{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T22:19:49Z","timestamp":1782339589308,"version":"3.54.5"},"reference-count":14,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2021,2,17]],"date-time":"2021-02-17T00:00:00Z","timestamp":1613520000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Andrology"],"published-print":{"date-parts":[[2021,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Physiological selection of spermatozoa for ICSI (PICSI) is a sperm selection method based on sperm binding to hyaluronic acid. Previous studies on the effect of hyaluronic acid binding assays on fertilization and embryo quality have shown inconsistent results. Previous sibling oocyte studies have not found a significant improvement in fertilization or embryo development with hyaluronic acid binding assays.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To compare fertilization and embryo development between standard intracytoplasmic sperm injection (ICSI) and PICSI in sibling oocytes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>This is a retrospective analysis of all in vitro fertilization (IVF) cycles between January 2017 and April 2020 in which sibling oocytes were randomly fertilized by both ICSI and PICSI. Fertilization rate and the rate of embryos eligible for transfer were compared.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Forty\u2010five IVF cycles, in which 257 oocytes were fertilized with PICSI and 294 with standard ICSI, were compared. Most of the patients included in the study had previous failures of fertilization, poor embryonic development, implantation failure, or miscarriage. All but two of the patients had at least one previous unsuccessful IVF cycle. Both fertilization rates (71% vs. 83%) and transfer eligible embryo rates (38% vs. 51%) were significantly higher in PICSI fertilized oocytes (<jats:italic>p<\/jats:italic>\u00a0=\u00a00.008 and <jats:italic>p<\/jats:italic>\u00a0=\u00a00.01 respectively).<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p>Our study is the largest sibling oocyte study comparing ICSI and PICSI, and the first to find a significant improvement in fertilization and embryo quality with PICSI using sibling oocytes. The fact our cohort included almost exclusively couples with previous unsuccessful IVF cycles might suggest that PICSI should be used in selected cases.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>PICSI improves fertilization rates and transfer eligible embryo rates in sibling oocytes in a selected study group.<\/jats:p><\/jats:sec>","DOI":"10.1111\/andr.12982","type":"journal-article","created":{"date-parts":[[2021,2,2]],"date-time":"2021-02-02T23:00:46Z","timestamp":1612306846000},"page":"873-877","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Conventional ICSI vs. physiological selection of spermatozoa for ICSI (picsi) in sibling oocytes"],"prefix":"10.1111","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9346-8814","authenticated-orcid":false,"given":"Michal","family":"Novoselsky Persky","sequence":"first","affiliation":[{"name":"IVF Unit Department of Obstetrics and Gynecology Hadassah Mt. Scopus Hebrew University Medical Center Jerusalem Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anat","family":"Hershko\u2010Klement","sequence":"additional","affiliation":[{"name":"IVF Unit Department of Obstetrics and Gynecology Hadassah Mt. Scopus Hebrew University Medical Center Jerusalem Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amy","family":"Solnica","sequence":"additional","affiliation":[{"name":"IVF Unit Department of Obstetrics and Gynecology Hadassah Mt. Scopus Hebrew University Medical Center Jerusalem Israel"},{"name":"Henrietta Szold School of Nursing Faculty of Medicine Hebrew University Jerusalem Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuval","family":"Bdolah","sequence":"additional","affiliation":[{"name":"IVF Unit Department of Obstetrics and Gynecology Shaare Zedek Medical center Jerusalem Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arye","family":"Hurwitz","sequence":"additional","affiliation":[{"name":"IVF Unit Department of Obstetrics and Gynecology Shaare Zedek Medical center Jerusalem Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mali","family":"Ketzin El Gilad","sequence":"additional","affiliation":[{"name":"IVF Unit Department of Obstetrics and Gynecology Hadassah Mt. Scopus Hebrew University Medical Center Jerusalem Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iris","family":"Nefesh","sequence":"additional","affiliation":[{"name":"IVF Unit Department of Obstetrics and Gynecology Hadassah Mt. Scopus Hebrew University Medical Center Jerusalem Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Efrat","family":"Esh\u2010Broder","sequence":"additional","affiliation":[{"name":"IVF Unit Department of Obstetrics and Gynecology Hadassah Mt. Scopus Hebrew University Medical Center Jerusalem Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,2,17]]},"reference":[{"issue":"2","key":"e_1_2_8_2_1","first-page":"194","article-title":"The ICSI procedure from past to future: a systematic review of the more controversial aspects","volume":"22","author":"Rubino P","year":"2016","journal-title":"Human Reprod"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2019.03.024"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/des417"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.5935\/1518-0557.20170002"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.3109\/19396368.2014.948102"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rbmo.2015.12.001"},{"key":"e_1_2_8_8_1","first-page":"CD010461","article-title":"Advanced sperm selection techniques for assisted reproduction","volume":"7","author":"Lepine S","year":"2019","journal-title":"Cochrane Database Syst Rev"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(18)32989-1"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.3346\/jkms.2012.27.12.1569"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1080\/14647273.2017.1366077"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rbmo.2009.09.022"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1472-6483(10)60155-1"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/der037"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(00)00518-5"}],"container-title":["Andrology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/andr.12982","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/andr.12982","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/andr.12982","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,1]],"date-time":"2023-09-01T05:19:35Z","timestamp":1693545575000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/andr.12982"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,2,17]]},"references-count":14,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2021,5]]}},"alternative-id":["10.1111\/andr.12982"],"URL":"https:\/\/doi.org\/10.1111\/andr.12982","archive":["Portico"],"relation":{},"ISSN":["2047-2919","2047-2927"],"issn-type":[{"value":"2047-2919","type":"print"},{"value":"2047-2927","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,2,17]]},"assertion":[{"value":"2020-10-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-01-26","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-02-17","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}