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Primary rat Sertoli cells were isolated with enzymatic hydrolysis. Exosomes were isolated from primary rat Sertoli cells, rat epididymis tissue, and human seminal plasma with polymer\u2010based precipitation method. Exosomal microRNAs were quantified with qPCR. Inhibin\u2010B was detected with enzyme immunoassay. The correlation analysis between microRNA and inhibin\u2010B was evaluated with Spearman's correlation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We screened 12 previously reported hypoxia\u2010responsive microRNAs in the primary rat Sertoli cells and found that 4 exosomal microRNAs increased significantly in response to in vitro hypoxia treatment (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.05). Of the 4 microRNAs, only miR\u2010210\u20103p was upregulated in the rats with experimental varicocoele (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.01). In the patients with varicocoele, we found that seminal exosomal miR\u2010210\u20103p significantly increased in patients with grade II and III varicocoele (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.01), and miR\u2010210\u20103p negatively correlated with sperm count (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.01) and seminal inhibin\u2010B expression (<jats:italic>r<\/jats:italic>\u00a0=\u00a0\u22120.39, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.01). For the 30 patients with microsurgical varicocelectomy, the operation notably decreased miR\u2010210\u20103p (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.01).<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion and Conclusion<\/jats:title><jats:p>Seminal exosomal miR\u2010210\u20103p may be a novel, sensitive, and non\u2010invasive biomarker of Sertoli cell damage in varicocoele.<\/jats:p><\/jats:sec>","DOI":"10.1111\/andr.12913","type":"journal-article","created":{"date-parts":[[2020,10,1]],"date-time":"2020-10-01T02:03:40Z","timestamp":1601517820000},"page":"451-459","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":39,"title":["Seminal exosomal miR\u2010210\u20103p as a potential marker of Sertoli cell damage in Varicocele"],"prefix":"10.1111","volume":"9","author":[{"given":"Yi","family":"Ma","sequence":"first","affiliation":[{"name":"Center for Reproductive Medicine Ren Ji Hospital School of Medicine Shanghai Jiao Tong 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