male infertility in mice. TSGA10 is involved in the correct arrangement of mitochondrial sheath in spermatozoa. Future studies on TSGA10 include an in\u2010depth exploration of the underlying mechanisms of TSGA10 in spermatogenesis, early embryonic development and GnRH network.<\/jats:p><\/jats:sec>","DOI":"10.1111\/andr.12889","type":"journal-article","created":{"date-parts":[[2020,8,13]],"date-time":"2020-08-13T14:00:21Z","timestamp":1597327221000},"page":"368-375","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["<i>Tsga10<\/i> is essential for arrangement of mitochondrial sheath and male fertility in mice"],"prefix":"10.1111","volume":"9","author":[{"given":"Geng","family":"Luo","sequence":"first","affiliation":[{"name":"Key Laboratory of Molecular Biophysics of the Ministry of Education College of Life Science and Technology Center for Human Genome Research Huazhong University of Science and Technology Wuhan 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