{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T00:39:06Z","timestamp":1747183146836,"version":"3.40.5"},"reference-count":29,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,8,13]],"date-time":"2021-08-13T00:00:00Z","timestamp":1628812800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81871147","82071639"],"award-info":[{"award-number":["81871147","82071639"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Andrology"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Intracavernous pressure measurement following cavernous nerve electrostimulation has been extensively adopted for the evaluation of erectile function in animals. However, the effect of measurement time and acidosis during anesthesia is still lacking.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To explore the effect of measurement time and acidosis during anesthesia.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>Fifty\u2010six male Sprague\u2010Dawley rats were used and anesthetized by a spontaneous inhalation of isoflurane. In the first step, rats were randomly divided into four groups: a control group and three time\u2010delayed measurement groups (intracavernous pressure measurement beginning at 15, 30, and 45\u00a0min after cavernous nerve exposure). In the second step, rats were randomly divided into three groups: a control group and two time\u2010delayed measurement groups. Two intravenous fluid support strategies were used in time\u2010delayed measurement groups: a normal saline solution and an isotonic Na<jats:sub>2<\/jats:sub>CO<jats:sub>3<\/jats:sub> solution.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Isoflurane\u2010anesthetized rats developed systemic acidosis that worsens with time during intracavernous pressure measurement, which results in a significant decrease in the maximum intracavernous pressure value, intracavernous pressure\/mean arterial pressure ratio, and total intracavernous pressure measured. The Na<jats:sub>2<\/jats:sub>CO<jats:sub>3<\/jats:sub> infusion could effectively correct acidosis. The decrease in intracavernous pressure was related to the reduced nitric oxide synthase activity, decreased cyclic guanosine monophosphate concentration, and reactive oxygen species activation in rat penis under acidosis conditions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion and conclusion<\/jats:title><jats:p>Prolonged isoflurane anesthesia\u2010induced acidosis markedly depresses the erectile response to cavernous nerve electrostimulation in rats. In this situation, it is recommended to supplement with a Na<jats:sub>2<\/jats:sub>CO<jats:sub>3<\/jats:sub> infusion to maintain a normal acid\u2010base balance.<\/jats:p><\/jats:sec>","DOI":"10.1111\/andr.13085","type":"journal-article","created":{"date-parts":[[2021,8,1]],"date-time":"2021-08-01T09:49:46Z","timestamp":1627811386000},"page":"143-153","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Prolonged isoflurane anesthesia\u2010induced acidosis decreases penile intracavernous pressure in rats"],"prefix":"10.1111","volume":"10","author":[{"given":"Botao","family":"Yu","sequence":"first","affiliation":[{"name":"Andrology Laboratory, West China Hospital Sichuan University  Chengdu China"},{"name":"Department of Urology, West China Hospital Sichuan University  Chengdu 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